Hu,
Mu,
Rt,
Po,
Bv,
Ca,
Ch,
Gp,
Ha,
Md,
Pm,
Rb,
Sh,
XpSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Gp, Ha, Md, Pm, Rb, Sh, Xp
Applications: WB, IHC, IP
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Immunohistochemistry-Paraffin: HOP Antibody [NBP1-92003] - Staining in human skin and liver tissues . Corresponding HOPX RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HOP-Antibody-Immunohistochemistry-Paraffin-NBP1-92003-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HOP Antibody [NBP1-92003] - Staining in human skin and liver tissues . Corresponding HOPX RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HOP Antibody [NBP1-92003] - Staining of human cervix, uterine shows moderate positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/HOP-Antibody-Immunohistochemistry-Paraffin-NBP1-92003-img0006.jpg)
![Immunohistochemistry-Paraffin: HOP Antibody [NBP1-92003] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/HOP-Antibody-Immunohistochemistry-Paraffin-NBP1-92003-img0007.jpg)
![Immunohistochemistry-Paraffin: HOP Antibody [NBP1-92003] - Staining of human skin shows moderate positivity in stratum granulosum.](http://images.novusbio.com/fullsize/HOP-Antibody-Immunohistochemistry-Paraffin-NBP1-92003-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: HOP Antibody [NBP3-35571] - Immunofluorescence analysis of paraffin-embedded Human skin using HOP Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-35571_rabbit-hop-pab-912202414201364.jpg "Immunocytochemistry/ Immunofluorescence: HOP Antibody [NBP3-35571] - Immunofluorescence analysis of paraffin-embedded Human skin using HOP Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.")
![Western Blot: HOP Antibody [NBP3-35571] - Western blot analysis of lysates from Rat heart, using HOP Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35571_rabbit-hop-pab-912202415161816.jpg)
![Western Blot: HOP Antibody [NBP2-82093] - Western blot analysis of HOP in human brain tissue lysate with HOP antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/HOP-Antibody-Western-Blot-NBP2-82093-img0001.jpg "Western Blot: HOP Antibody [NBP2-82093] - Western blot analysis of HOP in human brain tissue lysate with HOP antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: HOP Antibody - BSA Free [NBP2-82093] - Immunofluorescence of HOP in human heart tissue with HOP antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82093_rabbit-polyclonal-hop-antibody-2711202420414571.jpg)
![ELISA: HOP Antibody (3D6) [H00084525-M01] - Detection limit for recombinant GST tagged HOP is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HOP-Antibody-3D6-ELISA-H00084525-M01-img0001.jpg "ELISA: HOP Antibody (3D6) [H00084525-M01] - Detection limit for recombinant GST tagged HOP is approximately 3ng/ml as a capture antibody.")
![ELISA: HOP Antibody (3D6) [H00084525-M01] - Detection limit for recombinant GST tagged HOP is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HOP-Antibody-3D6-ELISA-H00084525-M01-img0003.jpg)
![Western Blot: HOP Antibody (S06-4F8) [NBP3-19962] - Western blot detection of HOP in Hela cell lysates using NBP3-19962 (1:1000 diluted). Predicted band size: 8kDa. Observed band size: 12kDa.](http://images.novusbio.com/fullsize/HOP-Antibody-S06-4F8-Western-Blot-NBP3-19962-img0001.jpg "Western Blot: HOP Antibody (S06-4F8) [NBP3-19962] - Western blot detection of HOP in Hela cell lysates using NBP3-19962 (1:1000 diluted). Predicted band size: 8kDa. Observed band size: 12kDa.")
![Western Blot: HOP Overexpression Lysate (Adult Normal) [NBP2-05584] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HOP.](http://images.novusbio.com/fullsize/HOP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05584-img0001.jpg "Western Blot: HOP Overexpression Lysate (Adult Normal) [NBP2-05584] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HOP.")
![Western Blot: HOP Overexpression Lysate (Adult Normal) [NBL1-11661] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HOP.](http://images.novusbio.com/fullsize/HOP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11661-img0002.jpg "Western Blot: HOP Overexpression Lysate (Adult Normal) [NBL1-11661] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HOP.")
![Western Blot: HOP Overexpression Lysate (Adult Normal) [NBP2-11343] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HOP.](http://images.novusbio.com/fullsize/HOP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11343-img0001.jpg "Western Blot: HOP Overexpression Lysate (Adult Normal) [NBP2-11343] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HOP.")
![Western Blot: HOP Overexpression Lysate (Adult Normal) [NBP2-05585] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HOP.](http://images.novusbio.com/fullsize/HOP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-05585-img0001.jpg "Western Blot: HOP Overexpression Lysate (Adult Normal) [NBP2-05585] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HOP.")
![SDS-Page: Recombinant Human HOP Protein [NBP1-98893] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-HOP-Protein-SDS-Page-NBP1-98893-img0001.jpg "SDS-Page: Recombinant Human HOP Protein [NBP1-98893] - 15% SDS-PAGE (3ug)")
![Immunohistochemistry-Paraffin: HOP Antibody [NBP2-97809] - Immunochemical staining of human HOP in human brain with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HOP-Antibody-Immunohistochemistry-Paraffin-NBP2-97809-img0001.jpg "Immunohistochemistry-Paraffin: HOP Antibody [NBP2-97809] - Immunochemical staining of human HOP in human brain with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.")