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![Western Blot: SEC14L2 Antibody (4H2) [NBP2-01346] Analysis of extracts (35ug) from 9 different cell lines by using anti-SEC14L2 monoclonal antibody.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Western-Blot-NBP2-01346-img0006.jpg "Western Blot: SEC14L2 Antibody (4H2) [NBP2-01346] Analysis of extracts (35ug) from 9 different cell lines by using anti-SEC14L2 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody (4H2) [NBP2-01346] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SEC14L2.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Immunocytochemistry-Immunofluorescence-NBP2-01346-img0005.jpg)
![Immunohistochemistry-Paraffin: SEC14L2 Antibody (4H2) [NBP2-01346] - Staining of paraffin-embedded Human prostate tissue using anti-SEC14L2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Immunohistochemistry-Paraffin-NBP2-01346-img0004.jpg)
![Flow Cytometry: SEC14L2 Antibody (4H2) [NBP2-01346] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SEC14L2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Flow-Cytometry-NBP2-01346-img0001.jpg)
![Western Blot: SEC14L2 Antibody (4H2) [NBP2-01346] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEC14L2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEC14L2.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Western-Blot-NBP2-01346-img0002.jpg)
![Immunohistochemistry-Paraffin: SEC14L2 Antibody (4H2) [NBP2-01346] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SEC14L2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-4H2-Immunohistochemistry-Paraffin-NBP2-01346-img0003.jpg)
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415133421.jpg "Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415121312.jpg)
![Immunohistochemistry: SEC14L2 Antibody [NBP3-35155] - Immunohistochemistry analysis of paraffin-embedded Rat lung using SEC14L2 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202414201353.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody [NBP3-35155] - Immunofluorescence analysis of U-2 OS cells using SEC14L2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415175267.jpg)
![Western Blot: SEC14L2 Antibody [NBP3-35155] - Western blot analysis of various lysates using SEC14L2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35155_rabbit-sec14l2-pab-912202415152860.jpg)
![Western Blot: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Western blot analysis of SEC14L2 on different lysates with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 16 seconds. 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32935) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-288202412442940.jpg "Western Blot: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Western blot analysis of SEC14L2 on different lysates with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 16 seconds. 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32935) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-298202414262627.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Immunocytochemistry analysis of A549 cells labeling SEC14L2 with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-29202410234211.jpg)
![Western Blot: SEC14L2 Antibody [NBP2-15112] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Western-Blot-NBP2-15112-img0002.jpg "Western Blot: SEC14L2 Antibody [NBP2-15112] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE, antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody [NBP2-15112] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SEC14L2 protein stained by SEC14L2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15112-img0003.jpg)
![Immunohistochemistry-Paraffin: SEC14L2 Antibody [NBP2-15112] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunohistochemistry-Paraffin-NBP2-15112-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody [NBP2-39029] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-39029-img0002.jpg "Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody [NBP2-39029] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SEC14L2 Antibody [NBP2-39029] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunohistochemistry-Paraffin-NBP2-39029-img0004.jpg)
![Immunohistochemistry-Paraffin: SEC14L2 Antibody [NBP2-39029] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunohistochemistry-Paraffin-NBP2-39029-img0003.jpg)
![Western Blot: SEC14L2 Antibody (SR1665) [NBP3-22619] - Western blot analysis using NBP3-22619 in U87-MG cell lysate.](http://images.novusbio.com/fullsize/nbp3-22619_rabbit-sec14l2-mab-sr1665-6720239533748.jpg "Western Blot: SEC14L2 Antibody (SR1665) [NBP3-22619] - Western blot analysis using NBP3-22619 in U87-MG cell lysate.")
![Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody (2E5) [H00023541-M05] - Analysis of monoclonal antibody to SEC14L2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/SEC14L2-Antibody-2E5-Immunocytochemistry-Immunofluorescence-H00023541-M05-img0001.jpg "Immunocytochemistry/Immunofluorescence: SEC14L2 Antibody (2E5) [H00023541-M05] - Analysis of monoclonal antibody to SEC14L2 on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: SEC14L2 Antibody (2E5) [H00023541-M05] - Detection limit for recombinant GST tagged SEC14L2 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-2E5-Sandwich-ELISA-H00023541-M05-img0002.jpg)
![SDS-Page: Recombinant Human SEC14L2 Protein [H00023541-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00023541-P01-1.jpg "SDS-Page: Recombinant Human SEC14L2 Protein [H00023541-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: SEC14L2 Antibody [H00023541-B01P] - Analysis of SEC14L2 expression in human liver.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Western-Blot-H00023541-B01P-img0003.jpg "Western Blot: SEC14L2 Antibody [H00023541-B01P] - Analysis of SEC14L2 expression in human liver.")
![Western Blot: SEC14L2 Antibody [H00023541-B01P] - Analysis of SEC14L2 expression in transfected 293T cell line by SEC14L2 polyclonal antibody. Lane 1: SEC14L2 transfected lysate(43.12 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Western-Blot-H00023541-B01P-img0004.jpg)
![Western Blot: SEC14L2 Antibody [NBP2-15111] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Western-Blot-NBP2-15111-img0001.jpg "Western Blot: SEC14L2 Antibody [NBP2-15111] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: SEC14L2 Overexpression Lysate (Adult Normal) [NBP2-07443] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEC14L2.](http://images.novusbio.com/fullsize/SEC14L2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07443-img0001.jpg "Western Blot: SEC14L2 Overexpression Lysate (Adult Normal) [NBP2-07443] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEC14L2.")