Hu,
Mu,
Rt,
Po,
Bv,
Pm,
RbSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Bv, Pm, Rb
WB, Simple Western, Flow, GS, ICC/IF, IHC, IP
Applications: WB, Simple Western, Flow, GS, ICC/IF, IHC, IP
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: CUGBP1 Antibody (3B1) [NB200-316] - Detection of CUG-BP1 in several cell lysates.](http://images.novusbio.com/fullsize/CUGBP1-Antibody-3B1-Western-Blot-NB200-316-img0007.jpg "Western Blot: CUGBP1 Antibody (3B1) [NB200-316] - Detection of CUG-BP1 in several cell lysates.")
![Immunocytochemistry/Immunofluorescence: CUGBP1 Antibody (3B1) [NB200-316] - Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts.](http://images.novusbio.com/fullsize/CUGBP1-Antibody-3B1-Immunocytochemistry-Immunofluorescence-NB200-316-img0006.jpg)
![Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Nuclear staining was observed in lymphocytes.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-3B1-Immunohistochemistry-Paraffin-NB200-316-img0010.jpg)
![Flow Cytometry: CUGBP1 Antibody (3B1) [NB200-316] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using CUGBP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/CUGBP1-Antibody-3B1-Flow-Cytometry-NB200-316-img0008.jpg)
![Simple Western: CUGBP1 Antibody (3B1) [NB200-316] - Simple Western lane view shows a specific band for CUGBP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/CUGBP1-Antibody-3B1-Simple-Western-NB200-316-img0009.jpg)
![Western Blot: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUGBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUGBP1.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Western-Blot-NBP2-02343-img0001.jpg "Western Blot: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUGBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUGBP1.")
![Immunocytochemistry/Immunofluorescence: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CUGBP1.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Immunocytochemistry-Immunofluorescence-NBP2-02343-img0009.jpg)
![Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded prostate tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Immunohistochemistry-Paraffin-NBP2-02343-img0008.jpg)
![Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Immunohistochemistry-Paraffin-NBP2-02343-img0002.jpg)
![Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Immunohistochemistry-Paraffin-NBP2-02343-img0003.jpg)
![Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Immunohistochemistry-Paraffin-NBP2-02343-img0004.jpg)
![Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded Kidney tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Immunohistochemistry-Paraffin-NBP2-02343-img0005.jpg)
![Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded liver tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Immunohistochemistry-Paraffin-NBP2-02343-img0006.jpg)
![Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (OTI5B8) [NBP2-02343] - Staining of paraffin-embedded pancreas tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Immunohistochemistry-Paraffin-NBP2-02343-img0007.jpg)
![Western Blot: CUGBP1/CELF1 Antibody (OTI5B8) - Azide and BSA Free [NBP2-71623] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUGBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUGBP1.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Azide-and-BSA-Free-Western-Blot-NBP2-71623-img0001.jpg "Western Blot: CUGBP1/CELF1 Antibody (OTI5B8) - Azide and BSA Free [NBP2-71623] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CUGBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CUGBP1.")
