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![Knockout Validated: NDUFS2 Antibody (OTI1D5) [NBP2-46127] - Lysates of HAP1 (WT and NDUFS2 KO) were prepared and 40 ug of protein were processed for immunoblot with NDUFS2 antibody (OTI1D5). The Ponceau stained transfers of the blot are shown. Antibody dilution used: NBP2-46127 at 1/2000. Predicted band size: 52 kDa. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/NDUFS2-Antibody-OTI1D5-Knockout-Validated-NBP2-46127-img0013.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: NDUFS2 Antibody (OTI1D5) [NBP2-46127] - Lysates of HAP1 (WT and NDUFS2 KO) were prepared and 40 ug of protein were processed for immunoblot with NDUFS2 antibody (OTI1D5). The Ponceau stained transfers of the blot are shown. Antibody dilution used: NBP2-46127 at 1/2000. Predicted band size: 52 kDa. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Western Blot: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFS2.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Western-Blot-NBP2-46127-img0011.jpg)
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0010.jpg)
![Western Blot: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of A549 cell lysate (35ug) by using NDUFS2 monoclonal antibody.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Western-Blot-NBP2-46127-img0012.jpg)
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Adenocarcinoma of Human colon tissue using. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0001.jpg)
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0002.jpg)
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0003.jpg)
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0004.jpg)
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0005.jpg)
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0006.jpg)
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0007.jpg)
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0008.jpg)
![Immunohistochemistry: NDUFS2 Antibody (1D5) [NBP2-46127] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NDUFS2-Antibody-1D5-Immunohistochemistry-NBP2-46127-img0009.jpg)
![Immunoprecipitation: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - NDUFS2 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32639 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32639 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32639 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32639 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds](http://images.novusbio.com/fullsize/nbp3-32639_rabbit-ndufs2-mab-psh03-26-2682024158268.jpg "Immunoprecipitation: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - NDUFS2 was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32639 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32639 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32639 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32639 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 43 seconds")
![Western Blot: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - Western blot analysis of NDUFS2 on different lysates with Rabbit anti-NDUFS2 antibody (NBP3-32639) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: 293T cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: Raji cell lysate (20 ug/Lane) Lane 6: SK-Br-3 cell lysate (20 ug/Lane) Lane 7: K-562 cell lysate (20 ug/Lane) Lane 8: NIH/3T3 cell lysate (20 ug/Lane) Lane 9: RAW264.7 cell lysate (20 ug/Lane) Lane 10: PC-12 cell lysate (20 ug/Lane) Lane 11: Mouse brain tissue lysate (40 ug/Lane) Lane 12: Mouse kidney tissue lysate (40 ug/Lane) Lane 13: Rat brain tissue lysate (40 ug/Lane) Lane 14: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 53 kDa Observed band size: 45 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32639) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32639_rabbit-ndufs2-mab-psh03-26-278202416331239.jpg)
![Immunohistochemistry: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFS2 antibody (NBP3-32639) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32639) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32639_rabbit-ndufs2-mab-psh03-26-298202414262616.jpg)
![Immunocytochemistry/ Immunofluorescence: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - Immunocytochemistry analysis of A431 cells labeling NDUFS2 with Rabbit anti-NDUFS2 antibody (NBP3-32639) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NDUFS2 antibody (NBP3-32639) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32639_rabbit-ndufs2-mab-psh03-26-2920241192319.jpg)
![Flow Cytometry: NDUFS2 Antibody (PSH03-26) [NBP3-32639] - Flow cytometric analysis of A431 cells labeling NDUFS2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32639, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32639_rabbit-ndufs2-mab-psh03-26-392024884686.jpg)
