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![Western Blot: FOXL2 Antibody [NB100-1277] - (1ug/ml) staining of Mouse Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/antibody/nb100-1277_goat-polyclonal-foxl2-antibody-imgenex-img-3228-197202382424.jpg "Western Blot: FOXL2 Antibody [NB100-1277] - (1ug/ml) staining of Mouse Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: FOXL2 Antibody [NB100-1277] - Analysis of FOXL2 in PFA-fixed, E14.5 mouse ovary section using anti-FOXL2 antibody (magenta), germ cell marker (green) and hoechst (grey). ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunofluorescence-NB100-1277-img0020.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - (8ug/ml) staining of paraffin embedded Human Ovary. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/antibody/nb100-1277_goat-polyclonal-foxl2-antibody-imgenex-img-3228-immunohistochemistry-paraffin-197202382651..jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody [NB100-1277] - Negative Control showing staining of paraffin embedded Human Ovary, with no primary antibody.](http://images.novusbio.com/fullsize/antibody/nb100-1277_goat-polyclonal-foxl2-antibody-imgenex-img-3228-immunohistochemistry-paraffin-19720238287..jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody [NBP2-70013] - Analysis of a FFPE tissue section of the human ovary using 1:200 dilution of FOXL2 antibody (NBP2-70013). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a nuclear staining with weak cytoplasmic positivity.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunohistochemistry-Paraffin-NBP2-70013-img0003.jpg "Immunohistochemistry-Paraffin: FOXL2 Antibody [NBP2-70013] - Analysis of a FFPE tissue section of the human ovary using 1:200 dilution of FOXL2 antibody (NBP2-70013). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a nuclear staining with weak cytoplasmic positivity.")
![Western Blot: FOXL2 Antibody [NBP2-70013] - Total protein from K562 and human testis was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-FOXL2 in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Western-Blot-NBP2-70013-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: FOXL2 Antibody [NBP2-70013] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-FOXL2 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-70013-img0006.jpg)
![Flow Cytometry: FOXL2 Antibody [NBP2-70013] - An intracellular stain was performed on Jurkat cells with NBP2-70013 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Flow-Cytometry-NBP2-70013-img0005.jpg)
![Flow Cytometry: FOXL2 Antibody [NBP2-70013] - An intracellular stain was performed on HeLa cells with NBP2-70013 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Flow-Cytometry-NBP2-70013-img0002.jpg)
![Western Blot: FOXL2 Antibody (CL12753) [NBP3-18569] - Analysis in human cell line K562.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12753-Western-Blot-NBP3-18569-img0008.jpg "Western Blot: FOXL2 Antibody (CL12753) [NBP3-18569] - Analysis in human cell line K562.")
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12753) [NBP3-18569] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12753-Immunohistochemistry-Paraffin-NBP3-18569-img0007.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12753) [NBP3-18569] - Analysis in human ovary and liver tissues using NBP3-18569 antibody. Corresponding FOXL2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12753-Immunohistochemistry-Paraffin-NBP3-18569-img0001.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12753) [NBP3-18569] - Staining of human ovarian cancer (granulosa cell tumour) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12753-Immunohistochemistry-Paraffin-NBP3-18569-img0002.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12753) [NBP3-18569] - Staining of human ovarian cancer (sex cord tumor) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12753-Immunohistochemistry-Paraffin-NBP3-18569-img0003.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12753) [NBP3-18569] - Staining of human ovary shows moderate to strong nuclear positivity in follicle (granulosa) cells.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12753-Immunohistochemistry-Paraffin-NBP3-18569-img0004.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12753) [NBP3-18569] - Staining of human fallopian tube shows moderate nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12753-Immunohistochemistry-Paraffin-NBP3-18569-img0005.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12753) [NBP3-18569] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12753-Immunohistochemistry-Paraffin-NBP3-18569-img0006.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12762) [NBP3-18544] - Staining of human rectum shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12762-Immunohistochemistry-Paraffin-NBP3-18544-img0006.jpg "Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12762) [NBP3-18544] - Staining of human rectum shows no positivity in glandular cells as expected.")
