Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Ch,
Gp,
Ha,
Pm,
Rb,
ShSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Gp, Ha, Pm, Rb, Sh
WB, Simple Western, Flow, ICC/IF, IHC, IP, KD
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IP, KD
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - analysis of Hsp47 in NIH/3T3 cell lysate (20ug/lane) using anti-Hsp47 antibody. 5x10^4 NIH/3T3 cells were seeded in 6-well plate and transfected with 50nM of HSP47 siRNA the following day. Total protein was harvested 72h after transfection. Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Western-Blot-NBP1-97491-img0006.jpg "Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - analysis of Hsp47 in NIH/3T3 cell lysate (20ug/lane) using anti-Hsp47 antibody. 5x10^4 NIH/3T3 cells were seeded in 6-well plate and transfected with 50nM of HSP47 siRNA the following day. Total protein was harvested 72h after transfection. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] - WNT and BMP signaling pathways play crucial roles in regeneration. a-d Real-time PCR for WNT and BMP signals, including Wnt7b Dual immunostaining for K15 and phospho-Smad1/5 (pSmad1/5) revealed the nuclear staining of pSmad1/5 in hair stem cells at day 7 when skin was stretched (arrow). Scale bar?=?50?um. h, i Immunostaining and confocal microscope. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-09402-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunocytochemistry-Immunofluorescence-NBP1-97491-img0007.jpg)
![Immunohistochemistry-Frozen: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Hsp47 antibody in guinea pig skin (subdermis). Image from verified customer review.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunohistochemistry-Frozen-NBP1-97491-img0004.jpg)
![Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of HSP47, mAb (M16.10A1): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO (heat shocked); Lane 6: HeLa (heat shocked).](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Western-Blot-NBP1-97491-img0003.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of paraffin-embedded tissue section of rat carotid artery 14 days after balloon-injury to the artery, stained using Hsp47 (Colligin) mAb (M16.10A1).](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunohistochemistry-Paraffin-NBP1-97491-img0001.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of human breast cancer tissue immunohistochemically stained using Hsp47 mAb (M16.10A1).](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Immunohistochemistry-Paraffin-NBP1-97491-img0002.jpg)
![Simple Western: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Simple Western lane view shows a specific band for Hsp47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Hsp47-Antibody-M16-10A1-Simple-Western-NBP1-97491-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] - WNT & BMP signaling pathways play crucial roles in regeneration. a–d Real-time PCR for WNT & BMP signals, including Wnt7b (a), Wnt10a (b), Lef1 (c), & Bmp2 (d) in response to stretch (day 1 & day 7) & release of stretch (day 9); n = 6 for each group. e Schematic of hair cycle activator & inhibitor in response to strain alteration. f Dual immunostaining for K15 & beta -catenin revealed nuclear staining of beta -catenin in hair follicles at day 9 & day 14 when stretch was released. Scale bar = 50 μm. g Dual immunostaining for K15 & phospho-Smad1/5 (pSmad1/5) revealed the nuclear staining of pSmad1/5 in hair stem cells at day 7 when skin was stretched (arrow). Scale bar = 50 μm. h, i Immunostaining (h) & confocal microscope (i) revealed co-localization of HSP47 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (h) or 10 μm (i). j, k Immunostaining (j) & confocal microscope (k) revealed co-localization of Perilipin-1 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (j) or 10 μm (k). Statistical significance was determined using ANOVA followed by a Bonferroni post hoc test. Data are presented as means ± SEM. *p < 0.05. **p < 0.01. ***p < 0.001. Source data are provided as a Source Data file. *Autofluorescence of hair shafts in g, h Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30944305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97491_mouse-monoclonal-hsp47-antibody-m16-10a1-310202416121964.jpg)
![Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] - WNT & BMP signaling pathways play crucial roles in regeneration. a–d Real-time PCR for WNT & BMP signals, including Wnt7b (a), Wnt10a (b), Lef1 (c), & Bmp2 (d) in response to stretch (day 1 & day 7) & release of stretch (day 9); n = 6 for each group. e Schematic of hair cycle activator & inhibitor in response to strain alteration. f Dual immunostaining for K15 & beta -catenin revealed nuclear staining of beta -catenin in hair follicles at day 9 & day 14 when stretch was released. Scale bar = 50 μm. g Dual immunostaining for K15 & phospho-Smad1/5 (pSmad1/5) revealed the nuclear staining of pSmad1/5 in hair stem cells at day 7 when skin was stretched (arrow). Scale bar = 50 μm. h, i Immunostaining (h) & confocal microscope (i) revealed co-localization of HSP47 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (h) or 10 μm (i). j, k Immunostaining (j) & confocal microscope (k) revealed co-localization of Perilipin-1 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (j) or 10 μm (k). Statistical significance was determined using ANOVA followed by a Bonferroni post hoc test. Data are presented as means ± SEM. *p < 0.05. **p < 0.01. ***p < 0.001. Source data are provided as a Source Data file. *Autofluorescence of hair shafts in g, h Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30944305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97491_mouse-monoclonal-hsp47-antibody-m16-10a1-31020241613615.jpg)
![Western Blot: Hsp47 Antibody [NBP1-88172] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SERPINH1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Western-Blot-NBP1-88172-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Hsp47 Antibody [NBP1-88172] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-SERPINH1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Hsp47 Antibody [NBP1-88172] - Staining of human cell line U-2 OS shows positivity in endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88172-img0003.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP1-88172] - Staining of human vulva/anal skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Immunohistochemistry-Paraffin-NBP1-88172-img0009.jpg)
![Western Blot: Hsp47 Antibody [NBP1-88172] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Western-Blot-NBP1-88172-img0008.jpg)
![Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in rat brain.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Western-Blot-H00000871-M01-img0008.jpg "Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in rat brain.")
