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![Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of extracts from 9 different cell lines: (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Western-Blot-NBP1-47801-img0022.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of extracts from 9 different cell lines: (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRP75/HSPA9B/Mortalin.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunocytochemistry-Immunofluorescence-NBP1-47801-img0014.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0020.jpg)
![Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of Jurkat cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Flow-Cytometry-NBP1-47801-img0013.jpg)
![Western Blot: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRP75/HSPA9B/Mortalin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRP75/HSPA9B/Mortalin.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Western-Blot-NBP1-47801-img0021.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0015.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0016.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0017.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0018.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Staining of paraffin-embedded Human endometrium tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Immunohistochemistry-Paraffin-NBP1-47801-img0019.jpg)
![Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (9F8) [NBP1-47801] - Analysis of Hela cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9F8-Flow-Cytometry-NBP1-47801-img0023.jpg)
![Western Blot: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRP75/HSPA9B/Mortalin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-OTI9F8-Azide-and-BSA-Free-Western-Blot-NBP2-70963-img0002.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRP75/HSPA9B/Mortalin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti")
![Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRP75/HSPA9B/Mortalin.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-OTI9F8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70963-img0005.jpg)
![Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Staining of paraffin-embedded Human endometrium tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-OTI9F8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70963-img0010.jpg)
![Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Analysis of Hela cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-OTI9F8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70963-img0004.jpg)
![Western Blot: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Analysis of extracts from 9 different cell lines: (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-OTI9F8-Azide-and-BSA-Free-Western-Blot-NBP2-70963-img0001.jpg)
![Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-OTI9F8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70963-img0006.jpg)
![Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-OTI9F8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70963-img0007.jpg)
![Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-OTI9F8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70963-img0008.jpg)
![Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-OTI9F8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70963-img0009.jpg)
![Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Staining of paraffin-embedded Human pancreas tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-OTI9F8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70963-img0011.jpg)
![Flow Cytometry: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Analysis of Jurkat cells, using anti-Mortalin antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-OTI9F8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70963-img0003.jpg)
![Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Western-Blot-NBP1-31596-img0007.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Grp75 stained by Grp75 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31596-img0002.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Rat duodenum. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunohistochemistry-Paraffin-NBP1-31596-img0005.jpg)
![Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Whole cell extract (6 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP75 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Western-Blot-NBP1-31596-img0003.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Rat brain. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunohistochemistry-Paraffin-NBP1-31596-img0001.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Mouse duodenum. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunohistochemistry-Paraffin-NBP1-31596-img0004.jpg)
![Immunoprecipitation: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Immunoprecipitation of GRP75 protein from HepG2 whole cell extracts using 5 ug of GRP75 antibody. Western blot analysis was performed using GRP75 antibody diluted at 1:2500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunoprecipitation-NBP1-31596-img0008.jpg)
![Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-91020242041215.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.GRP75/HSPA9B/Mortalin stained by GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-91020242075965.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - GRP75/HSPA9B/Mortalin antibody detects GRP75/HSPA9B/Mortalin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.GRP75/HSPA9B/Mortalin stained by GRP75/HSPA9B/Mortalin antibody (NBP1-31596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31596_rabbit-polyclonal-grp75-hspa9b-mortalin-antibody-910202420115040.jpg)
![Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Analysis in human cell line CACO-2.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Western-Blot-NBP1-89729-img0019.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Analysis in human cell line CACO-2.")
![Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89729-img0014.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Staining of human cerebral cortex shows strong positivity in mitochondria in neurons.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunohistochemistry-Paraffin-NBP1-89729-img0020.jpg)
![Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Western-Blot-NBP1-89729-img0013.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Staining of human colon shows strong positivity in mitochondria in glandular cells.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunohistochemistry-Paraffin-NBP1-89729-img0016.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Staining of human liver shows strong positivity in mitochondria in hepatocytes.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunohistochemistry-Paraffin-NBP1-89729-img0017.jpg)
![Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-89729] - Staining of human kidney shows strong positivity in mitochondria in cells in tubules.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-Immunohistochemistry-Paraffin-NBP1-89729-img0018.jpg)
![Western Blot: Mortalin Antibody (30A5) [NBP1-97448] - Analysis of Grp75/Mortalin, mAb (30A5): Lane 1: MW marker; Lane 2: HeLa (heat shocked); Lane 3: L-929 (heat shocked); Lane 4: CHO -K1 (heat shocked); Lane 5: GPC-16 (heat shocked); Lane 6: PC-12 (heat shocked).](http://images.novusbio.com/fullsize/Mortalin-Antibody-30A5-Western-Blot-NBP1-97448-img0002.jpg "Western Blot: Mortalin Antibody (30A5) [NBP1-97448] - Analysis of Grp75/Mortalin, mAb (30A5): Lane 1: MW marker; Lane 2: HeLa (heat shocked); Lane 3: L-929 (heat shocked); Lane 4: CHO -K1 (heat shocked); Lane 5: GPC-16 (heat shocked); Lane 6: PC-12 (heat shocked).")
