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![Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.](http://images.novusbio.com/fullsize/DIO3-Antibody-Western-Blot-NBP1-05767-img0002.jpg "Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.")
![Flow Cytometry: DIO3 Antibody [NBP1-05767] - Analysis using the Biotin conjugate of NBP1-05767. Dio3 staining of mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.](http://images.novusbio.com/fullsize/DIO3-Antibody-Flow-Cytometry-NBP1-05767-img0005.jpg)
![Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rabbit brain whole cell homogenate. Photo courtesy of product review by verified customer.](http://images.novusbio.com/fullsize/DIO3-Antibody-Western-Blot-NBP1-05767-img0003.jpg)
![Western Blot: DIO3 Antibody [NB110-96414] - Detection of Dio3 in rat placenta](http://images.novusbio.com/fullsize/DIO3-Antibody-Western-Blot-NB110-96414-img0002.jpg "Western Blot: DIO3 Antibody [NB110-96414] - Detection of Dio3 in rat placenta")
![Immunohistochemistry-Paraffin: DIO3 Antibody [NB110-96414] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human placenta using 1:50 dilution of DIO3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in decidual cells was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/DIO3-Antibody-Immunohistochemistry-Paraffin-NB110-96414-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DIO3 Antibody [NBP3-17201] - Staining of human cell line SH-SY5Y shows localization to vesicles.](http://images.novusbio.com/fullsize/DIO3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17201-img0001.jpg "Immunocytochemistry/Immunofluorescence: DIO3 Antibody [NBP3-17201] - Staining of human cell line SH-SY5Y shows localization to vesicles.")
![Western Blot: DIO3 Antibody [NBP2-86616] - Host: Rabbit. Target Name: DIO3. Sample Tissue: Human MCF7 Whole Cell. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/DIO3-Antibody-Western-Blot-NBP2-86616-img0001.jpg "Western Blot: DIO3 Antibody [NBP2-86616] - Host: Rabbit. Target Name: DIO3. Sample Tissue: Human MCF7 Whole Cell. Antibody Dilution: 1.0ug/ml")
![Western Blot: DIO3 Antibody [NBP2-92482] - Analysis of extracts of various cell lines, using DIO3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/DIO3-Antibody-Western-Blot-NBP2-92482-img0001.jpg "Western Blot: DIO3 Antibody [NBP2-92482] - Analysis of extracts of various cell lines, using DIO3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")