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![Western Blot: VPS28 Antibody (OTI1A8) [NBP2-45412] - Analysis of extracts (35ug) from 9 different cell line (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/VPS28-Antibody-OTI1A8-Western-Blot-NBP2-45412-img0003.jpg "Western Blot: VPS28 Antibody (OTI1A8) [NBP2-45412] - Analysis of extracts (35ug) from 9 different cell line (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: VPS28 Antibody (OTI1A8) [NBP2-45412] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VPS28-Antibody-OTI1A8-Immunohistochemistry-NBP2-45412-img0002.jpg)
![Western Blot: VPS28 Antibody (OTI1A8) [NBP2-45412] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VPS28.](http://images.novusbio.com/fullsize/VPS28-Antibody-OTI1A8-Western-Blot-NBP2-45412-img0004.jpg)
![Immunofluorescence: VPS28 Antibody (OTI1A8) [NBP2-45412] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY VPS28.](http://images.novusbio.com/fullsize/VPS28-Antibody-OTI1A8-Immunofluorescence-NBP2-45412-img0001.jpg)
![Western Blot: VPS28 Antibody [NBP1-85976] - Analysis using Anti-VPS28 antibody NBP1-85976 (A) shows similar pattern to independent antibody NBP1-85973 (B).](http://images.novusbio.com/fullsize/VPS28-Antibody-Western-Blot-NBP1-85976-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: VPS28 Antibody [NBP1-85976] - Analysis using Anti-VPS28 antibody NBP1-85976 (A) shows similar pattern to independent antibody NBP1-85973 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: VPS28 Antibody [NBP1-85976] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VPS28-Antibody-Immunohistochemistry-Paraffin-NBP1-85976-img0006.jpg)
![Western Blot: VPS28 Antibody [NBP1-85976] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/VPS28-Antibody-Western-Blot-NBP1-85976-img0005.jpg)
![Western Blot: VPS28 Antibody [NBP1-85973] - Analysis using Anti-VPS28 antibody NBP1-85973 (A) shows similar pattern to independent antibody NBP1-85976 (B).](http://images.novusbio.com/fullsize/VPS28-Antibody-Western-Blot-NBP1-85973-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: VPS28 Antibody [NBP1-85973] - Analysis using Anti-VPS28 antibody NBP1-85973 (A) shows similar pattern to independent antibody NBP1-85976 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: VPS28 Antibody [NBP1-85973] - Staining in human duodenum and pancreas tissues using anti-VPS28 antibody. Corresponding VPS28 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VPS28-Antibody-Immunohistochemistry-Paraffin-NBP1-85973-img0008.jpg)
![Immunohistochemistry-Paraffin: VPS28 Antibody [NBP1-85973] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VPS28-Antibody-Immunohistochemistry-Paraffin-NBP1-85973-img0005.jpg)
![Immunohistochemistry-Paraffin: VPS28 Antibody [NBP1-85973] - Staining of human duodenum shows high expression.](http://images.novusbio.com/fullsize/VPS28-Antibody-Immunohistochemistry-Paraffin-NBP1-85973-img0006.jpg)
![Immunohistochemistry-Paraffin: VPS28 Antibody [NBP1-85973] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/VPS28-Antibody-Immunohistochemistry-Paraffin-NBP1-85973-img0007.jpg)
![Western Blot: VPS28 Antibody [NB100-1399] - (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/VPS28-Antibody-Western-Blot-NB100-1399-img0003.jpg "Western Blot: VPS28 Antibody [NB100-1399] - (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
![Immunohistochemistry-Paraffin: VPS28 Antibody [NB100-1399] - (10ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/VPS28-Antibody-Immunohistochemistry-Paraffin-NB100-1399-img0004.jpg)
![Western Blot: VPS28 Antibody [NBP2-20870] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/VPS28-Antibody-Western-Blot-NBP2-20870-img0001.jpg "Western Blot: VPS28 Antibody [NBP2-20870] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: VPS28 Antibody [NBP2-20870] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using VPS28 antibody (Green) at 1:200 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/VPS28-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20870-img0003.jpg)
![Immunohistochemistry-Paraffin: VPS28 Antibody [NBP2-20870] - Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/VPS28-Antibody-Immunohistochemistry-Paraffin-NBP2-20870-img0002.jpg)
![Western Blot: VPS28 Antibody (4F8M0) [NBP3-15703] - Western blot analysis of extracts of various cell lines, using VPS28 antibody (NBP3-15703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/VPS28-Antibody-4F8M0-Western-Blot-NBP3-15703-img0002.jpg "Western Blot: VPS28 Antibody (4F8M0) [NBP3-15703] - Western blot analysis of extracts of various cell lines, using VPS28 antibody (NBP3-15703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: VPS28 Antibody (4F8M0) [NBP3-15703] - Immunohistochemistry of paraffin-embedded human lung cancer using VPS28 Rabbit mAb (NBP3-15703) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/VPS28-Antibody-4F8M0-Immunohistochemistry-Paraffin-NBP3-15703-img0001.jpg)
![Western Blot: VPS28 Antibody (SR1407) [NBP3-22341] - Western blot analysis using NBP3-22341 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-22341_rabbit-vps28-mab-sr1407-5720231754856.jpg "Western Blot: VPS28 Antibody (SR1407) [NBP3-22341] - Western blot analysis using NBP3-22341 in Jurkat cell lysate.")
![Western Blot: VPS28 Protein [NBP1-30248] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/108816_1.jpg "Western Blot: VPS28 Protein [NBP1-30248] - 15% SDS-PAGE (3ug)")
![SDS-Page: VPS28 Protein [NBP1-30248] - VPS28, 25.4 kDa (221aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/VPS28-Protein-SDS-Page-NBP1-30248-img0001.jpg)
![Western Blot: VPS28 Overexpression Lysate (Adult Normal) [NBL1-17742] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VPS28.](http://images.novusbio.com/fullsize/VPS28-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17742-img0002.jpg "Western Blot: VPS28 Overexpression Lysate (Adult Normal) [NBL1-17742] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VPS28.")
![Western Blot: VPS28 Overexpression Lysate (Adult Normal) [NBL1-17743] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VPS28.](http://images.novusbio.com/fullsize/VPS28-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17743-img0002.jpg "Western Blot: VPS28 Overexpression Lysate (Adult Normal) [NBL1-17743] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for VPS28.")