![Immunohistochemistry: Mortalin Antibody (30A5) [NBP1-97448] - Analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).](http://images.novusbio.com/fullsize/Mortalin-Antibody-30A5-Immunohistochemistry-NBP1-97448-img0001.jpg)
![Western Blot: GRP75/HSPA9B/Mortalin Antibody (6-A9-R) [NBP3-32407] - Western blot analysis of GRP75/HSPA9B/Mortalin on different lysates with Mouse anti-GRP75/HSPA9B/Mortalin antibody (NBP3-32407) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Rat kidney tissue lysate Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32407) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32407_mouse-grp75-hspa9b-mortalin-mab-6-a9-r-27820241631617.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody (6-A9-R) [NBP3-32407] - Western blot analysis of GRP75/HSPA9B/Mortalin on different lysates with Mouse anti-GRP75/HSPA9B/Mortalin antibody (NBP3-32407) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Rat kidney tissue lysate Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32407) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (6-A9-R) [NBP3-32407] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-GRP75/HSPA9B/Mortalin antibody (NBP3-32407) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32407) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32407_mouse-grp75-hspa9b-mortalin-mab-6-a9-r-298202414333663.jpg)
![Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (6-A9-R) [NBP3-32407] - Immunocytochemistry analysis of K-562 cells labeling GRP75/HSPA9B/Mortalin with Mouse anti-GRP75/HSPA9B/Mortalin antibody (NBP3-32407) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-GRP75/HSPA9B/Mortalin antibody (NBP3-32407) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32407_mouse-grp75-hspa9b-mortalin-mab-6-a9-r-29202410484821.jpg)
![SDS-Page: Recombinant Human GRP75/HSPA9B/Mortalin Protein [NBC1-18380]](http://images.novusbio.com/fullsize/Recombinant-Human-GRP75-HSPA9B-Mortalin-Protein-SDS-Page-NBC1-18380-img0003.jpg "SDS-Page: Recombinant Human GRP75/HSPA9B/Mortalin Protein [NBC1-18380]")
![ELISA: Mouse GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric) [NBP2-76446] - Samples were spiked with high concentrations of Mouse GRP75/HSPA9B/Mortalin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76446_mouse-grp75-hspa9b-mortalin-elisa-kit-colorimetric-13220241685351.jpg "ELISA: Mouse GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric) [NBP2-76446] - Samples were spiked with high concentrations of Mouse GRP75/HSPA9B/Mortalin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric) [NBP2-76446] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76446_mouse-grp75-hspa9b-mortalin-elisa-kit-colorimetric-432024932624.jpg)
![Immunoprecipitation:GRP75/HSPA9B/Mortalin AntibodyNBP3-29778] -Detection of human HSPA9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HSPA9 antibody NBP3-29778 (lot NBP3-29778-1) used for IP at 6 µg per reaction. HSPA9 was also immunoprecipitated by rabbit anti-HSPA9 antibody. for blotting immunoprecipitated HSPA9, NBP3-29778 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/nbp3-29778_rabbit-grp75-hspa9b-mortalin-pab-301220241536292.jpg "Immunoprecipitation:GRP75/HSPA9B/Mortalin AntibodyNBP3-29778] -Detection of human HSPA9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HSPA9 antibody NBP3-29778 (lot NBP3-29778-1) used for IP at 6 µg per reaction. HSPA9 was also immunoprecipitated by rabbit anti-HSPA9 antibody. for blotting immunoprecipitated HSPA9, NBP3-29778 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![Western Blot: GRP75/HSPA9B/Mortalin Antibody (9D6H0) [NBP3-15365] - Western blot analysis of extracts of various cell lines, using GRP75/HSPA9B/Mortalin Rabbit mAb (NBP3-15365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/GRP75-HSPA9B-Mortalin-Antibody-9D6H0-Western-Blot-NBP3-15365-img0001.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody (9D6H0) [NBP3-15365] - Western blot analysis of extracts of various cell lines, using GRP75/HSPA9B/Mortalin Rabbit mAb (NBP3-15365) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: GRP75/HSPA9B/Mortalin Antibody (SR2186) [NBP3-21787] - Western blot analysis using NBP3-21787 in (1) HeLa cell lysate; (2) Mouse liver lysate.](http://images.novusbio.com/fullsize/nbp3-21787_rabbit-grp75-hspa9b-mortalin-mab-sr2186-57202317161833.jpg "Western Blot: GRP75/HSPA9B/Mortalin Antibody (SR2186) [NBP3-21787] - Western blot analysis using NBP3-21787 in (1) HeLa cell lysate; (2) Mouse liver lysate.")
![ELISA: Rat GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric) [NBP2-76447] - Samples were spiked with high concentrations of Rat GRP75/HSPA9B/Mortalin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76447_rat-grp75-hspa9b-mortalin-elisa-kit-colorimetric-132202416254067.jpg "ELISA: Rat GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric) [NBP2-76447] - Samples were spiked with high concentrations of Rat GRP75/HSPA9B/Mortalin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric) [NBP2-76447] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76447_rat-grp75-hspa9b-mortalin-elisa-kit-colorimetric-432024859755.jpg)