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![Immunocytochemistry/Immunofluorescence: Prox1 Antibody (5G10) [NBP1-30045] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PROX1 (5G10) NBP1-30045 at a 1:200 dilution overnight at 4C and detected with and anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Prox1-Antibody-5G10-Immunocytochemistry-Immunofluorescence-NBP1-30045-img0002.jpg "Immunocytochemistry/Immunofluorescence: Prox1 Antibody (5G10) [NBP1-30045] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PROX1 (5G10) NBP1-30045 at a 1:200 dilution overnight at 4C and detected with and anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry: Prox1 Antibody (5G10) [NBP1-30045] - Rat dentate gyrus showing specific immunolabeling of the prox1 protein. Photo courtesy of Justin Kievits and Teresa Milner, Weill Cornell Medical College.](http://images.novusbio.com/fullsize/Prox1-Antibody-5G10-Immunohistochemistry-NBP1-30045-img0001.jpg)
![Flow Cytometry: Prox1 Antibody (5G10) [NBP1-30045] - An intracellular stain was performed on HepG2 cells with Prox1 [5G10] Antibody NBP1-30045AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Prox1-Antibody-5G10-Flow-Cytometry-NBP1-30045-img0007.jpg)
![Immunohistochemistry-Paraffin: Prox1 Antibody (5G10) [NBP1-30045] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot UD1009o of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic staining in sinusoidal endothelial cells as well as in Kupffer cells.](http://images.novusbio.com/fullsize/Prox1-Antibody-5G10-Immunohistochemistry-Paraffin-NBP1-30045-img0004.jpg)
![Immunohistochemistry-Paraffin: Prox1 Antibody (5G10) [NBP1-30045] - IHC analysis of a formalin fixed paraffin embedded tissue section of Mouse liver using Lot A of PROX1 antibody clone 5G10 at 1:100 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice nuclear-cytoplasmic staining in sinusoidal endothelial cells as well as in Kupffer cells.](http://images.novusbio.com/fullsize/Prox1-Antibody-5G10-Immunohistochemistry-Paraffin-NBP1-30045-img0005.jpg)
![Flow Cytometry: Prox1 Antibody (5G10) [NBP1-30045] - HepG2 cells were stained with Prox1 (5G10) NBP1-30045 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/Prox1-Antibody-5G10-Flow-Cytometry-NBP1-30045-img0006.jpg)
![Western Blot: Prox1 Antibody [NBP1-18605] - Analysis with anti-human Prox-1](http://images.novusbio.com/fullsize/Prox1-Antibody-Western-Blot-NBP1-18605-img0007.jpg "Western Blot: Prox1 Antibody [NBP1-18605] - Analysis with anti-human Prox-1")
![Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18605] - Cells expressing LLEC markers are present within embryonic mouse leptomeninges. As revealed by IHC, E12.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (d). Scale?=?30 um. n?=?3 brains. E13.5 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). DAPI marks the nuclei in blue (h). Scale?=?30 um. n?=?3 brains. E18 mouse meninges (white bracket) contain cells that co-express LYVE1 (red), PROX1 (green), and MRC1 (white). PROX1 is cytoplasmic ( red arrows) in the meninges but nuclear in neurons ( blue arrows). DAPI marks the nuclei in blue. Image collected and cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00401-019-02091-z) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Prox1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18605-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18605] - PROX1 Antibody [NBP1-18605] - human skin stained with PROX1 (red), VEGFR3 (green) and TO-PRO (nuclei). ICC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Prox1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18605-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Prox1 Antibody [NBP1-18605] - Mouse LLECs develop independent of the transcription factor PU.1. a–d As detected by IHC, E15.5 wild-type mice have cells that co-express LYVE1 (a, red) PROX1 (b, green) & MRC1 (c, white) in the developing meninges (white bracket). DAPI labels nuclei blue in the merged image (d). White arrows indicate cells with these three markers. Scale = 50 µm. n = 2 brains. e–h) The meninges (white bracket) of E15.5 PU.1 knockout siblings contain many cells (white arrows) that co-express LYVE1 (e, red), PROX1 (f, green), & MRC1 (g, white). Scale = 50 µm. n = 3 brains Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-18605_rabbit-polyclonal-prox1-antibody-310202415293336.jpg)
![Western Blot: Prox1 Antibody [NBP1-18606] - Western Blot analysis with Prox-1 Antibody.](http://images.novusbio.com/fullsize/Prox1-Antibody-Western-Blot-NBP1-18606-img0019.jpg "Western Blot: Prox1 Antibody [NBP1-18606] - Western Blot analysis with Prox-1 Antibody.")
![Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18606] - Double IF stanining of human Prox-1 and CD31 in a mix of HUVEC and HDLEC with Prox1 Antibody (green).](http://images.novusbio.com/fullsize/Prox1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18606-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP1-18606] - Immunofluorescence staining (green) of primary human dermal lymphatic endothelial cells (HDLEC) with anti-human Prox-1 (1ug/ml) and counter staining of nuclei with Dapi. As secondary antibody goat anti-rabbit ALEXA Flour 488 was used 1:400](http://images.novusbio.com/fullsize/Prox1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18606-img0017.jpg)
![Western Blot: Prox1 Antibody (9I9A7) [NBP3-16739] - Western blot analysis of extracts of various cell lines, using Prox1 Rabbit mAb (NBP3-16739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Prox1-Antibody-9I9A7-Western-Blot-NBP3-16739-img0002.jpg "Western Blot: Prox1 Antibody (9I9A7) [NBP3-16739] - Western blot analysis of extracts of various cell lines, using Prox1 Rabbit mAb (NBP3-16739) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunohistochemistry-Paraffin: Prox1 Antibody (9I9A7) [NBP3-16739] - Immunohistochemistry of paraffin-embedded human placenta using Prox1 Rabbit mAb (NBP3-16739) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Prox1-Antibody-9I9A7-Immunohistochemistry-Paraffin-NBP3-16739-img0001.jpg)
![Western Blot: Prox1 Antibody (S02-8H5) [NBP3-19747] - Western blot detection of Prox1 in C6 cell lysates using NBP3-19747 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 85kDa.](http://images.novusbio.com/fullsize/Prox1-Antibody-S02-8H5-Western-Blot-NBP3-19747-img0001.jpg "Western Blot: Prox1 Antibody (S02-8H5) [NBP3-19747] - Western blot detection of Prox1 in C6 cell lysates using NBP3-19747 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 85kDa.")
![Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP2-55154] - Staining of human cell line Hep G2 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Prox1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55154-img0001.jpg "Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP2-55154] - Staining of human cell line Hep G2 shows localization to nucleoplasm.")
![Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP2-55222] - Staining of human cell line Hep G2 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Prox1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55222-img0001.jpg "Immunocytochemistry/Immunofluorescence: Prox1 Antibody [NBP2-55222] - Staining of human cell line Hep G2 shows localization to nucleoplasm.")
![Western Blot: PROX1 Overexpression Lysate (Adult Normal) [NBL1-14814] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PROX1.](http://images.novusbio.com/fullsize/PROX1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14814-img0002.jpg "Western Blot: PROX1 Overexpression Lysate (Adult Normal) [NBL1-14814] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PROX1.")