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![Western Blot: eIF4A1 Antibody [NBP2-24632] - Representative immunoblot of total lysate of MEFs with eIF4A (C-terminal epitope) and Tubulin antibodies in control and GS (glucose starvation). Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/21907) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/eIF4A1-Antibody-Western-Blot-NBP2-24632-img0003.jpg "Western Blot: eIF4A1 Antibody [NBP2-24632] - Representative immunoblot of total lysate of MEFs with eIF4A (C-terminal epitope) and Tubulin antibodies in control and GS (glucose starvation). Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/21907) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-24632] - Analysis of eIF4A1 in human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/eIF4A1-Antibody-Immunohistochemistry-Paraffin-NBP2-24632-img0001.jpg)
![Western Blot: eIF4A1 Antibody [NBP2-24632] - Analysis of eIF4A1 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) RAW cell lysate in the absence of immunizing peptide using NBP2-24632 at 0.25 ug/ml.](http://images.novusbio.com/fullsize/eIF4A1-Antibody-Western-Blot-NBP2-24632-img0002.jpg)
![Western Blot: eIF4A1 Antibody [NBP3-04718] - Western blot analysis of extracts of various cell lines, using eIF4A1 antibody (NBP3-04718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/eIF4A1 Antibody-Western Blot-NBP3-04718-img0005.jpg "Western Blot: eIF4A1 Antibody [NBP3-04718] - Western blot analysis of extracts of various cell lines, using eIF4A1 antibody (NBP3-04718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody - Azide and BSA Free [eIF4A1] - Immunofluorescence analysis of U2OS cells using eIF4A1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04718_rabbit-polyclonal-eif4a1-antibody-52202516345225.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody - Azide and BSA Free [eIF4A1] - Immunofluorescence analysis of C6 cells using eIF4A1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04718_rabbit-polyclonal-eif4a1-antibody-52202516321452.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody - Azide and BSA Free [eIF4A1] - Immunofluorescence analysis of U2OS cells using eIF4A1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04718_rabbit-polyclonal-eif4a1-antibody-52202516293927.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody - Azide and BSA Free [eIF4A1] - Immunofluorescence analysis of NIH/3T3 cells using eIF4A1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04718_rabbit-polyclonal-eif4a1-antibody-522025163961.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody - Azide and BSA Free [eIF4A1] - Immunofluorescence analysis of C6 cells using eIF4A1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04718_rabbit-polyclonal-eif4a1-antibody-52202516293958.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody - Azide and BSA Free [eIF4A1] - Immunofluorescence analysis of NIH/3T3 cells using eIF4A1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-04718_rabbit-polyclonal-eif4a1-antibody-5220251638073.jpg)
![Western Blot: eIF4A1 Antibody (JE35-43) [NBP3-32299] - Western blot analysis of eIF4A1 on different lysates with Rabbit anti-eIF4A1 antibody (NBP3-32299) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: K-562 cell lysate Lane 4: HEK-293 cell lysate Lane 5: MCF7 cell lysate Lane 6: HepG2 cell lysate Lane 7: Huh7 cell lysate Lane 8: Jurkat cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32299) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32299_rabbit-eif4a1-mab-je35-43-278202415212440.jpg "Western Blot: eIF4A1 Antibody (JE35-43) [NBP3-32299] - Western blot analysis of eIF4A1 on different lysates with Rabbit anti-eIF4A1 antibody (NBP3-32299) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: K-562 cell lysate Lane 4: HEK-293 cell lysate Lane 5: MCF7 cell lysate Lane 6: HepG2 cell lysate Lane 7: Huh7 cell lysate Lane 8: Jurkat cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32299) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: eIF4A1 Antibody (JE35-43) [NBP3-32299] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-eIF4A1 antibody (NBP3-32299) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32299) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32299_rabbit-eif4a1-mab-je35-43-298202413585597.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4A1 Antibody (JE35-43) [NBP3-32299] - Immunocytochemistry analysis of HeLa cells labeling eIF4A1 with Rabbit anti-eIF4A1 antibody (NBP3-32299) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-eIF4A1 antibody (NBP3-32299) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32299_rabbit-eif4a1-mab-je35-43-29202410334068.jpg)
![Flow Cytometry: eIF4A1 Antibody (JE35-43) [NBP3-32299] - Flow cytometric analysis of HeLa cells labeling eIF4A1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32299, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32299_rabbit-eif4a1-mab-je35-43-392024884657.jpg)
![Western Blot: eIF4A1 Antibody [NBP2-16302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF4A1 antibody diluted by 1:3000.](http://images.novusbio.com/fullsize/eIF4A1-Antibody-Western-Blot-NBP2-16302-img0007.jpg "Western Blot: eIF4A1 Antibody [NBP2-16302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF4A1 antibody diluted by 1:3000.")
![Immunocytochemistry/Immunofluorescence: eIF4A1 Antibody [NBP2-16302] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/eIF4A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16302-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF4A1 Antibody [NBP2-16302] - Human colon carcinoma, using EIF4A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/eIF4A1-Antibody-Immunohistochemistry-Paraffin-NBP2-16302-img0002.jpg)
![Immunoprecipitation: eIF4A1 Antibody [NBP2-16302] - HepG2 whole cell extracts using 5 ug of EIF4A1 antibody. Western blot analysis was performed using EIF4A1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/eIF4A1-Antibody-Immunoprecipitation-NBP2-16302-img0006.jpg)
![Western Blot: eIF4A1 Antibody (7C3) [NBP3-15220] - Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, RAW264.7 whole cell lysate. All lanes: eIF4A1 Antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 47 KDa. Observed band size: 50 KDa.](http://images.novusbio.com/fullsize/eIF4A1-Antibody-7C3-Western-Blot-NBP3-15220-img0002.jpg "Western Blot: eIF4A1 Antibody (7C3) [NBP3-15220] - Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, RAW264.7 whole cell lysate. All lanes: eIF4A1 Antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 47 KDa. Observed band size: 50 KDa.")
![Immunocytochemistry/Immunofluorescence: eIF4A1 Antibody (7C3) [NBP3-15220] - Immunofluorescence staining of Hela Cells with at eIF4A1 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/eIF4A1-Antibody-7C3-Immunocytochemistry-Immunofluorescence-NBP3-15220-img0001.jpg)
![Western Blot: eIF4A1 Antibody (SR1793) [NBP3-21969] - Western blot analysis using NBP3-21969 in MCF7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21969_rabbit-eif4a1-mab-sr1793-6720239533710.jpg "Western Blot: eIF4A1 Antibody (SR1793) [NBP3-21969] - Western blot analysis using NBP3-21969 in MCF7 cell lysate.")
![Western Blot: eIF4A1 Overexpression Lysate (Adult Normal) [NBL1-10201] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF4A1.](http://images.novusbio.com/fullsize/eIF4A1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10201-img0002.jpg "Western Blot: eIF4A1 Overexpression Lysate (Adult Normal) [NBL1-10201] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF4A1.")