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![Immunohistochemistry-Paraffin: PERK Antibody [NBP1-80930] - Analysis in human parathyroid gland and liver tissues using NBP1-80930 antibody. Corresponding EIF2AK3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-Paraffin-NBP1-80930-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PERK Antibody [NBP1-80930] - Analysis in human parathyroid gland and liver tissues using NBP1-80930 antibody. Corresponding EIF2AK3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PERK Antibody [NBP1-80930] - Staining of human Colon shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-Paraffin-NBP1-80930-img0011.jpg)
![Immunohistochemistry-Paraffin: PERK Antibody [NBP1-80930] - Staining of human parathyroid gland shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-Paraffin-NBP1-80930-img0012.jpg)
![Immunohistochemistry-Paraffin: PERK Antibody [NBP1-80930] - Staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-Paraffin-NBP1-80930-img0013.jpg)
![Immunohistochemistry-Paraffin: PERK Antibody [NBP1-80930] - Staining of human prostate shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-Paraffin-NBP1-80930-img0014.jpg)
![Immunohistochemistry-Paraffin: PERK Antibody [NBP1-80930] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-Paraffin-NBP1-80930-img0015.jpg)
![Western Blot: PERK Antibody [NBP1-78017] - PERK in cell lysates. 300ug PERK over-expressing 293T cell lysate (lanes 1 & 2), or 800ug wild type (Lanes 3 & 4), and PERK knock out (lanes 5 & 6) MEF cell lysate were immunoprecipitated with 15ul anti-PERK, followed by western blotIgG with 1:1000 dilution of anti-PERK in 5% milk/TBST buffer. Lane 1, 293T cells over-expressing Myc-PERK wt, Lane 2, 293T cells over-expressing Myc-PERK K618A. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.](http://images.novusbio.com/fullsize/PERK Antibody-Western Blot-NBP1-78017-img0011.jpg "Western Blot: PERK Antibody [NBP1-78017] - PERK in cell lysates. 300ug PERK over-expressing 293T cell lysate (lanes 1 & 2), or 800ug wild type (Lanes 3 & 4), and PERK knock out (lanes 5 & 6) MEF cell lysate were immunoprecipitated with 15ul anti-PERK, followed by western blotIgG with 1:1000 dilution of anti-PERK in 5% milk/TBST buffer. Lane 1, 293T cells over-expressing Myc-PERK wt, Lane 2, 293T cells over-expressing Myc-PERK K618A. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: PERK Antibody [NBP1-78017] - Analysis of mouse mammary gland samples from lactating mice (L10) with anti-PERK. Positive staining signal observed in wild type mouse sample with anti-PERK staining only (middle image), but not in the knock out mouse sample (right image) and pre-immune serum staining (left image) The anti-PERK was diluted 1:1,000 in 5% goat serum in PBS and allowed to incubate for 2h at room temperature in a humidified chamber](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-NBP1-78017-img0010.jpg)
![Western Blot: PERK Antibody [NBP3-12891] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with PERK antibody (NBP3-12891) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PERK-Antibody-Western-Blot-NBP3-12891-img0001.jpg "Western Blot: PERK Antibody [NBP3-12891] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with PERK antibody (NBP3-12891) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PERK Antibody [NBP3-12891] - Rat brain. PERK stained by PERK antibody (NBP3-12891) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-Paraffin-NBP3-12891-img0003.jpg)
![Immunohistochemistry-Paraffin: PERK Antibody [NBP3-12891] - Mouse brain. PERK stained by PERK antibody (NBP3-12891) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-Paraffin-NBP3-12891-img0002.jpg)
![Western Blot: PERK Antibody [NBP3-12193] - WB of NBP3-12193 with adrenal cells. Left-Chicken serum antibodies. Right- Total NBP3-12193 IGY. Apparent MW is 135 kDa.](http://images.novusbio.com/fullsize/PERK-Antibody-Western-Blot-NBP3-12193-img0001.jpg "Western Blot: PERK Antibody [NBP3-12193] - WB of NBP3-12193 with adrenal cells. Left-Chicken serum antibodies. Right- Total NBP3-12193 IGY. Apparent MW is 135 kDa.")
![Immunohistochemistry-Paraffin: PERK Antibody [NBP3-12193] - Mouse cerebellum. 1:100 in IHC blobking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification on Leica 40X DM4000B. FFPE section.](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-Paraffin-NBP3-12193-img0002.jpg)
![Immunohistochemistry-Paraffin: PERK Antibody [NBP3-12194] - 1:100 dilution in IHC Blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE.](http://images.novusbio.com/fullsize/PERK-Antibody-Immunohistochemistry-Paraffin-NBP3-12194-img0001.jpg "Immunohistochemistry-Paraffin: PERK Antibody [NBP3-12194] - 1:100 dilution in IHC Blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE.")
![Western Blot: PERK Antibody (5G5) [NBP1-51661] - Western blot analysis using EIF2AK3 mAb against HEK293 (1) and EIF2AK3-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PERK-Antibody-5G5-Western-Blot-NBP1-51661-img0001.jpg "Western Blot: PERK Antibody (5G5) [NBP1-51661] - Western blot analysis using EIF2AK3 mAb against HEK293 (1) and EIF2AK3-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: PERK Overexpression Lysate (Adult Normal) [NBL1-10177] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PERK.](http://images.novusbio.com/fullsize/PERK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10177-img0002.jpg "Western Blot: PERK Overexpression Lysate (Adult Normal) [NBL1-10177] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PERK.")
![Sandwich ELISA: PERK Antibody (7B10) [H00009451-M03-100ug] - Detection limit for recombinant GST tagged EIF2AK3 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PERK-Antibody-7B10-Sandwich-ELISA-H00009451-M03-100ug-img0001.jpg "Sandwich ELISA: PERK Antibody (7B10) [H00009451-M03-100ug] - Detection limit for recombinant GST tagged EIF2AK3 is 0.03 ng/ml as a capture antibody.")
![Western Blot: PERK Antibody (PSH0-81) [NBP3-32738] - Western blot analysis of PERK on different lysates with Rabbit anti-PERK antibody (NBP3-32738) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 6: U-87 MG cell lysate (20 ug/Lane) Lane 7: K-562 cell lysate (16 ug/Lane) Predicted band size: 125 kDa Observed band size: 140 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32738) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32738_rabbit-perk-mab-psh0-81-278202416285261.jpg "Western Blot: PERK Antibody (PSH0-81) [NBP3-32738] - Western blot analysis of PERK on different lysates with Rabbit anti-PERK antibody (NBP3-32738) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 6: U-87 MG cell lysate (20 ug/Lane) Lane 7: K-562 cell lysate (16 ug/Lane) Predicted band size: 125 kDa Observed band size: 140 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32738) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")