Hu,
Mu,
Rt,
Bv,
Ca,
Gp,
Pm,
Rb,
ShSpecies Glossary
Reactivity: Hu, Mu, Rt, Bv, Ca, Gp, Pm, Rb, Sh
WB, Simple Western, Flow, ICC/IF, IHC, B/N, Single-Cell Western
Applications: WB, Simple Western, Flow, ICC/IF, IHC, B/N, Single-Cell Western
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-XVIF9-G10-Immunocytochemistry-Immunofluorescence-NB300-540-img0009.jpg "Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in U251 glioma cells is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human prostate carcinoma tissues.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-XVIF9-G10-Immunohistochemistry-Paraffin-NB300-540-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-XVIF9-G10-Immunocytochemistry-Immunofluorescence-NB300-540-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in HeLa cells is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-XVIF9-G10-Immunocytochemistry-Immunofluorescence-NB300-540-img0007.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-XVIF9-G10-Immunohistochemistry-Paraffin-NB300-540-img0011.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Both normal and cancer biopsies of deparaffinized Human colon tissues.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-XVIF9-G10-Immunohistochemistry-Paraffin-NB300-540-img0012.jpg)
![Simple Western: Sodium Potassium ATPase Alpha 3 Antibody (XVIF9-G10) [NB300-540] - Simple Western lane view shows a specific band for Sodium Potassium ATPase Alpha 3 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-XVIF9-G10-Simple-Western-NB300-540-img0010.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-37955] - Analysis in human cerebral cortex and endometrium tissues. Corresponding ATP1A3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP2-37955-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-37955] - Analysis in human cerebral cortex and endometrium tissues. Corresponding ATP1A3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-37955] - Staining of human endometrium shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP2-37955-img0010.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-37955] - Staining of human cerebellum shows strong membranous positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP2-37955-img0008.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-37955] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP2-37955-img0009.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-37955] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP2-37955-img0011.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-37913] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP2-37913-img0004.jpg "Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-37913] - Staining of human endometrium shows low expression as expected.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-37913] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP2-37913-img0002.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-37913] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP2-37913-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-37913] - Staining in human cerebral cortex and endometrium tissues using anti-ATP1A3 antibody. Corresponding ATP1A3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP2-37913-img0005.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 3 Antibody (SR1002) [NBP3-22145] - Western blot analysis using NBP3-22145 in Rat brain lysate.](http://images.novusbio.com/fullsize/nbp3-22145_rabbit-sodium-potassium-atpase-alpha-3-mab-sr1002-57202315584830.jpg "Western Blot: Sodium Potassium ATPase Alpha 3 Antibody (SR1002) [NBP3-22145] - Western blot analysis using NBP3-22145 in Rat brain lysate.")
![Western Blot: Sodium Potassium ATPase Alpha 3 Antibody (JE35-24) [NBP3-32062] - Western blot analysis of Sodium Potassium ATPase Alpha 3 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 3 antibody (NBP3-32062) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse brain tissue lysate (70 heat) Lane 3: Mouse brain tissue lysate (no heat) Lane 4: Rat brain tissue lysate Lane 5: Rat brain tissue lysate (no heat) Lane 6: Mouse cerebellum tissue lysate Lane 7: Mouse cerebellum tissue lysate (70 heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 112 kDa Observed band size: 100/150 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32062) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32062_rabbit-sodium-potassium-atpase-alpha-3-mab-je35-24-278202415295536.jpg "Western Blot: Sodium Potassium ATPase Alpha 3 Antibody (JE35-24) [NBP3-32062] - Western blot analysis of Sodium Potassium ATPase Alpha 3 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 3 antibody (NBP3-32062) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse brain tissue lysate (70 heat) Lane 3: Mouse brain tissue lysate (no heat) Lane 4: Rat brain tissue lysate Lane 5: Rat brain tissue lysate (no heat) Lane 6: Mouse cerebellum tissue lysate Lane 7: Mouse cerebellum tissue lysate (70 heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 112 kDa Observed band size: 100/150 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32062) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Sodium Potassium ATPase Alpha 3 Antibody [NBP3-35628] - Western blot analysis of various lysates using Sodium Potassium ATPase Alpha 3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35628_rabbit-sodium-potassium-atpase-alpha-3-pab-101220241233836.jpg "Western Blot: Sodium Potassium ATPase Alpha 3 Antibody [NBP3-35628] - Western blot analysis of various lysates using Sodium Potassium ATPase Alpha 3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-88330] - WB Suggested Anti-AT1A3 antibody Titration: 1 ug/mL. Sample Type: Human Large intestine Tumor](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-Western-Blot-NBP2-88330-img0001.jpg "Western Blot: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-88330] - WB Suggested Anti-AT1A3 antibody Titration: 1 ug/mL. Sample Type: Human Large intestine Tumor")
![Western Blot: Sodium Potassium ATPase Alpha 3 Overexpression Lysate (Adult Normal) [NBL1-07809] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Sodium Potassium ATPase Alpha 3.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07809-img0002.jpg "Western Blot: Sodium Potassium ATPase Alpha 3 Overexpression Lysate (Adult Normal) [NBL1-07809] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Sodium Potassium ATPase Alpha 3.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-99238] - Immunochemical staining of mouse Sodium Potassium ATPase Alpha 3 in mouse brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-3-Antibody-Immunohistochemistry-Paraffin-NBP2-99238-img0001.jpg "Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 3 Antibody [NBP2-99238] - Immunochemical staining of mouse Sodium Potassium ATPase Alpha 3 in mouse brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.")