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![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody (M01), clone 3H8.Lane 1: PTBP1 transfected lysate(59.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0019.jpg "Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody (M01), clone 3H8.Lane 1: PTBP1 transfected lysate(59.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of monoclonal antibody to PTBP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Immunocytochemistry-Immunofluorescence-H00005725-M01-img0013.jpg)
![Immunohistochemistry-Paraffin: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of monoclonal antibody to PTBP1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Immunohistochemistry-Paraffin-H00005725-M01-img0015.jpg)
![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - 2 minute exposure for Mouse embryonic stem cell - V6.5 with a dilution of 1:500 - Courtesy of Paul Fields at MIT](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0008.jpg)
![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - 5 minute exposure for Mouse embryonic stem cell - V6.5 with a dilution of 1:500 - Courtesy of Paul Fields at MIT](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0009.jpg)
![Western Blot: PTBP1 Antibody (3H8) [H00005725-M01] - PTBP1 monoclonal antibody (M01), clone 3H8 Analysis of PTBP1 expression in A-431.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Western-Blot-H00005725-M01-img0018.jpg)
![Immunoprecipitation: PTBP1 Antibody (3H8) [H00005725-M01] - Analysis of PTBP1 transfected lysate using anti-PTBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTBP1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-Immunoprecipitation-H00005725-M01-img0016.jpg)
![ELISA: PTBP1 Antibody (3H8) [H00005725-M01] - Detection limit for recombinant GST tagged PTBP1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PTBP1-Antibody-3H8-ELISA-H00005725-M01-img0020.jpg)
![Western Blot: PTBP1 Antibody [NB100-1310] - (0.03ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NB100-1310-img0009.jpg "Western Blot: PTBP1 Antibody [NB100-1310] - (0.03ug/ml) staining of lysates of cell line NIH3T3 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PTBP1 Antibody [NB100-1310] - (2ug/ml) Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Immunohistochemistry-Paraffin-NB100-1310-img0006.jpg)
![Western Blot: PTBP1 Antibody [NB100-1310] - (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NB100-1310-img0007.jpg)
![Western Blot: PTBP1 Antibody [NB100-1310] - (0.01ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NB100-1310-img0008.jpg)
![Western Blot: PTBP1 Antibody [NB100-1310] - (0.1-0.3ug/ml) staining of Mouse Ovary (A) and Rat Ovary (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NB100-1310-img0010.jpg)
![Western Blot: PTBP1 Antibody (9M6A8) [NBP3-16197] - Western blot analysis of extracts of various cell lines, using PTBP1 Rabbit mAb (NBP3-16197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PTBP1-Antibody-9M6A8-Western-Blot-NBP3-16197-img0004.jpg "Western Blot: PTBP1 Antibody (9M6A8) [NBP3-16197] - Western blot analysis of extracts of various cell lines, using PTBP1 Rabbit mAb (NBP3-16197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: PTBP1 Antibody (9M6A8) [NBP3-16197] - Immunofluorescence analysis of U-2 OS cells using PTBP1 Rabbit mAb (NBP3-16197) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTBP1-Antibody-9M6A8-Immunocytochemistry-Immunofluorescence-NBP3-16197-img0003.jpg)
![Immunohistochemistry-Paraffin: PTBP1 Antibody (9M6A8) [NBP3-16197] - Immunohistochemistry of paraffin-embedded human colon carcinoma using PTBP1 Rabbit mAb (NBP3-16197) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PTBP1-Antibody-9M6A8-Immunohistochemistry-Paraffin-NBP3-16197-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PTBP1 Antibody (9M6A8) [NBP3-16197] - Immunofluorescence analysis of NIH-3T3 cells using PTBP1 Rabbit mAb (NBP3-16197) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PTBP1-Antibody-9M6A8-Immunocytochemistry-Immunofluorescence-NBP3-16197-img0002.jpg)
![Western Blot: PTBP1 Antibody (5C1-R) [NBP3-32870] - Western blot analysis of PTBP1 on different lysates with Mouse anti-PTBP1 antibody (NBP3-32870) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: SW620 cell lysate (20 ug/Lane) Lane 7: A431 cell lysate (20 ug/Lane) Lane 8: A549 cell lysate (20 ug/Lane) Lane 9: NIH/3T3 cell lysate (20 ug/Lane) Lane 10: C6 cell lysate (20 ug/Lane) Lane 11: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 60 kDa Observed band size: 55 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32870) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32870_mouse-ptbp1-mab-5c1-r-288202412461666.jpg "Western Blot: PTBP1 Antibody (5C1-R) [NBP3-32870] - Western blot analysis of PTBP1 on different lysates with Mouse anti-PTBP1 antibody (NBP3-32870) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: SW620 cell lysate (20 ug/Lane) Lane 7: A431 cell lysate (20 ug/Lane) Lane 8: A549 cell lysate (20 ug/Lane) Lane 9: NIH/3T3 cell lysate (20 ug/Lane) Lane 10: C6 cell lysate (20 ug/Lane) Lane 11: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 60 kDa Observed band size: 55 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32870) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTBP1 Antibody (5C1-R) [NBP3-32870] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-PTBP1 antibody (NBP3-32870) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32870) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32870_mouse-ptbp1-mab-5c1-r-29820241492634.jpg)
