| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3489-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3489-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20102-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20103-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03275-20ul | |||
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX2 monoclonal antibody (M01), clone 4E10-2D2 Analysis of PRDX2 expression in Hela.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-4E10-2D2-Western-Blot-H00007001-M01-img0011.jpg "Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX2 monoclonal antibody (M01), clone 4E10-2D2 Analysis of PRDX2 expression in Hela.")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Analysis of monoclonal antibody to PRDX2 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-4E10-2D2-Immunocytochemistry-Immunofluorescence-H00007001-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Analysis of monoclonal antibody to PRDX2 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-4E10-2D2-Immunohistochemistry-Paraffin-H00007001-M01-img0009.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Analysis of PRDX2 expression in transfected 293T cell line by PRDX2 monoclonal antibody (M01), clone 4E10-2D2.Lane 1: PRDX2 transfected lysate(21.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-4E10-2D2-Western-Blot-H00007001-M01-img0012.jpg)
![ELISA: Peroxiredoxin 2 Antibody (4E1.0-2D2) [H00007001-M01] - Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Detection limit for recombinant GST tagged PRDX2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-4E1-0-2D2-ELISA-H00007001-M01-img0013.jpg)
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Knockdown Validated: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX2 knockdown increases PRDX4 protein levels in HeLa cells. HeLa subclones were exposed to 20% or 1% O2 for indicated time. Each WCL was subject to immunoblot assays with the indicated antibodies (PRDX2 H00007001-M01 and PRDX4 NBP1-31435). Image collected and cropped by CiteAb from the following publication (oncotarget.com/abstract/7142), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-4E10-2D2-Knockdown-Validated-H00007001-M01-img0014.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Expression of PRDX2 or PRDX4 does not affect HIF-1 alpha or HIF-2 alpha protein levelsA. & B. HeLa cells were transfected with EV or vector encoding PRDX2-V5 (A, P2) or PRDX4-V5 (B, P4), & exposed to 20% or 1% O2 for 24 h. WCL was subject to immunoblot assays with antibody against HIF-1 alpha , HIF-2 alpha , V5, or acin. C. HeLa-shSC (sc) & HeLa-shPRDX(2+4) (2+4) cells were exposed to 20% or 1% O2 for 24 h in the presence of doxycycline. WCL was subject to immunoblot assays with antibodies against HIF-1 alpha , HIF-2 alpha , PRDX2, PRDX4, & actin. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007001-m01_mouse-monoclonal-peroxiredoxin-2-antibody-4e10-2d2-31020241534360.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX2 knockdown increases PRDX4 protein levels in HeLa cellsHeLa subclones were exposed to 20% or 1% O2 for indicated time. Each WCL was subject to immunoblot assays with the indicated antibodies. SC, scrambled control shRNA. P2, PRDX2 shRNA. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007001-m01_mouse-monoclonal-peroxiredoxin-2-antibody-4e10-2d2-310202415304247.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Hypoxia induces the nuclear translocation of PRDX2 & PRDX4HeLa cells were transfected with vector encoding PRDX2-V5 (P2) or PRDX4-V5 (P4), or empty vector (EV), & exposed to 20% or 1% O2 for 48 h. Nuclear & cytosolic fractions were isolated & subject to immunoblot assays with antibodies against HIF-1 alpha , HIF-2 alpha , V5, alpha -tubulin, & histone H3. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007001-m01_mouse-monoclonal-peroxiredoxin-2-antibody-4e10-2d2-310202415304252.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Mapping the PRDX2 & PRDX4 binding domains of HIF-1 alpha A. & B. HeLa cells were transfected with PRDX2-V5 (A) or PRDX4-V5 (B) vector & WCL was incubated with purified GST or GST-HIF-1 alpha fusion protein in the presence of glutathione-Sepharose beads, followed by immunoblot assays with anti-V5 antibody (upper panels) or Ponceau S staining (lower panels). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007001-m01_mouse-monoclonal-peroxiredoxin-2-antibody-4e10-2d2-31020241534389.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Expression of PRDX2 or PRDX4 does not affect HIF-1 alpha or HIF-2 alpha protein levelsA. & B. HeLa cells were transfected with EV or vector encoding PRDX2-V5 (A, P2) or PRDX4-V5 (B, P4), & exposed to 20% or 1% O2 for 24 h. WCL was subject to immunoblot assays with antibody against HIF-1 alpha , HIF-2 alpha , V5, or acin. C. HeLa-shSC (sc) & HeLa-shPRDX(2+4) (2+4) cells were exposed to 20% or 1% O2 for 24 h in the presence of doxycycline. WCL was subject to immunoblot assays with antibodies against HIF-1 alpha , HIF-2 alpha , PRDX2, PRDX4, & actin. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007001-m01_mouse-monoclonal-peroxiredoxin-2-antibody-4e10-2d2-310202415175220.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - Effect of PRDX2 & PRDX4 on HIF-1 alpha -p300 interactionHeLa cells were transfected with empty vector (EV) or vector encoding PRDX2-V5 or PRDX4-V5, & exposed to 1% O2 for 24 h. WCL was subject to IP with anti-p300 antibody, followed by immunoblot assays using antibodies against HIF-1 alpha , V5, & p300. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007001-m01_mouse-monoclonal-peroxiredoxin-2-antibody-4e10-2d2-310202415392540.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX2 expression is regulated by HIF-1 & HIF-2A. HeLa cells were exposed to 20% or 1% O2 for 24 h. RT-qPCR assays were performed using primers specific for the indicated mRNAs. Data are shown as mean ± SEM, n = 3. ***p < 0.001 versus 20% O2. B. HeLa cells were exposed to 20% or 1% O2 for the indicated time. WCLs were subject to immunoblot assays with antibodies against PRDX2, HIF-1 alpha , HIF-2 alpha , & actin. The PRDX2 & actin bands were quantified by densitometry & normalized to 0 h (20% O2). Normalized data are shown as mean ± SEM, n = 3. *p < 0.05, ***p < 0.001 versus 20% O2. C. HeLa-shSC (SC), HeLa-shHIF-1 alpha (1 alpha ), HeLa-shHIF-2 alpha (2 alpha ), & HeLa-sh1 alpha +2 alpha (DKD) cells were exposed to 20% or 1% O2 for 72 h. WCLs were subject to immunoblot assays with antibodies against PRDX2, HIF-1 alpha , HIF-2 alpha , & actin. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007001-m01_mouse-monoclonal-peroxiredoxin-2-antibody-4e10-2d2-310202415482276.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX proteins bind to HIF-1 alpha & HIF-2 alpha A. HeLa cells were transfected with an expression vector encoding V5-epitope-tagged PRDX2 (PRDX2-V5) & exposed to 1% O2 for 24 h. Whole cell lysate (WCL) was subject to immunoprecipitation (IP) using anti-HIF-1 alpha antibody or control IgG, followed by immunoblot assays with antibody against V5 epitope or HIF-1 alpha . B. HeLa cells were transfected with PRDX2-V5 vector & exposed to 1% O2 for 24 h. The WCL was subject to IP using anti-V5 antibody or control IgG, followed by immunoblot assays with antibody against V5 or HIF-1 alpha . Light IgG: immunoglobulin light chain from the secondary antibody. C. HeLa cells were transfected with vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-1 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-1 alpha . D. HeLa cells were transfected with empty vector (EV) or vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-2 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-2 alpha . Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007001-m01_mouse-monoclonal-peroxiredoxin-2-antibody-4e10-2d2-310202415482298.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX proteins bind to HIF-1 alpha & HIF-2 alpha A. HeLa cells were transfected with an expression vector encoding V5-epitope-tagged PRDX2 (PRDX2-V5) & exposed to 1% O2 for 24 h. Whole cell lysate (WCL) was subject to immunoprecipitation (IP) using anti-HIF-1 alpha antibody or control IgG, followed by immunoblot assays with antibody against V5 epitope or HIF-1 alpha . B. HeLa cells were transfected with PRDX2-V5 vector & exposed to 1% O2 for 24 h. The WCL was subject to IP using anti-V5 antibody or control IgG, followed by immunoblot assays with antibody against V5 or HIF-1 alpha . Light IgG: immunoglobulin light chain from the secondary antibody. C. HeLa cells were transfected with vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-1 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-1 alpha . D. HeLa cells were transfected with empty vector (EV) or vector encoding a V5-tagged PRDX family member & exposed to 1% O2 for 24 h. WCL was subject to IP using anti-HIF-2 alpha antibody, followed by immunoblot assays with antibody against V5 or HIF-2 alpha . Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007001-m01_mouse-monoclonal-peroxiredoxin-2-antibody-4e10-2d2-310202415485134.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (4E10-2D2) [H00007001-M01] - PRDX2 expression is regulated by HIF-1 & HIF-2A. HeLa cells were exposed to 20% or 1% O2 for 24 h. RT-qPCR assays were performed using primers specific for the indicated mRNAs. Data are shown as mean ± SEM, n = 3. ***p < 0.001 versus 20% O2. B. HeLa cells were exposed to 20% or 1% O2 for the indicated time. WCLs were subject to immunoblot assays with antibodies against PRDX2, HIF-1 alpha , HIF-2 alpha , & actin. The PRDX2 & actin bands were quantified by densitometry & normalized to 0 h (20% O2). Normalized data are shown as mean ± SEM, n = 3. *p < 0.05, ***p < 0.001 versus 20% O2. C. HeLa-shSC (SC), HeLa-shHIF-1 alpha (1 alpha ), HeLa-shHIF-2 alpha (2 alpha ), & HeLa-sh1 alpha +2 alpha (DKD) cells were exposed to 20% or 1% O2 for 72 h. WCLs were subject to immunoblot assays with antibodies against PRDX2, HIF-1 alpha , HIF-2 alpha , & actin. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.7142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00007001-m01_mouse-monoclonal-peroxiredoxin-2-antibody-4e10-2d2-31020241548364.jpg)
![N/A Peroxiredoxin 2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Peroxiredoxin_2_DY348905_ELISA_2433.jpg "N/A Peroxiredoxin 2 [Biotin]")
![Western Blot: Peroxiredoxin 2 Antibody [NBP2-19776] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with PRX II antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-Western-Blot-NBP2-19776-img0006.jpg "Western Blot: Peroxiredoxin 2 Antibody [NBP2-19776] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with PRX II antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Frozen: Peroxiredoxin 2 Antibody [NBP2-19776] - Frozen section of rat liver. Peroxiredoxin 2 antibody diluted at 1:200.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-Immunohistochemistry-Frozen-NBP2-19776-img0007.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 2 Antibody [NBP2-19776] - Human breast cancer, using Peroxiredoxin 2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-19776-img0003.jpg)
![SDS-Page: Recombinant Rat Peroxiredoxin 2 Protein [NBP2-52150] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Recombinant-Protein-SDS-Page-NBP2-52150-img0001.jpg "SDS-Page: Recombinant Rat Peroxiredoxin 2 Protein [NBP2-52150] - 15% SDS Page (3 ug)")
![Western Blot: Peroxiredoxin 2 Antibody (SAIC-34B-124) - Azide and BSA Free [NBP3-20102] - HepG2(A) (0.003ug/ml), HeLa(B) (0.003ug/ml), MCF7(C) (0.003ug/ml), K562(D) (0.003ug/ml) and LNCaP (E) (0.001ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-34B-124 (NBP3-20102), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20102_rabbit-peroxiredoxin-2-mab-saic-34b-124-azide-bsa-free-274202396464.png "Western Blot: Peroxiredoxin 2 Antibody (SAIC-34B-124) - Azide and BSA Free [NBP3-20102] - HepG2(A) (0.003ug/ml), HeLa(B) (0.003ug/ml), MCF7(C) (0.003ug/ml), K562(D) (0.003ug/ml) and LNCaP (E) (0.001ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-34B-124 (NBP3-20102), before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Flow Cytometry: Peroxiredoxin 2 Antibody (SAIC-34B-124) - Azide and BSA Free [NBP3-20102] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-34B-124 (NBP3-20102, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20102_rabbit-peroxiredoxin-2-mab-saic-34b-124-azide-bsa-free-2742023924230.png)
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 2 Antibody (SAIC-34B-124) - Azide and BSA Free [NBP3-20102] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-34B-124 (NBP3-20102) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20102, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20102_rabbit-peroxiredoxin-2-mab-saic-34b-124-azide-bsa-free-2742023924259.png)
