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![Western Blot: PQBP1 Antibody [NBP1-82619] - Analysis in control (vector only transfected HEK293T lysate) and PQBP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/PQBP1-Antibody-Western-Blot-NBP1-82619-img0004.jpg "Western Blot: PQBP1 Antibody [NBP1-82619] - Analysis in control (vector only transfected HEK293T lysate) and PQBP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: PQBP1 Antibody [NBP1-82619] - Staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PQBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82619-img0003.jpg)
![Immunohistochemistry-Paraffin: PQBP1 Antibody [NBP1-82619] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells and islet cells.](http://images.novusbio.com/fullsize/PQBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-82619-img0002.jpg)
![Western Blot: PQBP1 Antibody [NBP1-47271] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47271 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47270, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/PQBP1-Antibody-Western-Blot-NBP1-47271-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PQBP1 Antibody [NBP1-47271] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47271 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47270, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: PQBP1 Antibody [NBP1-47271] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 used at a dilution of 1:200 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit](http://images.novusbio.com/fullsize/PQBP1-Antibody-Immunohistochemistry-NBP1-47271-img0003.jpg)
![Western Blot: PQBP1 Antibody [NBP1-47270] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47270 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47271, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/PQBP1-Antibody-Western-Blot-NBP1-47270-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PQBP1 Antibody [NBP1-47270] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47270 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47271, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: PQBP1 Antibody [NBP1-47270] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 used at a dilution of 1:1,000 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit](http://images.novusbio.com/fullsize/PQBP1-Antibody-Immunohistochemistry-NBP1-47270-img0003.jpg)
![Western Blot: PQBP1 Antibody [NBP2-99606] - Anti-PQBP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 38 kDa](http://images.novusbio.com/fullsize/PQBP1-Antibody-Western-Blot-NBP2-99606-img0003.jpg "Western Blot: PQBP1 Antibody [NBP2-99606] - Anti-PQBP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 38 kDa")
![Immunocytochemistry/Immunofluorescence: PQBP1 Antibody [NBP2-99606] - Immunofluorescence staining of PQBP1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human PQBP1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/PQBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99606-img0001.jpg)
![Western Blot: PQBP1 Antibody (1A11) [H00010084-M01] - Analysis of PQBP1 expression in transfected 293T cell line by PQBP1 monoclonal antibody (M01), clone 1A11.Lane 1: PQBP1 transfected lysate(30.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PQBP1-Antibody-1A11-Western-Blot-H00010084-M01-img0002.jpg "Western Blot: PQBP1 Antibody (1A11) [H00010084-M01] - Analysis of PQBP1 expression in transfected 293T cell line by PQBP1 monoclonal antibody (M01), clone 1A11.Lane 1: PQBP1 transfected lysate(30.5 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: PQBP1 Antibody (1A11) [H00010084-M01] - Detection limit for recombinant GST tagged PQBP1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PQBP1-Antibody-1A11-Sandwich-ELISA-H00010084-M01-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PQBP1 Antibody (3H7) [H00010084-M15] - Analysis of monoclonal antibody to PQBP1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PQBP1-Antibody-3H7-Immunocytochemistry-Immunofluorescence-H00010084-M15-img0002.jpg "Immunocytochemistry/Immunofluorescence: PQBP1 Antibody (3H7) [H00010084-M15] - Analysis of monoclonal antibody to PQBP1 on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: PQBP1 Antibody (3H7) [H00010084-M15] - Detection limit for recombinant GST tagged PQBP1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PQBP1-Antibody-3H7-Sandwich-ELISA-H00010084-M15-img0003.jpg)
![Western Blot: PQBP1 Antibody [NBP3-38089] - Western blot analysis of various lysates using PQBP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38089_rabbit-pqbp1-pab-912202415143642.jpg "Western Blot: PQBP1 Antibody [NBP3-38089] - Western blot analysis of various lysates using PQBP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Western Blot: PQBP1 Antibody (8D8H6) [NBP3-16075] - Western blot analysis of extracts of various cell lines, using PQBP1 antibody (NBP3-16075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/PQBP1-Antibody-8D8H6-Western-Blot-NBP3-16075-img0001.jpg "Western Blot: PQBP1 Antibody (8D8H6) [NBP3-16075] - Western blot analysis of extracts of various cell lines, using PQBP1 antibody (NBP3-16075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: PQBP1 Overexpression Lysate (Adult Normal) [NBL1-14717] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PQBP1.](http://images.novusbio.com/fullsize/PQBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14717-img0002.jpg "Western Blot: PQBP1 Overexpression Lysate (Adult Normal) [NBL1-14717] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PQBP1.")
![Western Blot: PQBP1 Overexpression Lysate (Adult Normal) [NBP2-09185] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PQBP1.](http://images.novusbio.com/fullsize/PQBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09185-img0001.jpg "Western Blot: PQBP1 Overexpression Lysate (Adult Normal) [NBP2-09185] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PQBP1.")
![Western Blot: PQBP1 Overexpression Lysate (Adult Normal) [NBP2-09184] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PQBP1.](http://images.novusbio.com/fullsize/PQBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09184-img0001.jpg "Western Blot: PQBP1 Overexpression Lysate (Adult Normal) [NBP2-09184] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PQBP1.")
![Western Blot: PQBP1 Overexpression Lysate (Adult Normal) [NBP2-09183] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PQBP1.](http://images.novusbio.com/fullsize/PQBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09183-img0001.jpg "Western Blot: PQBP1 Overexpression Lysate (Adult Normal) [NBP2-09183] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PQBP1.")