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NBP2-27398

BAG3 Antibody

(13 Publications)

0.05 ml

NBP2-27398

Simple Western: BAG3 Antibody [NBP2-27398] - Simple Western lane view shows a specific band for BAG3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

<b>Biological Strategies Validation. </b>Immunohistochemistry: BAG3 Antibody [NBP2-27398] - IHC of BAG3 in IR myocardium. BAG3 expression is significantly decreased in the STZ group than those in the CON group. BAG3 expression is also significantly reduced in the IR CON and IR STZ groups as compared with the CON group. IR STZ hearts further depress BAG3 expression than IR CON hearts. Data are expressed as mean +/- SEM (n = 3) using the single values. * p < 0.05 vs. CON, a p < 0.05 vs. STZ, b p < 0.05 vs. IR CON. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2076-3921/9/8/679) licensed under a CC-BY license.

<b>Biological Strategies Validation. </b>Western Blot: BAG3 Antibody [NBP2-27398] - Western blot of BAG3 in IR myocardium. BAG3 expression is significantly decreased in the STZ group than those in the CON group. BAG3 expression is also significantly reduced in the IR CON and IR STZ groups as compared with the CON group. IR STZ hearts further depress BAG3 expression than IR CON hearts. Data are expressed as mean +/- SEM (n = 3) using the single values. * p < 0.05 vs. CON, a p < 0.05 vs. STZ, b p < 0.05 vs. IR CON. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2076-3921/9/8/679) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP2-27398] - Antibody was tested in A431 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Western Blot: BAG3 Antibody [NBP2-27398] - Analysis of BAG3 using NBP2-27398 at 1:2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.

Immunohistochemistry-Frozen: BAG3 Antibody [NBP2-27398] - Frozen mouse muscle tissue section stained for BAG-3 expression using NBP2-27398 at 1:2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG-3 localizes with Z-disk proteins.

Mesenteric vascular Western blot graph (A) and analysis of 4-HNE (B), CaSR (C), p-eNOS (D) and BAG3 (E) are demonstrated. Microvascular reactivity from mesenteric arterioles to a vasoconstrictor (norepinephrine, NE, F) and vasodilator (acetylcholine, Ach, G) is displayed in CON and STZ groups. All data are presented as the mean ± SEM (n = 3) using the single values in each test. * p < 0.05 vs. CON group.

Western Blot: BAG3 Antibody [NBP2-27398] - Mesenteric vascular Western blot graph (A) & analysis of 4-HNE (B), CaSR (C), p-eNOS (D) & BAG3 (E) are demonstrated. Microvascular reactivity from mesenteric arterioles to a vasoconstrictor (norepinephrine, NE, F) & vasodilator (acetylcholine, Ach, G) is displayed in CON & STZ groups. All data are presented as the mean ± SEM (n = 3) using the single values in each test. * p < 0.05 vs. CON group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32751309), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5676

Alt Catalog# IMG-5676

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB100-56091

BAG5 Antibody

(3 Publications)

0.05 ml

NB100-56091

Immunohistochemistry-Paraffin: BAG5 Antibody [NB100-56091] - Formalin-fixed, paraffin-embedded human cervical tissue stained for BAG-5 expression using this antibody at 1:2000. A. normal cervix epithelium. B. cervical cancer. Hematoxylin-eosin counterstain.

Immunohistochemistry-Paraffin: BAG5 Antibody [NB100-56091] - Formalin-fixed, paraffin-embedded human tissues stained for BAG-5 expression using this antibody at 1:2000. A. normal placenta. B. breast cancer. C. normal spleen. normal cervix epithelium. Hematoxylin-eosin counterstain.