![Immunocytochemistry/Immunofluorescence: CUGBP1/CELF1 Antibody (OTI5B8) - Azide and BSA Free [NBP2-71623] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CUGBP1.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71623-img0002.jpg)
![Immunohistochemistry: CUGBP1/CELF1 Antibody (OTI5B8) - Azide and BSA Free [NBP2-71623] - Staining of paraffin-embedded pancreas tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71623-img0008.jpg)
![Flow Cytometry: CUGBP1/CELF1 Antibody (OTI5B8) - Azide and BSA Free [NBP2-71623] - Staining of paraffin-embedded prostate tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71623-img0009.jpg)
![Immunohistochemistry: CUGBP1/CELF1 Antibody (OTI5B8) - Azide and BSA Free [NBP2-71623] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71623-img0003.jpg)
![Immunohistochemistry: CUGBP1/CELF1 Antibody (OTI5B8) - Azide and BSA Free [NBP2-71623] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71623-img0004.jpg)
![Immunohistochemistry: CUGBP1/CELF1 Antibody (OTI5B8) - Azide and BSA Free [NBP2-71623] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71623-img0005.jpg)
![Immunohistochemistry: CUGBP1/CELF1 Antibody (OTI5B8) - Azide and BSA Free [NBP2-71623] - Staining of paraffin-embedded Kidney tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71623-img0006.jpg)
![Immunohistochemistry: CUGBP1/CELF1 Antibody (OTI5B8) - Azide and BSA Free [NBP2-71623] - Staining of paraffin-embedded liver tissue using anti-CUGBP1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-OTI5B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71623-img0007.jpg)
![Western Blot: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179] - Western blot analysis of CUGBP1/CELF1 on different lysates with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: NCI-H226 cell lysate Lane 4: COS-1 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32179) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32179_mouse-cugbp1-celf1-mab-7a1-r-27820241529558.jpg "Western Blot: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179] - Western blot analysis of CUGBP1/CELF1 on different lysates with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: NCI-H226 cell lysate Lane 4: COS-1 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: NIH/3T3 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32179) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32179) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32179_mouse-cugbp1-celf1-mab-7a1-r-298202414115427.jpg)
![Immunocytochemistry/ Immunofluorescence: CUGBP1/CELF1 Antibody (7A1-R) [NBP3-32179] - Immunocytochemistry analysis of HeLa cells labeling CUGBP1/CELF1 with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CUGBP1/CELF1 antibody (NBP3-32179) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32179_mouse-cugbp1-celf1-mab-7a1-r-2920241192378.jpg)
![Western Blot: CUGBP1/CELF1 Antibody (SR1914) [NBP3-22304] - Western blot analysis using NBP3-22304 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22304_rabbit-cugbp1-celf1-mab-sr1914-672023106261.jpg "Western Blot: CUGBP1/CELF1 Antibody (SR1914) [NBP3-22304] - Western blot analysis using NBP3-22304 in HeLa cell lysate.")
![Western Blot: CUGBP1/CELF1 Antibody [NBP2-76536] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-Western-Blot-NBP2-76536-img0001.jpg "Western Blot: CUGBP1/CELF1 Antibody [NBP2-76536] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: CUGBP1/CELF1 Antibody [NBP2-76536] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76536-img0002.jpg)
![Western Blot: CUGBP1/CELF1 Antibody (2B2P2) [NBP3-15394] - Western blot analysis of extracts of SH-SY5Y cells, using CUGBP1/CELF1 Rabbit mAb (NBP3-15394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-2B2P2-Western-Blot-NBP3-15394-img0001.jpg "Western Blot: CUGBP1/CELF1 Antibody (2B2P2) [NBP3-15394] - Western blot analysis of extracts of SH-SY5Y cells, using CUGBP1/CELF1 Rabbit mAb (NBP3-15394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: CUGBP1/CELF1 Antibody (2B2P2) [NBP3-15394] - Western blot analysis of extracts of various cell lines, using CUGBP1/CELF1 Rabbit mAb (NBP3-15394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/CUGBP1-CELF1-Antibody-2B2P2-Western-Blot-NBP3-15394-img0002.jpg)
![Western Blot: CUGBP1 Overexpression Lysate (Adult Normal) [NBL1-09605] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CUGBP1.](http://images.novusbio.com/fullsize/CUGBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09605-img0002.jpg "Western Blot: CUGBP1 Overexpression Lysate (Adult Normal) [NBL1-09605] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CUGBP1.")
![Western Blot: CUGBP1 Overexpression Lysate (Adult Normal) [NBL1-09604] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CUGBP1.](http://images.novusbio.com/fullsize/CUGBP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09604-img0002.jpg "Western Blot: CUGBP1 Overexpression Lysate (Adult Normal) [NBL1-09604] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CUGBP1.")