Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: SAFB Antibody [NB100-2593] - Detection of Human and Mouse SAFB1 on HeLa whole cell lysate using NB100-2593. SAFB1 was also immunoprecipitated by rabbit anti-SAFB1 antibody NB100-2592.](http://images.novusbio.com/fullsize/SAFB-Antibody-Western-Blot-NB100-2593-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SAFB Antibody [NB100-2593] - Detection of Human and Mouse SAFB1 on HeLa whole cell lysate using NB100-2593. SAFB1 was also immunoprecipitated by rabbit anti-SAFB1 antibody NB100-2592.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: SAFB Antibody [NB100-2593] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SAFB1 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-NB100-2593-img0005.jpg)
![Immunohistochemistry-Paraffin: SAFB Antibody [NB100-2593] - FFPE section of human breast adenocarcinoma. Affinity purified rabbit anti-SAFB1 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-Paraffin-NB100-2593-img0001.jpg)
![Immunohistochemistry-Paraffin: SAFB Antibody [NB100-2593] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-SAFB1 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-Paraffin-NB100-2593-img0002.jpg)
![Immunohistochemistry: SAFB Antibody [NB100-2593] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SAFB1 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-NB100-2593-img0004.jpg)
![Western Blot: SAFB Antibody (5A11) [H00006294-M04] - Analysis of SAFB expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/SAFB-Antibody-5A11-Western-Blot-H00006294-M04-img0007.jpg "Western Blot: SAFB Antibody (5A11) [H00006294-M04] - Analysis of SAFB expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: SAFB Antibody (5A11) [H00006294-M04] - Analysis of monoclonal antibody to SAFB on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/SAFB-Antibody-5A11-Immunocytochemistry-Immunofluorescence-H00006294-M04-img0005.jpg)
![Immunohistochemistry-Paraffin: SAFB Antibody (5A11) [H00006294-M04] - Analysis of monoclonal antibody to SAFB on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/SAFB-Antibody-5A11-Immunohistochemistry-Paraffin-H00006294-M04-img0006.jpg)
![ELISA: SAFB Antibody (5A11) [H00006294-M04] - Detection limit for recombinant GST tagged SAFB is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SAFB-Antibody-5A11-ELISA-H00006294-M04-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: SAFB Antibody [NBP3-38485] - Immunofluorescence analysis of U-2 OS cells using SAFB Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38485_rabbit-safb-pab-1012202412061.jpg "Immunocytochemistry/ Immunofluorescence: SAFB Antibody [NBP3-38485] - Immunofluorescence analysis of U-2 OS cells using SAFB Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: SAFB Antibody [NBP3-38485] - Western blot analysis of various lysates using SAFB Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38485_rabbit-safb-pab-10122024121072.jpg)
![Immunohistochemistry: SAFB Antibody [NBP3-38485] - Immunohistochemistry analysis of paraffin-embedded Human colon using SAFB Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38485_rabbit-safb-pab-101220241154552.jpg)
![Western Blot: SAFB Antibody (JE64-94) [NBP3-32837] - Western blot analysis of SAFB on A431 cell lysates with Rabbit anti-SAFB antibody (NBP3-32837) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 103 kDa Observed band size: 150 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32837) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32837_rabbit-safb-mab-je64-94-288202412461634.jpg "Western Blot: SAFB Antibody (JE64-94) [NBP3-32837] - Western blot analysis of SAFB on A431 cell lysates with Rabbit anti-SAFB antibody (NBP3-32837) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 103 kDa Observed band size: 150 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32837) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SAFB Antibody (JE64-94) [NBP3-32837] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-SAFB antibody (NBP3-32837) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32837) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32837_rabbit-safb-mab-je64-94-298202414333691.jpg)
![Immunocytochemistry/ Immunofluorescence: SAFB Antibody (JE64-94) [NBP3-32837] - Immunocytochemistry analysis of NCI-H441 cells labeling SAFB with Rabbit anti-SAFB antibody (NBP3-32837) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SAFB antibody (NBP3-32837) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32837_rabbit-safb-mab-je64-94-2920241023421.jpg)
![Western Blot: SAFB Antibody (6F7) [NB200-332] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5% SDS-PAGE SAFB antibody dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SAFB-Antibody-6F7-Western-Blot-NB200-332-img0004.jpg "Western Blot: SAFB Antibody (6F7) [NB200-332] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5% SDS-PAGE SAFB antibody dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SAFB Antibody (6F7) [NB200-332] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SAFB-Antibody-6F7-Immunocytochemistry-Immunofluorescence-NB200-332-img0002.jpg)
![Immunohistochemistry: SAFB Antibody (6F7) [NBP3-27940] - Immunohistochemistry staining of SAFB in brain cortex tissue using SAFB monoclonal Antibody.](http://images.novusbio.com/fullsize/nbp3-27940_mouse-safb-mab-6f7-2711202420312952.jpg "Immunohistochemistry: SAFB Antibody (6F7) [NBP3-27940] - Immunohistochemistry staining of SAFB in brain cortex tissue using SAFB monoclonal Antibody.")
![Western Blot: SAFB Antibody (SR1200) [NBP3-22591] - Western blot analysis using NBP3-22591 in 293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22591_rabbit-safb-mab-sr1200-5720231531245.jpg "Western Blot: SAFB Antibody (SR1200) [NBP3-22591] - Western blot analysis using NBP3-22591 in 293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: SAFB Antibody [NBP1-83266] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83266-img0007.jpg "Immunocytochemistry/Immunofluorescence: SAFB Antibody [NBP1-83266] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: SAFB Antibody [NBP1-83266] - Staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-Paraffin-NBP1-83266-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SAFB Antibody [NBP1-83265] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83265-img0005.jpg "Immunocytochemistry/Immunofluorescence: SAFB Antibody [NBP1-83265] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: SAFB Antibody [NBP1-83265] - Staining of human cervix, uterine shows moderate nuclear and cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/SAFB-Antibody-Immunohistochemistry-Paraffin-NBP1-83265-img0004.jpg)
