Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: Ndufs4 Antibody [NBP1-89026] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-89026-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Ndufs4 Antibody [NBP1-89026] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody [NBP1-89026] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89026-img0009.jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody [NBP1-89026] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunohistochemistry-Paraffin-NBP1-89026-img0015.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-89026] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-89026-img0008.jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody [NBP1-89026] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunohistochemistry-Paraffin-NBP1-89026-img0012.jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody [NBP1-89026] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunohistochemistry-Paraffin-NBP1-89026-img0013.jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody [NBP1-89026] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunohistochemistry-Paraffin-NBP1-89026-img0014.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample(30ug whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0007.jpg "Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample(30ug whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody [NBP1-31465] - Analysis of methanol-fixed HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31465-img0003.jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody [NBP1-31465] - Human ovarian cancer, using NDUFS4 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Immunohistochemistry-Paraffin-NBP1-31465-img0004.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0005.jpg)
![Western Blot: Ndufs4 Antibody [NBP1-31465] - 50 ug Rat heart lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-Western-Blot-NBP1-31465-img0006.jpg)
![Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Western blot analysis of extracts of various cell lines, using Ndufs4 Rabbit mAb (NBP3-16679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-6T10F4-Western-Blot-NBP3-16679-img0006.jpg "Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Western blot analysis of extracts of various cell lines, using Ndufs4 Rabbit mAb (NBP3-16679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Immunofluorescence analysis of NIH-3T3 cells using Ndufs4 Rabbit mAb (NBP3-16679) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-6T10F4-Immunocytochemistry-Immunofluorescence-NBP3-16679-img0005.jpg)
![Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Western blot analysis of extracts of HepG2 cells, using Ndufs4 Rabbit mAb (NBP3-16679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-6T10F4-Western-Blot-NBP3-16679-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Immunofluorescence analysis of C6 cells using Ndufs4 Rabbit mAb (NBP3-16679) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-6T10F4-Immunocytochemistry-Immunofluorescence-NBP3-16679-img0004.jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Analysis of NDUFS4 in paraffin-embedded human colon carcinoma tissue using NDUFS4 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16679_rabbit-ndufs4-mab-6t10f4-immunohistochemistry-paraffin-272202491912..jpg)
![Immunohistochemistry-Paraffin: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Analysis of NDUFS4 in paraffin-embedded human liver tissue using NDUFS4 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16679_rabbit-ndufs4-mab-6t10f4-immunohistochemistry-paraffin-27220249233..jpg)
![Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody (M01), clone 1A1.Lane 1: NDUFS4 transfected lysate(20.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-1A1-Western-Blot-H00004724-M01-img0011.jpg "Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody (M01), clone 1A1.Lane 1: NDUFS4 transfected lysate(20.1 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-1A1-Immunohistochemistry-Paraffin-H00004724-M01-img0007.jpg)
![Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - Analysis of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi ( Cat # H00004724-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS4 monoclonal antibody (M01), clone 1A1 (Cat # H00004724-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-1A1-Western-Blot-H00004724-M01-img0008.jpg)
![Western Blot: Ndufs4 Antibody (1A1) [H00004724-M01] - NDUFS4 monoclonal antibody (M01), clone 1A1 Analysis of NDUFS4 expression in A-431.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-1A1-Western-Blot-H00004724-M01-img0010.jpg)
![Sandwich ELISA: Ndufs4 Antibody (1A1) [H00004724-M01] - Detection limit for recombinant GST tagged NDUFS4 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-1A1-Sandwich-ELISA-H00004724-M01-img0009.jpg)
![Immunohistochemistry: Ndufs4 Antibody (9H1) [NBP3-26116] - Image of Ndufs4 Antibody (9H1) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26116_rabbit-ndufs4-mab-9h1-27220248573312.jpg "Immunohistochemistry: Ndufs4 Antibody (9H1) [NBP3-26116] - Image of Ndufs4 Antibody (9H1) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Flow Cytometry: Ndufs4 Antibody (9H1) [NBP3-26116] - Overlay Peak curve showing MCF7 cells stained with Ndufs4 Antibody (9H1) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26116_rabbit-ndufs4-mab-9h1-282202414312761.jpg)
![Western Blot: Ndufs4 Overexpression Lysate (Adult Normal) [NBL1-13563] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ndufs4.](http://images.novusbio.com/fullsize/Ndufs4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13563-img0002.jpg "Western Blot: Ndufs4 Overexpression Lysate (Adult Normal) [NBL1-13563] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ndufs4.")
![Western Blot: Ndufs4 Antibody (3F11) [H00004724-M03] - Analysis of NDUFS4 expression in human kidney.](http://images.novusbio.com/fullsize/Ndufs4-Antibody-3F11-Western-Blot-H00004724-M03-img0001.jpg "Western Blot: Ndufs4 Antibody (3F11) [H00004724-M03] - Analysis of NDUFS4 expression in human kidney.")