Reactivity:

Hu, Mu, Rt, Bv, Ca, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Ch

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5678

Alt Catalog# IMG-5678

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NB100-56087

BAG2 Antibody

(3 Publications)

0.05 ml

NB100-56087

Western Blot: BAG2 Antibody [NB100-56087] - analysis of BAG-2 using this antibody. HeLa stably transfected Myc-tagged CHIP (<Carboxyl terminus of Hsp70-interacting protein) were immunoprecipitated with an anti-Myc antibody. Immunocomplexes were resolved by SDS-PAGE followed by analysis of BAG-2. This experiment was done to show the colocalization of CHIP and Bag-2. Please refer to Dai et al for additional information on HeLa cells stably transfected with Myc-CHIP.

<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BAG2 Antibody [NB100-56087] - Immunofluorescence confocal microscopy of BAG-2 using NB100-56087 at 1:2000. HeLa cells stably transfected with Myc-CHIP (carboxyl terminus of Hsp70-interacting protein) and growing on coverslips were left untreated (A) or heat shocked at 42 degrees for 30 min (B). Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG-2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock. Please refer to Dai et al (2005) for additional information on HeLa cells stably transfected with Myc-CHIP.

Immunohistochemistry-Paraffin: BAG2 Antibody [NB100-56087] - BAG-2 was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human BAG-2 polyclonal Antibody (Catalog # NB100-56087) at 1:1000 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5674

Alt Catalog# IMG-5674

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB100-56082

Bag-1 Antibody

(1 Publication)

0.05 ml

NB100-56082

Western Blot: Bag-1 Antibody [NB100-56082] - Analysis of BAG-1 in normal brain tissue lysates.

Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082] - Formalin-fixed, paraffin-embedded human breast tissue stained for BAG-1 expression using this antibody at 1:2000. A. normal breast epithelium. B. breast cancer. A1 and B1 are high magnifications from A and B, respectively. Hematoxylin-eosin counterstain.

Western Blot: Bag-1 Antibody [NB100-56082] - BAG-1 levels in human neuroblastoma. Image from verified customer review.

Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56082] - Formalin-fixed, paraffin-embedded mouse ischemic brain stained for BAG-1 expression using NB100-56082 at 1:2000. A1 is a higher magnification of A. Differential Bag-1 expression levels are seen in the hypothalmic nuclei. The variation in expression levels from lack of Bag-1 staining to strong staining may be linked to differential responses of the hypothalmic nuclei to ischemic stress. Hematoxylin-eosin counterstain.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5669

Alt Catalog# IMG-5669

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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H00000573-M02

Bag-1 Antibody (2D3)

(1 Publication)

0.1 mg

H00000573-M02

Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on HeLa cell. Antibody concentration 35 ug/ml.

Immunohistochemistry-Paraffin: Bag-1 Antibody (2D3) [H00000573-M02] - Analysis of monoclonal antibody to BAG1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.

Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - analysis of BAG1 expression in transfected 293T cell line by BAG1 monoclonal antibody (M02), clone 2D3. Lane 1: BAG1 transfected lysate (38.878 KDa). Lane 2: Non-transfected lysate.

Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3 Analysis of BAG1 expression in HeLa.

Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in PC-12.

Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in LNCaP.

Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in Raw 264.7.

Western Blot: Bag-1 Antibody (2D3) [H00000573-M02] - BAG1 monoclonal antibody (M02), clone 2D3. Analysis of BAG1 expression in Jurkat.

Sandwich ELISA: Bag-1 Antibody (2D3) [H00000573-M02] - Detection limit for recombinant GST tagged BAG1 is approximately 0.03ng/ml as a capture antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP1-86065

BAG5 Antibody

0.1 ml

NBP1-86065

Immunohistochemistry-Paraffin: BAG5 Antibody [NBP1-86065] - Staining in human testis and skeletal muscle tissues.. Corresponding BAG5 RNA-seq data are presented for the same tissues.

<b>Orthogonal Strategies Validation. </b>Western Blot: BAG5 Antibody [NBP1-86065] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/Immunofluorescence: BAG5 Antibody [NBP1-86065] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.

