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![Immunohistochemistry-Paraffin: LRPAP Antibody [NBP1-89334] - Staining in human placenta and skeletal muscle tissues.. Corresponding LRPAP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89334-img0025.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LRPAP Antibody [NBP1-89334] - Staining in human placenta and skeletal muscle tissues.. Corresponding LRPAP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: LRPAP Antibody [NBP1-89334] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/LRPAP-Antibody-Western-Blot-NBP1-89334-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: LRPAP Antibody [NBP1-89334] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89334-img0017.jpg)
![Immunohistochemistry-Paraffin: LRPAP Antibody [NBP1-89334] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89334-img0030.jpg)
![Western Blot: LRPAP Antibody [NBP1-89334] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/LRPAP-Antibody-Western-Blot-NBP1-89334-img0005.jpg)
![Western Blot: LRPAP Antibody [NBP1-89334] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).](http://images.novusbio.com/fullsize/LRPAP-Antibody-Western-Blot-NBP1-89334-img0004.jpg)
![Western Blot: LRPAP Antibody [NBP1-89334] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/LRPAP-Antibody-Western-Blot-NBP1-89334-img0009.jpg)
![Immunohistochemistry-Paraffin: LRPAP Antibody [NBP1-89334] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89334-img0018.jpg)
![Immunohistochemistry: LRPAP Antibody [NBP1-89334] - Immunofluorescence staining of mouse hypothalamus shows positivity in paraventricular and periventricular nuclei.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89334-img0019.jpg)
![Immunohistochemistry-Paraffin: LRPAP Antibody [NBP1-89334] - Staining of human hippocampus shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89334-img0020.jpg)
![Immunohistochemistry: LRPAP Antibody [NBP1-89334] - Immunofluorescence staining of mouse brain shows positivity in neurons in the olfactory bulb.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89334-img0021.jpg)
![Immunohistochemistry-Paraffin: LRPAP Antibody [NBP1-89334] - Staining of human skeletal muscle shows weak to no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89334-img0022.jpg)
![Immunohistochemistry-Paraffin: LRPAP Antibody [NBP1-89334] - Staining of human placenta shows strong positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP1-89334-img0023.jpg)
![Immunohistochemistry: LRPAP Antibody [NBP1-89334] - Staining of mouse piriform cortex shows positivity in neurons.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-NBP1-89334-img0028.jpg)
![Immunohistochemistry: LRPAP Antibody [NBP1-89334] - Staining of mouse somatosensory cortex shows positivity in neurons.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-NBP1-89334-img0029.jpg)
![Western Blot: LRPAP Antibody [NBP2-15088] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LRPAP antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Western-Blot-NBP2-15088-img0008.jpg "Western Blot: LRPAP Antibody [NBP2-15088] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LRPAP antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: LRPAP Antibody [NBP2-15088] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LRPAP protein stained by LRPAP antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15088-img0004.jpg)
![Immunohistochemistry-Paraffin: LRPAP Antibody [NBP2-15088] - Sample: Paraffin-embedded rat brain. LRPAP antibody diluted at 1:500.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP2-15088-img0005.jpg)
![Western Blot: LRPAP Antibody [NBP2-15088] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LRPAP antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Western-Blot-NBP2-15088-img0006.jpg)
![Western Blot: LRPAP Antibody [NBP2-15088] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LRPAP antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Western-Blot-NBP2-15088-img0007.jpg)
![Western Blot: LRPAP Antibody (009) [NBP2-90513] - Anti-LRPAP rabbit monoclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa](http://images.novusbio.com/fullsize/LRPAP-Antibody-009-Western-Blot-NBP2-90513-img0003.jpg "Western Blot: LRPAP Antibody (009) [NBP2-90513] - Anti-LRPAP rabbit monoclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa")
![Immunocytochemistry/Immunofluorescence: LRPAP Antibody (009) [NBP2-90513] - Immunofluorescence staining of mouse LRPAP in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse LRPAP monoclonal antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Endoplasmic reticulum.](http://images.novusbio.com/fullsize/LRPAP-Antibody-009-Immunocytochemistry-Immunofluorescence-NBP2-90513-img0002.jpg)
![Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513] - Immunochemical staining of mouse LRPAP in mouse brain with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. The image showing staining of nerve cells.](http://images.novusbio.com/fullsize/LRPAP-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90513-img0005.jpg)
![Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513] - Immunochemical staining of mouse LRPAP in mouse brain with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. The image showing staining of nephric tubule.](http://images.novusbio.com/fullsize/LRPAP-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90513-img0004.jpg)
![Immunoprecipitation: LRPAP Antibody (009) [NBP2-90513] - Mouse LRPAP was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate0.5 ul anti-Mouse LRPAP rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse LRPAP rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa](http://images.novusbio.com/fullsize/LRPAP-Antibody-009-Immunoprecipitation-NBP2-90513-img0006.jpg)
![Western Blot: LRPAP Antibody [NBP2-99704] - Lane A: U87-MG Whole Cell LysateLane B: PC-3 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:41 kDa](http://images.novusbio.com/fullsize/LRPAP-Antibody-Western-Blot-NBP2-99704-img0007.jpg "Western Blot: LRPAP Antibody [NBP2-99704] - Lane A: U87-MG Whole Cell LysateLane B: PC-3 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:41 kDa")
![Immunohistochemistry-Paraffin: LRPAP Antibody [NBP2-99704] - Staining of human LRPAP in human liver with rabbit polyclonal antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP2-99704-img0005.jpg)
![Immunohistochemistry-Paraffin: LRPAP Antibody [NBP2-99704] - Staining of human LRPAP in human brain with rabbit polyclonal antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP2-99704-img0003.jpg)
![Immunohistochemistry-Paraffin: LRPAP Antibody [NBP2-99704] - Staining of human LRPAP in human kidney with rabbit polyclonal antibody at 1:5000 dilution.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-Paraffin-NBP2-99704-img0004.jpg)
![Immunoprecipitation: LRPAP Antibody [NBP2-99704] - Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate0.5 uL anti-LRPAP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LRPAP1 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size: 41 kDa](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunoprecipitation-NBP2-99704-img0006.jpg)
![Western Blot: LRPAP Antibody (4) [NBP2-89796] - Lane A: U87-MG Whole Cell Lysate Lane B: PC-3 Whole Cell LysateLane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDa Observed band size:41 kDa](http://images.novusbio.com/fullsize/LRPAP-Antibody-4-Western-Blot-NBP2-89796-img0002.jpg "Western Blot: LRPAP Antibody (4) [NBP2-89796] - Lane A: U87-MG Whole Cell Lysate Lane B: PC-3 Whole Cell LysateLane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:41 kDa Observed band size:41 kDa")
![Immunocytochemistry/Immunofluorescence: LRPAP Antibody (4) [NBP2-89796] - Staining of Human LRPAP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human LRPAP1 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/LRPAP-Antibody-4-Immunocytochemistry-Immunofluorescence-NBP2-89796-img0001.jpg)
![Immunoprecipitation: LRPAP Antibody (4) [NBP2-89796] - Lane A:0.5 mg 293T Whole Cell Lysate 2 uL anti-LRPAP1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LRPAP1 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 41 kDa Observed band size: 41 kDa](http://images.novusbio.com/fullsize/LRPAP-Antibody-4-Immunoprecipitation-NBP2-89796-img0003.jpg)
![Western Blot: LRPAP Antibody (7F1) - Chimeric [NBP3-12047] - Staining in HeLa(A) (0.1ug/ml), A549 (B) (0.01ug/ml), HepG2 (C) (0.01ug/ml), HEK293 (D) (0.03ug/ml), MCF7 (E) (0.03ug/ml), Caco-2 (F) (0.001ug/ml) and U251 (G) (0.001ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAP (NBP3-12047) at 0.5 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/LRPAP-Antibody-7F1-Chimeric-Western-Blot-NBP3-12047-img0001.jpg "Western Blot: LRPAP Antibody (7F1) - Chimeric [NBP3-12047] - Staining in HeLa(A) (0.1ug/ml), A549 (B) (0.01ug/ml), HepG2 (C) (0.01ug/ml), HEK293 (D) (0.03ug/ml), MCF7 (E) (0.03ug/ml), Caco-2 (F) (0.001ug/ml) and U251 (G) (0.001ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAP (NBP3-12047) at 0.5 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: LRPAP Antibody (7F1) - Chimeric [NBP3-12047] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 7F1 (NBP3-12047) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12047, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/LRPAP-Antibody-7F1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12047-img0002.jpg)
![Western Blot: LRPAP Antibody [NBP1-69362] - This Anti-LRPAP1 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 2.5ug/ml.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Western-Blot-NBP1-69362-img0001.jpg "Western Blot: LRPAP Antibody [NBP1-69362] - This Anti-LRPAP1 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 2.5ug/ml.")
![Immunohistochemistry: LRPAP Antibody [NBP1-69362] - Human kidney.](http://images.novusbio.com/fullsize/LRPAP-Antibody-Immunohistochemistry-NBP1-69362-img0002.jpg)