![Immunohistochemistry-Paraffin: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - Immunoperoxidase of monoclonal antibody to SERPINH1 on formalin-fixed paraffin-embedded human ovary cancer. [antibody concentration 5 ug/ml]](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Immunohistochemistry-Paraffin-H00000871-M01-img0002.jpg)
![Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2 Analysis of SERPINH1 expression in HeLa.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Western-Blot-H00000871-M01-img0005.jpg)
![Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in MCF-7.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Western-Blot-H00000871-M01-img0006.jpg)
![Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in human pancreas.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Western-Blot-H00000871-M01-img0007.jpg)
![Sandwich ELISA: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - Detection limit for recombinant GST tagged SERPINH1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1D2-1A6-Sandwich-ELISA-H00000871-M01-img0004.jpg)
![Western Blot: Hsp47 Antibody [NBP2-16893] - A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 10% SDS-PAGE Hsp47 antibody Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Western-Blot-NBP2-16893-img0007.jpg "Western Blot: Hsp47 Antibody [NBP2-16893] - A. 30 ug U87-MG whole cell lysate/extract B. 30 ug SK-N-SH whole cell lysate/extract C. 30 ug IMR32 whole cell lysate/extract D. 30 ug SK-N-AS whole cell lysate/extract 10% SDS-PAGE Hsp47 antibody Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893] - Sample: Paraffin-embedded mouse kidney. Hsp47 antibody Internal dilution: 1:500.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Immunohistochemistry-Paraffin-NBP2-16893-img0003.jpg)
![Western Blot: Hsp47 Antibody [NBP2-16893] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:500.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Western-Blot-NBP2-16893-img0001.jpg)
![Western Blot: Hsp47 Antibody [NBP2-16893] - A. 30 ug A431 whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10% SDS-PAGE Hsp47 antibody Internal dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Western-Blot-NBP2-16893-img0006.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody [NBP2-16893] - Sample: Paraffin-embedded human breast carcinoma. Hsp47 antibody Internal dilution: 1:500.](http://images.novusbio.com/fullsize/Hsp47-Antibody-Immunohistochemistry-Paraffin-NBP2-16893-img0004.jpg)
![Western Blot: Hsp47 Antibody (3K3H1) [NBP3-15430] - Western blot analysis of extracts of various cell lines, using Hsp47 Rabbit mAb (NBP3-15430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Hsp47-Antibody-3K3H1-Western-Blot-NBP3-15430-img0003.jpg "Western Blot: Hsp47 Antibody (3K3H1) [NBP3-15430] - Western blot analysis of extracts of various cell lines, using Hsp47 Rabbit mAb (NBP3-15430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (3K3H1) [NBP3-15430] - Immunofluorescence analysis of NIH-3T3 cells using Hsp47 Rabbit mAb (NBP3-15430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Hsp47-Antibody-3K3H1-Immunocytochemistry-Immunofluorescence-NBP3-15430-img0002.jpg)
![Immunohistochemistry-Paraffin: Hsp47 Antibody (3K3H1) [NBP3-15430] - Immunohistochemistry of paraffin-embedded human colon using Hsp47 Rabbit mAb (NBP3-15430) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Hsp47-Antibody-3K3H1-Immunohistochemistry-Paraffin-NBP3-15430-img0001.jpg)
![Western Blot: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - analysis of Human Epithelial cell (A431) lysates showing detection of 47 kDa Hsp47 protein using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 . Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:250 - 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 47 kDa.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1C4-1A6-Western-Blot-NBP2-22400-img0001.jpg "Western Blot: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - analysis of Human Epithelial cell (A431) lysates showing detection of 47 kDa Hsp47 protein using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 . Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:250 - 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 47 kDa.")
![Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1C4-1A6-Immunocytochemistry-Immunofluorescence-NBP2-22400-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x.](http://images.novusbio.com/fullsize/Hsp47-Antibody-1C4-1A6-Immunocytochemistry-Immunofluorescence-NBP2-22400-img0003.jpg)
![Western Blot: Hsp47 Antibody (SR1796) [NBP3-21700] - Western blot analysis using NBP3-21700 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-21700_rabbit-hsp47-mab-sr1796-57202317161867.jpg "Western Blot: Hsp47 Antibody (SR1796) [NBP3-21700] - Western blot analysis using NBP3-21700 in HeLa cell lysate.")
![ELISA: Rat Hsp47 ELISA Kit (Colorimetric) [NBP2-76581] - Samples were spiked with high concentrations of Rat Hsp47 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76581_rat-hsp47-elisa-kit-colorimetric-132202416133651.jpg "ELISA: Rat Hsp47 ELISA Kit (Colorimetric) [NBP2-76581] - Samples were spiked with high concentrations of Rat Hsp47 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Hsp47 ELISA Kit (Colorimetric) [NBP2-76581] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76581_rat-hsp47-elisa-kit-colorimetric-4320248582591.jpg)
![Western Blot: Hsp47 Overexpression Lysate (Adult Normal) [NBL1-15857] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hsp47.](http://images.novusbio.com/fullsize/Hsp47-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15857-img0002.jpg "Western Blot: Hsp47 Overexpression Lysate (Adult Normal) [NBL1-15857] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hsp47.")
![Immunohistochemistry: Hsp47 Antibody (10B2) [NBP3-15163] - IHC image of Hsp47 antibody diluted at 1:100 and staining in paraffin-embedded human placenta tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Hsp47-Antibody-10B2-Immunohistochemistry-NBP3-15163-img0001.jpg "Immunohistochemistry: Hsp47 Antibody (10B2) [NBP3-15163] - IHC image of Hsp47 antibody diluted at 1:100 and staining in paraffin-embedded human placenta tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")