Western Blot: BAG5 Antibody [NBP1-86065] - Analysis in human cell line Daudi.

Immunohistochemistry-Paraffin: BAG5 Antibody [NBP1-86065] - Staining of human testis shows moderate positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: BAG5 Antibody [NBP1-86065] - Staining of human prostate shows very weak cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: BAG5 Antibody [NBP1-86065] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: BAG5 Antibody [NBP1-86065] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB100-56081

Bag-1 Antibody

(1 Publication)

0.05 ml

NB100-56081

Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56081] - Formalin-fixed, paraffin-embedded sections of human colorectal cancer stained for Bag-1 using this antibody at 1:2000. Hematoxylin-eosin counterstain. Samples from two patients (A and B) are shown. A1 and B1 are higher magnifications from A and B, respectively.

Immunohistochemistry-Paraffin: Bag-1 Antibody [NB100-56081] - Formalin-fixed, paraffin-embedded section of human colon stained for Bag-1 using this antibody at 1:2000. Hematoxylin-eosin counterstain.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-5668

Alt Catalog# IMG-5668

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-15543

BAG3 Antibody

(1 Review)

(1 Publication)

0.1 ml

NBP2-15543

Western Blot: BAG3 Antibody [NBP2-15543] - BAG3 antibody detects BAG3 protein by Western blot analysis. A. 50 ug mouse heart lysate/extract 7. 5 % SDS-PAGEBAG3 antibody dilution: 1:1000.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543] - BAG3 in high-grade serous carcinoma. 1:100 dilution, pH=6 retrieval buffer, Dako FLEX+ protocol. This image was submitted via customer Review.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.

Western Blot: BAG3 Antibody [NBP2-15543] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BAG3 antibody (NBP2-15543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Bv, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, RM

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-86429

BAG3 Antibody

0.1 ml

NBP1-86429

Western Blot: BAG3 Antibody [NBP1-86429] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Skeletal muscle tissue

Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP1-86429] - Staining of human cell line A-431 shows localization to plasma membrane & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human liver.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human kidney.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human testis.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebral cortex.

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebellum, prostate, skeletal muscle and skin using Anti-BAG3 antibody NBP1-86429 (A) shows similar protein distribution across tissues to independent antibody NBP1-86442 (B).

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86429] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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MAB852

Bag-1 Antibody (GP3.10G3E2) [Unconjugated]

(1 Publication)

100 μg

MAB852

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Bag-1 at approximately 36 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Bag-1 was immunoprecipitated from lysates (5 x 106cells) of THP-1 human acute monocytic leukemia and HEK293 human embryonic kidney cell line following incubation with 3 µg Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) for 1-16 hours at 4 °C. Bag-1-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bag-1 (left panel) was detected by Western blot using 0.5 µg/mL Mouse CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF852). View our recommended buffer recipes for immunoprecipitation.

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bag-1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Bag-1 at approximately 29, 39, and 50 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB852-SP

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NBP1-59087

BAG2 Antibody

(3 Publications)

100 ul

NBP1-59087

Western Blot: BAG2 Antibody [NBP1-59087] - Sample Tissue: Human HepG2 Antibody Dilution: 1.0 ug/ml

Immunohistochemistry-Paraffin: BAG2 Antibody [NBP1-59087] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.

Western Blot: BAG2 Antibody [NBP1-59087] - Lane 1: 20ug untransfected human Saos2 cells Lane 2: 20ug siRUVBL transfected human.

Western Blot: BAG2 Antibody [NBP1-59087] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-86442

BAG3 Antibody

(2 Publications)

0.1 ml

NBP1-86442

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human cerebellum, prostate, skeletal muscle and skin using Anti-BAG3 antibody NBP1-86442 (A) shows similar protein distribution across tissues to independent antibody NBP1-86429 (B).

<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: BAG3 Antibody [NBP1-86442] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP1-86442] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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