Recombinant Human HOP His Protein Summary
Description |
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-73 of Human HOP Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSAETA SGPTEDQVEI LEYNFNKVDK HPDSTTLCLI AAEAGLSEEE TQKWFKQRLA KWRRSEGLPS ECRSVTD |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
HOPX |
Purity |
>90%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
10.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>90%, by SDS-PAGE |
Alternate Names for Recombinant Human HOP His Protein
Background
HOPX, also known as homeodomain-only protein, acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. This protein prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy. Recombinant human HOPX protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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