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12762) [NBP3-18544] - Analysis in human ovary and liver tissues using NBP3-18544 antibody. Corresponding FOXL2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12762-Immunohistochemistry-Paraffin-NBP3-18544-img0001.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12762) [NBP3-18544] - Staining of human ovarian cancer (granulosa cell tumor) shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12762-Immunohistochemistry-Paraffin-NBP3-18544-img0002.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12762) [NBP3-18544] - Staining of human ovarian cancer (sex cord tumor) shows moderate nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12762-Immunohistochemistry-Paraffin-NBP3-18544-img0003.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12762) [NBP3-18544] - Staining of human ovary shows moderate nuclear positivity in follicle (granulosa) cells.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12762-Immunohistochemistry-Paraffin-NBP3-18544-img0004.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12762) [NBP3-18544] - Staining of human fallopian tube shows weak to moderate nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12762-Immunohistochemistry-Paraffin-NBP3-18544-img0005.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody (CL12762) [NBP3-18544] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/FOXL2-Antibody-CL12762-Immunohistochemistry-Paraffin-NBP3-18544-img0007.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody [NBP2-49608] - Staining in human ovary and skeletal muscle tissues.. Corresponding FOXL2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunohistochemistry-Paraffin-NBP2-49608-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FOXL2 Antibody [NBP2-49608] - Staining in human ovary and skeletal muscle tissues.. Corresponding FOXL2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: FOXL2 Antibody [NBP2-49608] - Immunofluorescent staining of human cell line SiHa shows localization to nucleus & cytokinetic bridge. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49608-img0003.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody [NBP2-49608] - Staining of human fallopian tube shows moderate nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunohistochemistry-Paraffin-NBP2-49608-img0011.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody [NBP2-49608] - Staining of human ovary shows moderate nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunohistochemistry-Paraffin-NBP2-49608-img0008.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody [NBP2-49608] - Staining of human skeletal muscle shows no positivity in myocytes.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunohistochemistry-Paraffin-NBP2-49608-img0009.jpg)
![Immunohistochemistry-Paraffin: FOXL2 Antibody [NBP2-49608] - Staining of human endometrium shows weak nuclear positivity in cells in endometrial stroma.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunohistochemistry-Paraffin-NBP2-49608-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: FOXL2 Antibody [NBP2-22473] - Staining in KK1 granulosa cells.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22473-img0003.jpg "Immunocytochemistry/Immunofluorescence: FOXL2 Antibody [NBP2-22473] - Staining in KK1 granulosa cells.")
![Immunohistochemistry-Paraffin: FOXL2 Antibody [NBP2-22473] - Staining of FoxL2 in murine prenatal follicle.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunohistochemistry-Paraffin-NBP2-22473-img0004.jpg)
![Western Blot: FOXL2 Antibody [NBP3-03941] - Western blot analysis of extracts of various cell lines, using (NBP3-03941) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/FOXL2 Antibody-Western Blot-NBP3-03941-img0003.jpg "Western Blot: FOXL2 Antibody [NBP3-03941] - Western blot analysis of extracts of various cell lines, using (NBP3-03941) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry: FOXL2 Antibody [NBP3-03941] - Immunofluorescence analysis of mouse ovary using FOXL2 Rabbit pAb (NBP3-03941) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunohistochemistry-NBP3-03941-img0002.jpg)
![Immunohistochemistry: FOXL2 Antibody [NBP3-03941] - Immunofluorescence analysis of rat ovary using FOXL2 Rabbit pAb (NBP3-03941) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FOXL2-Antibody-Immunohistochemistry-NBP3-03941-img0001.jpg)
![Western Blot: FOXL2 Antibody (JE38-52) [NBP3-32348] - Western blot analysis of FOXL2 on different lysates with Rabbit anti-FOXL2 antibody (NBP3-32348) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 39 kDa Observed band size: 45 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32348) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32348_rabbit-foxl2-mab-je38-52-27820241633124.jpg "Western Blot: FOXL2 Antibody (JE38-52) [NBP3-32348] - Western blot analysis of FOXL2 on different lysates with Rabbit anti-FOXL2 antibody (NBP3-32348) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 39 kDa Observed band size: 45 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32348) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FOXL2 Antibody (JE38-52) [NBP3-32348] - Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-FOXL2 antibody (NBP3-32348) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32348) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32348_rabbit-foxl2-mab-je38-52-29820241492698.jpg)
![Western Blot: FOXL2 Antibody (4H5X3) [NBP3-16231] - Western blot analysis of extracts of various cell lines, using FOXL2 Rabbit mAb (NBP3-16231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/FOXL2-Antibody-4H5X3-Western-Blot-NBP3-16231-img0001.jpg "Western Blot: FOXL2 Antibody (4H5X3) [NBP3-16231] - Western blot analysis of extracts of various cell lines, using FOXL2 Rabbit mAb (NBP3-16231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: FOXL2 Antibody (4H5X3) [NBP3-16231] - Western blot analysis of extracts of various cell lines, using FOXL2 Rabbit mAb (NBP3-16231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/FOXL2-Antibody-4H5X3-Western-Blot-NBP3-16231-img0002.jpg)