![Immunocytochemistry/ Immunofluorescence: PTBP1 Antibody (5C1-R) [NBP3-32870] - Immunocytochemistry analysis of HeLa cells labeling PTBP1 with Mouse anti-PTBP1 antibody (NBP3-32870) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PTBP1 antibody (NBP3-32870) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32870_mouse-ptbp1-mab-5c1-r-2920241114831.jpg)
![Western Blot: PTBP1 Antibody [NBP2-19996] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTBP1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NBP2-19996-img0007.jpg "Western Blot: PTBP1 Antibody [NBP2-19996] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTBP1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PTBP1 Antibody [NBP2-19996] - Mouse brain. PTBP1 stained by PTBP1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-19996-img0008.jpg)
![Western Blot: PTBP1 Antibody [NBP2-19996] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTBP1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NBP2-19996-img0005.jpg)
![Immunohistochemistry-Paraffin: PTBP1 Antibody [NBP2-19996] - AGS xenograft, using hnRNP 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Immunohistochemistry-Paraffin-NBP2-19996-img0003.jpg)
![Immunohistochemistry: PTBP1 Antibody (8A1) [NBP3-26363] - Image of PTBP1 Antibody (8A1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26363_rabbit-ptbp1-mab-8a1-262202415355729.jpg "Immunohistochemistry: PTBP1 Antibody (8A1) [NBP3-26363] - Image of PTBP1 Antibody (8A1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PTBP1 Antibody (8A1) [NBP3-26363] - Image of PTBP1 Antibody (8A1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26363_rabbit-ptbp1-mab-8a1-2622024152244.jpg)
![Western Blot: PTBP1 Antibody (8A1) [NBP3-26363] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate. All lanes: PTBP1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 60, 60 kDa Observed band size: 58 kDa](http://images.novusbio.com/fullsize/nbp3-26363_rabbit-ptbp1-mab-8a1-2822024115252.jpg)
![Western Blot: PTBP1 Antibody (S01-1G2) [NBP3-19910] - Western blot detection of PTBP1 in Hela, CHO-K1, Ramos using NBP3-19910 (1:1000 diluted)](http://images.novusbio.com/fullsize/PTBP1-Antibody-S01-1G2-Western-Blot-NBP3-19910-img0002.jpg "Western Blot: PTBP1 Antibody (S01-1G2) [NBP3-19910] - Western blot detection of PTBP1 in Hela, CHO-K1, Ramos using NBP3-19910 (1:1000 diluted)")
![Immunohistochemistry-Paraffin: PTBP1 Antibody (S01-1G2) [NBP3-19910] - Immunohistochemistry of PTBP1 in paraffin-embedded Human tonsil using NBP3-19910 at dilution 1/50](http://images.novusbio.com/fullsize/PTBP1-Antibody-S01-1G2-Immunohistochemistry-Paraffin-NBP3-19910-img0001.jpg)
![Knockout Validated: PTBP1 Antibody [NBP3-02956] - Analysis of extracts from normal (control) and PTBP1 knockout (KO) HeLa cells, using PTBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 3min.](http://images.novusbio.com/fullsize/PTBP1-Antibody-Knockout-Validated-NBP3-02956-img0001.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: PTBP1 Antibody [NBP3-02956] - Analysis of extracts from normal (control) and PTBP1 knockout (KO) HeLa cells, using PTBP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 3min.")
![Western Blot: PTBP1 Antibody [NBP1-57123] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NBP1-57123-img0003.jpg "Western Blot: PTBP1 Antibody [NBP1-57123] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml")
![Western Blot: PTBP1 Antibody [NBP1-57123] - Reccomended Titration: 0.2 - 1 ug/ml Positive Control: Daudi cell lysate PTBP1 is strongly supported by BioGPS gene expression data to be expressed in Human Daudi cells](http://images.novusbio.com/fullsize/PTBP1-Antibody-Western-Blot-NBP1-57123-img0004.jpg)
![SDS-Page: Recombinant Human PTBP1 Protein [H00005725-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005725-P02-1.jpg "SDS-Page: Recombinant Human PTBP1 Protein [H00005725-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human PTBP1 Protein [H00005725-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005725-Q01-1.jpg "SDS-Page: Recombinant Human PTBP1 Protein [H00005725-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")