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![Western Blot: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.Positive control:Lane 1: SiHa Lane 2: Mouse colonLane 3: Rat liver](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Western-Blot-NBP2-75513-img0008.jpg "Western Blot: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.Positive control:Lane 1: SiHa Lane 2: Mouse colonLane 3: Rat liver")
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Staining Glutathione Synthetase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunocytochemistry-Immunofluorescence-NBP2-75513-img0002.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded rat epididymis tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0007.jpg)
![Flow Cytometry: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of HepG2 cells with Glutathione Synthetase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Flow-Cytometry-NBP2-75513-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human colon cancer tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human kidney tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-JB95-33-Immunohistochemistry-Paraffin-NBP2-75513-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining in human epididymis and pancreas tissues using anti-GSS antibody. Corresponding GSS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining in human epididymis and pancreas tissues using anti-GSS antibody. Corresponding GSS RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cerebral cortex, colon, epididymis and testis using Anti-GSS antibody NBP2-57177 (A) shows similar protein distribution across tissues to independent antibody NBP2-30465 (B).](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Glutathione Synthetase Antibody [NBP2-57177] - Analysis using Anti-GSS antibody NBP2-57177 (A) shows similar pattern to independent antibody NBP2-30465 (B).](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Western-Blot-NBP2-57177-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57177-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human testis.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human colon.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0010.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0011.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human epididymis using Anti-GSS antibody NBP2-57177.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0013.jpg)
![Western Blot: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutathione Synthetase.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Western-Blot-NBP2-03351-img0002.jpg "Western Blot: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Glutathione Synthetase.")
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] Staining of paraffin-embedded Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03351-img0005.jpg)
![Flow Cytometry: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Glutathione Synthetase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Flow-Cytometry-NBP2-03351-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03351-img0003.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (1B8) [NBP2-03351] - Staining of paraffin-embedded Human Kidney tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-1B8-Immunohistochemistry-Paraffin-NBP2-03351-img0004.jpg)
![Western Blot: Glutathione Synthetase Antibody [NBP3-03702] - Analysis of extracts of various cell lines, using Glutathione Synthetase (GSS) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.analysis of extracts of various cell lines, using Glutathione Synthetase (GSS) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Western-Blot-NBP3-03702-img0004.jpg "Western Blot: Glutathione Synthetase Antibody [NBP3-03702] - Analysis of extracts of various cell lines, using Glutathione Synthetase (GSS) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.analysis of extracts of various cell lines, using Glutathione Synthetase (GSS) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody [NBP3-03702] - Analysis of PC-12 cells using Glutathione Synthetase (GSS) antibody at dilution of 1:150. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03702-img0005.jpg)
![Western Blot: Glutathione Synthetase Antibody [NBP3-03702] - WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Western-Blot-NBP3-03702-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Glutathione Synthetase Antibody [NBP3-03702] - Analysis of extracts from normal (control) and GSS knockout (KO) 293T cells, using Glutathione Synthetase antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Knockout-Validated-NBP3-03702-img0001.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Analysis in human epididymis and skeletal muscle tissues using NBP2-30465antibody. Corresponding GSS RNA-seq data are presented for the same tissues](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Analysis in human epididymis and skeletal muscle tissues using NBP2-30465antibody. Corresponding GSS RNA-seq data are presented for the same tissues")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human fallopian tube, gastrointestinal, prostate and skeletal muscle using Anti-GSS antibody NBP2-30465 (A) shows similar protein distribution across tissues to independent antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0020.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Glutathione Synthetase Antibody [NBP2-30465] - Analysis using Anti-GSS antibody NBP2-30465 (A) shows similar pattern to independent antibody NBP2-57177 (B).](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Western-Blot-NBP2-30465-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30465-img0009.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0019.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0015.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human fallopian tube shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0016.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-30465] - Staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-30465-img0017.jpg)
![Western Blot: Glutathione Synthetase Antibody (2S9W7) [NBP3-15402] - Western blot analysis of extracts of various cell lines, using Glutathione Synthetase (GSS) Rabbit mAb (NBP3-15402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-2S9W7-Western-Blot-NBP3-15402-img0002.jpg "Western Blot: Glutathione Synthetase Antibody (2S9W7) [NBP3-15402] - Western blot analysis of extracts of various cell lines, using Glutathione Synthetase (GSS) Rabbit mAb (NBP3-15402) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody (2S9W7) [NBP3-15402] - Immunofluorescence analysis of NIH-3T3 cells using Glutathione Synthetase (GSS) Rabbit mAb (NBP3-15402) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-2S9W7-Immunocytochemistry-Immunofluorescence-NBP3-15402-img0001.jpg)
![Western Blot: Glutathione Synthetase Antibody (S07-2B0) [NBP3-19867] - Western blot detection of Glutathione Synthetase in Hela, A549 cell lysates using NBP3-19867 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-S07-2B0-Western-Blot-NBP3-19867-img0002.jpg "Western Blot: Glutathione Synthetase Antibody (S07-2B0) [NBP3-19867] - Western blot detection of Glutathione Synthetase in Hela, A549 cell lysates using NBP3-19867 (1:1000 diluted). Predicted band size: 52kDa. Observed band size: 52kDa.")
