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![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Analysis in human lymph node and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Analysis in human lymph node and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Septin-9 Antibody [NBP2-13294] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1, using anti-SEPT9 antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Western-Blot-NBP2-13294-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-9 Antibody [NBP2-13294] - Staining of human cell line A-431 shows localization to actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13294-img0006.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human Fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0015.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0009.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0010.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human lymph node shows strong cytoplasmic positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0011.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP2-13294] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP2-13294-img0013.jpg)
![Western Blot: Septin-9 Antibody [NBP1-28711] - Lane 1: marker. Lane 2: 10mg of HeLa whole cell lysate probed with NBP1-28711 antibody 1:1000 in PBST. Blot blocked with 5% milk in PBS with 0.1% tween 20 probed overnight at 4C with NBP1-28711 at 1:1000 washed 3X PBST incubated with anti rabbit HRP secondary antiboy, washed 3X PBST, developed with ECL prime kit GE healthcare. Image from verified customer review.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Western-Blot-NBP1-28711-img0003.jpg "Western Blot: Septin-9 Antibody [NBP1-28711] - Lane 1: marker. Lane 2: 10mg of HeLa whole cell lysate probed with NBP1-28711 antibody 1:1000 in PBST. Blot blocked with 5% milk in PBS with 0.1% tween 20 probed overnight at 4C with NBP1-28711 at 1:1000 washed 3X PBST incubated with anti rabbit HRP secondary antiboy, washed 3X PBST, developed with ECL prime kit GE healthcare. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: Septin-9 Antibody [NBP1-28711] - HeLa cells fixed with PFA (4%, 20 min, RT) with anti septin 9 antibody NBP1-28711 at 1:1000. Left panel: Control localisation to sub nuclear filaments. Right panel: Cells treated with cytochalasin D to depolymerize F-actin. Note localization to ring like structures. Image from verified customer review.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-28711-img0004.jpg)
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP1-28711] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Septin 9 used at a dilution of 1:1,000 (1ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-Paraffin-NBP1-28711-img0005.jpg)
![Immunoprecipitation: Septin-9 Antibody [NBP1-28711] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Septin 9 antibody NBP1-28711 used for IP at 3 ug/mg lysate. Septin 9 was also immunoprecipitated by rabbit anti-Septin 9 antibody NBP1-28764, which recognizes an upstream epitope. For blotting immunoprecipitated Septin 9, NBP1-28764 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunoprecipitation-NBP1-28711-img0001.jpg)
![ELISA: Septin-9 Antibody (2C6) [H00010801-M01] - Detection limit for recombinant GST tagged SEPT9 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Septin-9-Antibody-2C6-ELISA-H00010801-M01-img0003.jpg "ELISA: Septin-9 Antibody (2C6) [H00010801-M01] - Detection limit for recombinant GST tagged SEPT9 is 0.03 ng/ml as a capture antibody.")
![Western Blot: Septin-9 Antibody [NBP1-28764] - Hela whole cell lysate (5, 15 and 50ug) probed with NBP1-28764 at 0.04ug/ml.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Western-Blot-NBP1-28764-img0001.jpg "Western Blot: Septin-9 Antibody [NBP1-28764] - Hela whole cell lysate (5, 15 and 50ug) probed with NBP1-28764 at 0.04ug/ml.")
![Immunohistochemistry-Paraffin: Septin-9 Antibody [NBP1-28764] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Septin 9 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunohistochemistry-NBP1-28764-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Septin-9 Antibody [NBP1-28764] - IP of 1mg of Hela whole cell lysate, 20% of IP loaded. Lane 1, IP using NBP1-28764. Lane 2, IP using NBP1-28711 and lane 3, IP using control IgG. Western blot probed with NBP1-28764 at 0.4ug/ml.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunoprecipitation-NBP1-28764-img0002.jpg)
![Western Blot: Septin-9 Antibody [NBP2-20303] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Western-Blot-NBP2-20303-img0002.jpg "Western Blot: Septin-9 Antibody [NBP2-20303] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Septin-9 Antibody [NBP2-20303] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Western-Blot-NBP2-20303-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-9 Antibody [NBP2-55358] - Staining of human cell line U-2 OS shows localization to actin filaments.](http://images.novusbio.com/fullsize/Septin-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55358-img0001.jpg "Immunocytochemistry/Immunofluorescence: Septin-9 Antibody [NBP2-55358] - Staining of human cell line U-2 OS shows localization to actin filaments.")
![Western Blot: Septin-9 Overexpression Lysate (Adult Normal) [NBL1-07150] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-9.](http://images.novusbio.com/fullsize/Septin-9-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07150-img0002.jpg "Western Blot: Septin-9 Overexpression Lysate (Adult Normal) [NBL1-07150] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-9.")