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GRP75/HSPA9B/Mortalin Antibody (30A5)

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Western Blot: Mortalin Antibody (30A5) [NBP1-97448] - Analysis of Grp75/Mortalin, mAb (30A5): Lane 1: MW marker; Lane 2: HeLa (heat shocked); Lane 3: L-929 (heat shocked); Lane 4: CHO -K1 (heat shocked); Lane 5: GPC-16 (heat shocked); Lane 6: PC-12 (heat shocked).
Western Blot: Mortalin Antibody (30A5) [NBP1-97448] - Analysis of Grp75/Mortalin, mAb (30A5): Lane 1: MW marker; Lane 2: HeLa (heat shocked); Lane 3: L-929 (heat shocked); Lane 4: CHO -K1 (heat shocked); Lane 5: GPC-16 ...read more
Immunohistochemistry: Mortalin Antibody (30A5) [NBP1-97448] - Analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Gp, Ha, Pm, Rb, Sh, XpSpecies Glossary
Applications WB, IHC
Clone
30A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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GRP75/HSPA9B/Mortalin Antibody (30A5) Summary

Immunogen
Native human Grp75 (Mortalin).
Marker
Immortalization Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPA9
Purity
Ammonium sulfate precipitation
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
Application Notes
This antibody is useful in Immunohistochemistry and Western Blot (Detects a band of ~75kDa).
Publications
Read Publication using
NBP1-97448 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for GRP75/HSPA9B/Mortalin Antibody (30A5)

  • 75 kDa glucose-regulated protein
  • CSA
  • GRP75
  • GRP-75
  • GRP75mthsp75
  • Heat shock 70 kDa protein 9
  • heat shock 70kD protein 9B
  • heat shock 70kDa protein 9 (mortalin)
  • heat shock 70kDa protein 9B (mortalin-2)
  • HSPA9
  • HSPA9B
  • HSPA9BMGC4500
  • Mortalin
  • mortalin, perinuclear
  • Mortalin-2
  • MOT
  • MOT2
  • mot-2
  • MTHSP75
  • p66-mortalin
  • PBP74
  • PBP74CSA
  • Peptide-binding protein 74
  • stress-70 protein, mitochondrial

Background

Grp75 (Glucose regulated 75 kDa protein) is a member of the Hsp70 family. It is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the re-folding of proteins translocated into this organelle. Like its E. coli homolog DnaK, Grp75 possesses a cation-dependent ATPase activity thought to be central to its function as a chaperone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GRP75/HSPA9B/Mortalin Antibody (NBP1-97448)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HSPA9