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (S07-2B0) [NBP3-19867] - Immunohistochemistry of Glutathione Synthetase in paraffin-embedded Human Cholangiocarcinoma using NBP3-19867 at dilution 1/50](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-S07-2B0-Immunohistochemistry-Paraffin-NBP3-19867-img0001.jpg)
![Western Blot: Glutathione Synthetase Antibody (4B6) [H00002937-M03] - Analysis of GSS expression in NSO.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-4B6-Western-Blot-H00002937-M03-img0002.jpg "Western Blot: Glutathione Synthetase Antibody (4B6) [H00002937-M03] - Analysis of GSS expression in NSO.")
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (4B6) [H00002937-M03] - Analysis of monoclonal antibody to GSS on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 1 ~ 10 ug/ml](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-4B6-Immunohistochemistry-Paraffin-H00002937-M03-img0001.jpg)
![Western Blot: Glutathione Synthetase Antibody (12F2) [NBP2-59455] - Lane 1: MDA-MB231 cell lysate, Lane 2: WiDr cell lysate, Lane 3: SW480 cell lysate, Lane 4.: A427 cell lysate, Lane 5: MCF7 cell lysate Lane 6: 293T cell lysate, Lane 7: HeLa cell lysate, Lane 8: HT1080 cell lysate Lane 9: U87MG cell lysate, Lane 10: A549 cell lysate](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-12F2-Western-Blot-NBP2-59455-img0001.jpg "Western Blot: Glutathione Synthetase Antibody (12F2) [NBP2-59455] - Lane 1: MDA-MB231 cell lysate, Lane 2: WiDr cell lysate, Lane 3: SW480 cell lysate, Lane 4.: A427 cell lysate, Lane 5: MCF7 cell lysate Lane 6: 293T cell lysate, Lane 7: HeLa cell lysate, Lane 8: HT1080 cell lysate Lane 9: U87MG cell lysate, Lane 10: A549 cell lysate")
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody (12F2) [NBP2-59455] - Analysis of GSS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of GSS in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-12F2-Immunocytochemistry-Immunofluorescence-NBP2-59455-img0002.jpg)
![Western Blot: Glutathione Synthetase Overexpression Lysate (Adult Normal) [NBL1-11365] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glutathione Synthetase.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11365-img0002.jpg "Western Blot: Glutathione Synthetase Overexpression Lysate (Adult Normal) [NBL1-11365] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glutathione Synthetase.")
![Western Blot: Glutathione Synthetase Antibody (5G4-2E9) [H00002937-M01] - GSS monoclonal antibody (M01), clone 5G4-2E9. Analysis of GSS expression in human colon.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-5G4-2E9-Western-Blot-H00002937-M01-img0002.jpg "Western Blot: Glutathione Synthetase Antibody (5G4-2E9) [H00002937-M01] - GSS monoclonal antibody (M01), clone 5G4-2E9. Analysis of GSS expression in human colon.")
![Sandwich ELISA: Human Glutathione Synthetase ELISA Kit (Colorimetric) [NBP3-06983] - Standard Curve Reference](http://images.novusbio.com/fullsize/Human-Glutathione-Synthetase-ELISA-Kit-Colorimetric-Sandwich-ELISA-NBP3-06983-img0001.jpg "Sandwich ELISA: Human Glutathione Synthetase ELISA Kit (Colorimetric) [NBP3-06983] - Standard Curve Reference")