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![Immunocytochemistry/Immunofluorescence: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] - SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] detects SARS-CoV-2 (COVID-19) ORF3a protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) ORF3a stained by SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (NBP3-25666) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25666_rabbit-sars-cov-2-orf3a-mab-hl1722-azide-and-bsa-free-212024941470.jpg "Immunocytochemistry/Immunofluorescence: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] - SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] detects SARS-CoV-2 (COVID-19) ORF3a protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) ORF3a stained by SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (NBP3-25666) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] - SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] detects SARS-CoV-2 (COVID-19) ORF3a protein by immunohistochemical analysis. Sample: Paraffin-embedded mock and SARS-CoV-2 (COVID-19) ORF3a transfected 293T. Green: SARS-CoV-2 (COVID-19) ORF3a stained by SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (NBP3-25666) diluted at 1:1000. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25666_rabbit-sars-cov-2-orf3a-mab-hl1722-azide-and-bsa-free-212024941488.jpg)
![Western Blot: SARS-CoV-2 ORF3a Antibody (HL1722) - Azide and BSA Free [NBP3-25666] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) ORF3a antibody [HL1722] (NBP3-25666) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25666_rabbit-sars-cov-2-orf3a-mab-hl1722-azide-and-bsa-free-2120249422642.jpg)
![Immunohistochemistry-Paraffin: SARS-CoV-2 ORF3a Antibody [NBP3-11933] - Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF3a in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 ORF3a antibody (NBP3-11933, 0.2 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF3a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF3a-Antibody-Immunohistochemistry-Paraffin-NBP3-11933-img0001.jpg "Immunohistochemistry-Paraffin: SARS-CoV-2 ORF3a Antibody [NBP3-11933] - Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF3a in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 ORF3a antibody (NBP3-11933, 0.2 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF3a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.")
![ELISA: SARS-CoV-2 ORF3a Antibody [NBP3-11933] - ELISA Validation Antibodies: SARS-CoV-2 ORF3a Antibody, NBP3-11933. A direct ELISA was performed using SARS-CoV-2 ORF3a immunogen peptide as coating antigen and the anti-SARS-CoV-2 ORF3a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF3a-Antibody-ELISA-NBP3-11933-img0002.jpg)
![Western Blot: SARS-CoV-2 ORF3a Antibody [NBP3-15985] - Western blot analysis of extracts of 293T cells, using SARS-CoV-2 ORF3a antibody (NBP3-15985) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/SARS-CoV-2-ORF3a-Antibody-Western-Blot-NBP3-15985-img0001.jpg "Western Blot: SARS-CoV-2 ORF3a Antibody [NBP3-15985] - Western blot analysis of extracts of 293T cells, using SARS-CoV-2 ORF3a antibody (NBP3-15985) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")