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VPS28 Antibody

Images

 
Independent Antibodies: Western Blot: VPS28 Antibody [NBP1-85976] - Analysis using Anti-VPS28 antibody NBP1-85976 (A) shows similar pattern to independent antibody NBP1-85973 (B).
Immunohistochemistry-Paraffin: VPS28 Antibody [NBP1-85976] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Western Blot: VPS28 Antibody [NBP1-85976] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

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VPS28 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS28
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
VPS28 Protein (NBP1-85976PEP)
Publications
Read Publications using NBP1-85976.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VPS28 Antibody

  • ESCRT-I complex subunit VPS28
  • H-Vps28
  • MGC60323
  • vacuolar protein sorting 28 (yeast)
  • vacuolar protein sorting 28 homolog (S. cerevisiae)
  • vacuolar protein sorting-associated protein 28 homolog
  • yeast class E protein Vps28p homolog

Background

VPS28 encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS28 Antibody (NBP1-85976)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for VPS28 Antibody (NBP1-85976) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on VPS28

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Bioinformatics

Gene Symbol VPS28