![Immunohistochemistry-Paraffin: NDUFS2 Antibody [NBP2-30413] - Staining in human heart muscle and pancreas tissues using anti-NDUFS2 antibody. Corresponding NDUFS2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-Immunohistochemistry-Paraffin-NBP2-30413-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NDUFS2 Antibody [NBP2-30413] - Staining in human heart muscle and pancreas tissues using anti-NDUFS2 antibody. Corresponding NDUFS2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: NDUFS2 Antibody [NBP2-30413] - Analysis using Anti-NDUFS2 antibody NBP2-30413 (A) shows similar pattern to independent antibody NBP2-49351 (B).](http://images.novusbio.com/fullsize/NDUFS2-Antibody-Western-Blot-NBP2-30413-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: NDUFS2 Antibody [NBP2-30413] - Staining of human cell line HeLa shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30413-img0004.jpg)
![Immunohistochemistry-Paraffin: NDUFS2 Antibody [NBP2-30413] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-Immunohistochemistry-Paraffin-NBP2-30413-img0008.jpg)
![Immunohistochemistry-Paraffin: NDUFS2 Antibody [NBP2-30413] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-Immunohistochemistry-Paraffin-NBP2-30413-img0009.jpg)
![Western Blot: NDUFS2 Antibody [NBP3-03566] - Analysis of extracts of various cell lines, using NDUFS2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-Western-Blot-NBP3-03566-img0003.jpg "Western Blot: NDUFS2 Antibody [NBP3-03566] - Analysis of extracts of various cell lines, using NDUFS2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunocytochemistry/Immunofluorescence: NDUFS2 Antibody [NBP3-03566] - Analysis of L929 cells using NDUFS2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03566-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: NDUFS2 Antibody [NBP3-03566] - Analysis of extracts from normal (control) and NDUFS2 knockout (KO) 293T cells, using NDUFS2 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-Knockout-Validated-NBP3-03566-img0002.jpg)
![Western Blot: NDUFS2 Antibody (9W2N4) [NBP3-33273] - Western blot analysis of various lysates, using NDUFS2 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33273_rabbit-ndufs2-mab-9w2n4-912202415152829.jpg "Western Blot: NDUFS2 Antibody (9W2N4) [NBP3-33273] - Western blot analysis of various lysates, using NDUFS2 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Western Blot: NDUFS2 Antibody (9W2N4) [NBP3-33273] - Western blot analysis of lysates from Rat heart, using NDUFS2 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-33273_rabbit-ndufs2-mab-9w2n4-91220241418138.jpg)
![Western Blot: NDUFS2 Antibody (9W2N4) [NBP3-33273] - Western blot analysis of various lysates using NDUFS2 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33273_rabbit-ndufs2-mab-9w2n4-912202415133452.jpg)
![Western Blot: NDUFS2 Antibody (JE65-29) [NBP3-32638] - Western blot analysis of NDUFS2 on different lysates with Rabbit anti-NDUFS2 antibody (NBP3-32638) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: A431 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32638) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32638_rabbit-ndufs2-mab-je65-29-278202415242624.jpg "Western Blot: NDUFS2 Antibody (JE65-29) [NBP3-32638] - Western blot analysis of NDUFS2 on different lysates with Rabbit anti-NDUFS2 antibody (NBP3-32638) at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: A431 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32638) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NDUFS2 Antibody (JE65-29) [NBP3-32638] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFS2 antibody (NBP3-32638) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32638) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32638_rabbit-ndufs2-mab-je65-29-298202414115422.jpg)
![Western Blot: NDUFS2 Antibody [NBP1-52061] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-Western-Blot-NBP1-52061-img0002.jpg "Western Blot: NDUFS2 Antibody [NBP1-52061] - (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: NDUFS2 Antibody [NBP2-49351] - Analysis using Anti-NDUFS2 antibody NBP2-49351 (A) shows similar pattern to independent antibody NBP2-30413 (B).](http://images.novusbio.com/fullsize/NDUFS2-Antibody-Western-Blot-NBP2-49351-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NDUFS2 Antibody [NBP2-49351] - Analysis using Anti-NDUFS2 antibody NBP2-49351 (A) shows similar pattern to independent antibody NBP2-30413 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: NDUFS2 Antibody [NBP2-49351] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/NDUFS2-Antibody-Immunohistochemistry-NBP2-49351-img0001.jpg)
![Western Blot: NDUFS2 Antibody (SR1778) [NBP3-22604] - Western blot analysis using NBP3-22604 in (1) HeLa cell lysate; (2) RAW264.7 HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22604_rabbit-ndufs2-mab-sr1778-57202316494263.jpg "Western Blot: NDUFS2 Antibody (SR1778) [NBP3-22604] - Western blot analysis using NBP3-22604 in (1) HeLa cell lysate; (2) RAW264.7 HeLa cell lysate.")
![Western Blot: NDUFS2 Overexpression Lysate (Adult Normal) [NBL1-13561] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDUFS2.](http://images.novusbio.com/fullsize/NDUFS2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13561-img0002.jpg "Western Blot: NDUFS2 Overexpression Lysate (Adult Normal) [NBL1-13561] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDUFS2.")
![SDS-Page: NDUFS2 Protein [NBP2-23265]](http://images.novusbio.com/fullsize/NDUFS2-Protein-SDS-Page-NBP2-23265-img0001.jpg "SDS-Page: NDUFS2 Protein [NBP2-23265]")