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![Immunocytochemistry/Immunofluorescence: Ly-6E Antibody [NBP1-52176] - Immunofluorescence analysis of paraformaldehyde fixed L929 cells (Mouse fibroblast cell line), permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Ly-6E-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-52176-img0003.jpg "Immunocytochemistry/Immunofluorescence: Ly-6E Antibody [NBP1-52176] - Immunofluorescence analysis of paraformaldehyde fixed L929 cells (Mouse fibroblast cell line), permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Flow Cytometry: Ly-6E Antibody [NBP1-52176] - Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Ly-6E-Antibody-Flow-Cytometry-NBP1-52176-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Ly-6E Antibody [NBP1-52176] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Ly-6E-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-52176-img0002.jpg)
![Western Blot: Ly-6E Antibody [NBP3-03570] - Analysis of extracts of various cell lines, using Ly-6E antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/Ly-6E-Antibody-Western-Blot-NBP3-03570-img0002.jpg "Western Blot: Ly-6E Antibody [NBP3-03570] - Analysis of extracts of various cell lines, using Ly-6E antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/Immunofluorescence: Ly-6E Antibody [NBP3-03570] - Analysis of L929 cells using LY6E Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. Ly-6E antibody](http://images.novusbio.com/fullsize/Ly-6E-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03570-img0001.jpg)
![Immunohistochemistry-Paraffin: Ly-6E Antibody [NBP3-03570] - Left panel shows negative control antibody on mouse kidney; right panel shows Ly-6E antibody at 1:100 dilution on mouse kidney. Brown = DAB reaction. Mouse kidneys were deparaffinized through xylenes and alcohols, boiled in citrate buffer for 25 minutes, then PBS-Tween at room temp for 5 minutes, followed by Scytek CRF anti-polyvalent polymer DAB kit protocol. Slides were counterstained for 30 seconds in hematoxylin and coverslipped. Image from verified customer review.](http://images.novusbio.com/fullsize/Ly-6E-Antibody-Immunohistochemistry-Paraffin-NBP3-03570-img0003.jpg)
![Immunohistochemistry-Paraffin: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] - LY6E antibody [HL1933] detects LY6E protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. LY6E stained by LY6E antibody [HL1933] (NBP3-25553) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25553_rabbit-ly-6e-mab-hl1933-azide-and-bsa-free-212024940894.jpg "Immunohistochemistry-Paraffin: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] - LY6E antibody [HL1933] detects LY6E protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human placenta. LY6E stained by LY6E antibody [HL1933] (NBP3-25553) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (NBP3-25553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25553_rabbit-ly-6e-mab-hl1933-azide-and-bsa-free-212024941423.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Ly-6E Antibody (HL1933) - Azide and BSA Free [NBP3-25553] - Non-transfected (-) and transfected (+) A549 whole cell extract (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with LY6E antibody [HL1933] (NBP3-25553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25553_rabbit-ly-6e-mab-hl1933-azide-and-bsa-free-2120249403880.jpg)
![Western Blot: Ly-6E Overexpression Lysate (Adult Normal) [NBL1-12748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ly-6E.](http://images.novusbio.com/fullsize/Ly-6E-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12748-img0002.jpg "Western Blot: Ly-6E Overexpression Lysate (Adult Normal) [NBL1-12748] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ly-6E.")
![Western Blot: Ly-6E Overexpression Lysate (Adult Normal) [NBP2-11388] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ly-6E.](http://images.novusbio.com/fullsize/Ly-6E-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11388-img0001.jpg "Western Blot: Ly-6E Overexpression Lysate (Adult Normal) [NBP2-11388] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ly-6E.")
![Western Blot: Ly-6E Antibody [H00004061-D01P] - Analysis of LY6E expression in transfected 293T cell line by LY6E polyclonal antibody.Lane 1: LY6E transfected lysate(13.50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Ly-6E-Antibody-Western-Blot-H00004061-D01P-img0002.jpg "Western Blot: Ly-6E Antibody [H00004061-D01P] - Analysis of LY6E expression in transfected 293T cell line by LY6E polyclonal antibody.Lane 1: LY6E transfected lysate(13.50 KDa).Lane 2: Non-transfected lysate.")