![Western Blot: Peroxiredoxin 2 Antibody (7F5-R) [NBP3-32851] - Western blot analysis of Peroxiredoxin 2 on different lysates with Mouse anti-Peroxiredoxin 2 antibody (NBP3-32851) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: LNCaP cell lysate Lane 3: HeLa cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: MCF7 cell lysate Lane 6: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32851) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32851_mouse-peroxiredoxin-2-mab-7f5-r-288202412442952.jpg "Western Blot: Peroxiredoxin 2 Antibody (7F5-R) [NBP3-32851] - Western blot analysis of Peroxiredoxin 2 on different lysates with Mouse anti-Peroxiredoxin 2 antibody (NBP3-32851) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: LNCaP cell lysate Lane 3: HeLa cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: MCF7 cell lysate Lane 6: HepG2 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32851) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Peroxiredoxin 2 Antibody (7F5-R) [NBP3-32851] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Peroxiredoxin 2 antibody (NBP3-32851) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32851) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32851_mouse-peroxiredoxin-2-mab-7f5-r-298202414161834.jpg)
![Immunocytochemistry/ Immunofluorescence: Peroxiredoxin 2 Antibody (7F5-R) [NBP3-32851] - Immunocytochemistry analysis of HeLa cells labeling Peroxiredoxin 2 with Mouse anti-Peroxiredoxin 2 antibody (NBP3-32851) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Peroxiredoxin 2 antibody (NBP3-32851) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32851_mouse-peroxiredoxin-2-mab-7f5-r-2920241048484.jpg)
![Western Blot: Peroxiredoxin 2 Antibody [NBP1-54337] - Hela cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-Western-Blot-NBP1-54337-img0001.jpg "Western Blot: Peroxiredoxin 2 Antibody [NBP1-54337] - Hela cell lysate, Antibody Titration: 0.2-1 ug/ml")
![Immunohistochemistry-Paraffin: Peroxiredoxin 2 Antibody [NBP1-54337] - Formalin Fixed Paraffin; Embedded Tissue: Human Pineal Tissue; Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes; Primary Antibody Concentration: 1:100](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54337-img0004.jpg)
![Immunohistochemistry-Paraffin: Peroxiredoxin 2 Antibody [NBP1-54337] - Human Liver tissue, 5 ug/ml.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-54337-img0002.jpg)
![Western Blot: Peroxiredoxin 2 Antibody (S02-1G5) [NBP3-19735] - Western blot detection of Peroxiredoxin 2 in K562 cell lysates using NBP3-19735 (1:1000 diluted). Predicted band size: 22kDa. Observed band size: 22kDa.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-S02-1G5-Western-Blot-NBP3-19735-img0002.jpg "Western Blot: Peroxiredoxin 2 Antibody (S02-1G5) [NBP3-19735] - Western blot detection of Peroxiredoxin 2 in K562 cell lysates using NBP3-19735 (1:1000 diluted). Predicted band size: 22kDa. Observed band size: 22kDa.")
![Immunohistochemistry-Paraffin: Peroxiredoxin 2 Antibody (S02-1G5) [NBP3-19735] - Immunohistochemistry of Peroxiredoxin 2 in paraffin-embedded Human breast cancer tissue using NBP3-19735 at dilution 1/50](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-S02-1G5-Immunohistochemistry-Paraffin-NBP3-19735-img0001.jpg)
![Flow Cytometry: Peroxiredoxin 2 Antibody (SAIC-34D-11) - Azide and BSA Free [NBP3-20103] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-34D-11 (NBP3-20103, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20103_rabbit-peroxiredoxin-2-mab-saic-34d-11-azide-bsa-free-274202396467.png "Flow Cytometry: Peroxiredoxin 2 Antibody (SAIC-34D-11) - Azide and BSA Free [NBP3-20103] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-34D-11 (NBP3-20103, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry/Immunofluorescence: Peroxiredoxin 2 Antibody (SAIC-34D-11) - Azide and BSA Free [NBP3-20103] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-34D-11 (NBP3-20103) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20103, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20103_rabbit-peroxiredoxin-2-mab-saic-34d-11-azide-bsa-free-2742023924285.png)
![Knockout Validated: Peroxiredoxin 2 Antibody [NBP3-03275] - Analysis of extracts from normal (control) and PRDx2 knockout (KO) 293T cells, using Peroxiredoxin 2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Peroxiredoxin-2-Antibody-Knockout-Validated-NBP3-03275-img0002.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Peroxiredoxin 2 Antibody [NBP3-03275] - Analysis of extracts from normal (control) and PRDx2 knockout (KO) 293T cells, using Peroxiredoxin 2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% 3% nonfat dry milk in TBST.")