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![Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP2-38956] - Staining in human testis and liver tissues using NBP2-38956 antibody. Corresponding IGF2BP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Immunohistochemistry-Paraffin-NBP2-38956-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP2-38956] - Staining in human testis and liver tissues using NBP2-38956 antibody. Corresponding IGF2BP1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP2-38956] - Staining of human endometrium, ovary, pancreas and testis using Anti-IGF2BP1 antibody NBP2-38956 (A) shows similar protein distribution across tissues to independent antibody NBP1-83108 (B).](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Immunohistochemistry-Paraffin-NBP2-38956-img0013.jpg)
![Western Blot: IGF2BP1 Antibody [NBP2-38956] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line CACO-2](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Western-Blot-NBP2-38956-img0007.jpg)
![Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP2-38956] - Staining of human ovary shows moderate cytoplasmic positivity in follicle cells.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Immunohistochemistry-Paraffin-NBP2-38956-img0018.jpg)
![Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP2-38956] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Immunohistochemistry-Paraffin-NBP2-38956-img0012.jpg)
![Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP2-38956] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Immunohistochemistry-Paraffin-NBP2-38956-img0014.jpg)
![Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP2-38956] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Immunohistochemistry-Paraffin-NBP2-38956-img0015.jpg)
![Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP2-38956] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Immunohistochemistry-Paraffin-NBP2-38956-img0016.jpg)
![Simple Western: IGF2BP1 Antibody [NBP2-38956] - Simple Western lane view shows a specific band for IGF2BP1 in 0.2 mg/ml of HT-29 (left) and HCT 116 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation systems.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Simple-Western-NBP2-38956-img0002.jpg)
![Simple Western: IGF2BP1 Antibody [NBP2-38956] - Electropherogram image of the corresponding Simple Western lane view. IGF2BP1 antibody was used at 1:10 dilution on HT-29 and HCT 116 lysate(s) respectively.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Simple-Western-NBP2-38956-img0003.jpg)
![Immunohistochemistry: IGF2BP1 Antibody [NBP3-35508] - Immunohistochemistry analysis of paraffin-embedded Human stomach using IGF2BP1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35508_rabbit-igf2bp1-pab-1012202411364614.jpg "Immunohistochemistry: IGF2BP1 Antibody [NBP3-35508] - Immunohistochemistry analysis of paraffin-embedded Human stomach using IGF2BP1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: IGF2BP1 Antibody [NBP3-35508] - Immunofluorescence analysis of U2OS cells using IGF2BP1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35508_rabbit-igf2bp1-pab-1012202411392930.jpg)
![Western Blot: IGF2BP1 Antibody [NBP3-35508] - Western blot analysis of various lysates using IGF2BP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35508_rabbit-igf2bp1-pab-1012202411334056.jpg)
![Western Blot: IGF2BP1 Antibody [NBP1-57495] - HepG2 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Western-Blot-NBP1-57495-img0002.jpg "Western Blot: IGF2BP1 Antibody [NBP1-57495] - HepG2 cell lysate, concentration 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP1-57495] - Human lung adenocarcinoma](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-57495-img0006.jpg)
![Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP1-57495] - Human lung adenocarcinoma.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-57495-img0005.jpg)
![Western Blot: IGF2BP1 Antibody [NBP1-52044] - (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Western-Blot-NBP1-52044-img0002.jpg "Western Blot: IGF2BP1 Antibody [NBP1-52044] - (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: IGF2BP1 Antibody [NBP1-52044] - 3.75ug/ml staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-52044-img0003.jpg)
![Immunoprecipitation: IGF2BP1 Antibody [NBP3-35366] - Immunoprecipitation analysis of 200 ug extracts of K562 cells using IGF2BP1 antibody. Western blot was performed from the immunoprecipitate using IGF2BP1/IMP1 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35366_rabbit-igf2bp1-pab-1012202411545585.jpg "Immunoprecipitation: IGF2BP1 Antibody [NBP3-35366] - Immunoprecipitation analysis of 200 ug extracts of K562 cells using IGF2BP1 antibody. Western blot was performed from the immunoprecipitate using IGF2BP1/IMP1 antibody at a dilution of 1:1000.")
![Western Blot: IGF2BP1 Antibody [NBP3-35366] - Western blot analysis of various lysates using IGF2BP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/nbp3-35366_rabbit-igf2bp1-pab-10122024121022.jpg)
![Western Blot: IGF2BP1 Antibody [NBP2-36473] - WB Suggested Anti-Igf2bp1 Antibody. Titration: 1.0 ug/ml. Positive Control: Mouse Thymus](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Western-Blot-NBP2-36473-img0002.jpg "Western Blot: IGF2BP1 Antibody [NBP2-36473] - WB Suggested Anti-Igf2bp1 Antibody. Titration: 1.0 ug/ml. Positive Control: Mouse Thymus")
![Western Blot: IGF2BP1 Antibody [NBP2-36473] - Titration: 1.0 ug/ml Positive Control: Mouse Thymus](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Western-Blot-NBP2-36473-img0001.jpg)
![Western Blot: IGF2BP1 Antibody [NBP2-36473] - Host: Rat. Target Name: IGF2BP1. Sample Tissue: Rat Skeletal Muscle. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Western-Blot-NBP2-36473-img0003.jpg)
![Immunohistochemistry-Paraffin: IGF2BP1 Antibody (SR2428) [NBP3-27730] - Immunohistochemistry analysis of paraffin-embedded mouse colon. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.](http://images.novusbio.com/fullsize/nbp3-27730_rabbit-igf2bp1-mab-sr2428-75202410133426.jpg "Immunohistochemistry-Paraffin: IGF2BP1 Antibody (SR2428) [NBP3-27730] - Immunohistochemistry analysis of paraffin-embedded mouse colon. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.")
![Western Blot: IGF2BP1 Antibody (SR2428) [NBP3-27730] - Western blot analysis of IGF2BP1 in K562, 3T3 lysates](http://images.novusbio.com/fullsize/nbp3-27730_rabbit-igf2bp1-mab-sr2428-752024120321.jpg)
![Immunoprecipitation: IGF2BP1 Antibody [NBP1-79024] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IMP1 antibody used for IP at 6 mcg/mg lysate. IMP1 was not successfully immunoprecipitated by rabbit anti-IMP1 antibody NBP1-79023, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Immunoprecipitation-NBP1-79024-img0002.jpg "Immunoprecipitation: IGF2BP1 Antibody [NBP1-79024] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IMP1 antibody used for IP at 6 mcg/mg lysate. IMP1 was not successfully immunoprecipitated by rabbit anti-IMP1 antibody NBP1-79023, which recognizes an upstream epitope.")
![Western Blot: IGF2BP1 Antibody [NBP1-79023] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-IMP1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IMP1 was successfully immunoprecipitated by rabbit anti-IMP1 antibody NBP1-79024 which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/IGF2BP1-Antibody-Western-Blot-NBP1-79023-img0002.jpg "Western Blot: IGF2BP1 Antibody [NBP1-79023] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-IMP1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. IMP1 was successfully immunoprecipitated by rabbit anti-IMP1 antibody NBP1-79024 which recognizes a downstream epitope.")
![Western Blot: IGF2BP1 Overexpression Lysate (Adult Normal) [NBL1-11870] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IGF2BP1.](http://images.novusbio.com/fullsize/IGF2BP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11870-img0002.jpg "Western Blot: IGF2BP1 Overexpression Lysate (Adult Normal) [NBL1-11870] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IGF2BP1.")