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![Western Blot: IP3R1 Antibody [NBP1-21398] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-IP3R1 antibody. IP3R1 was also immunoprecipitated by rabbit anti-IP3R1 antibody NBP1-21397.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Western-Blot-NBP1-21398-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: IP3R1 Antibody [NBP1-21398] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-IP3R1 antibody. IP3R1 was also immunoprecipitated by rabbit anti-IP3R1 antibody NBP1-21397.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:100 (2ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated used ata dilution of 1:100. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-NBP1-21398-img0006.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-21398] - Section of mouse cerebellum. Antibody: Affinity purified rabbit anti- IP3R1 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-NBP1-21398-img0005.jpg)
![Western Blot: IP3R1 Antibody [NB120-5908] - G) Immunoblots of control (C) and proband (P1, P2, P3) cell lysates demonstrate equivalent levels of SERCA2b and IP3R1 Ca2+ channels using rabbit anti-SERCA2 (Novus Biologicals; NBP1-59203) and rabbit anti-IP3R1 (Novus Biologicals; NB120-5908). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27441836) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Western-Blot-NB120-5908-img0001.jpg "Western Blot: IP3R1 Antibody [NB120-5908] - G) Immunoblots of control (C) and proband (P1, P2, P3) cell lysates demonstrate equivalent levels of SERCA2b and IP3R1 Ca2+ channels using rabbit anti-SERCA2 (Novus Biologicals; NBP1-59203) and rabbit anti-IP3R1 (Novus Biologicals; NB120-5908). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27441836) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at endoplasmic reticulum by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-212024940874.jpg "Immunocytochemistry/Immunofluorescence: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at endoplasmic reticulum by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249371512.jpg)
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249351311.jpg)
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249432830.jpg)
![Western Blot: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - Whole cell extract (30 Aug) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249371526.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249425457.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-2120249362765.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (HL1541) - Azide and BSA Free [NBP3-25535] - IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (NBP3-25535) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25535_rabbit-ip3r1-mab-hl1541-azide-and-bsa-free-212024940889.jpg)
![Western Blot: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Western blot analysis of extracts of Mouse brain, using IP3R1 Rabbit mAb (NBP3-16414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Western-Blot-NBP3-16414-img0005.jpg "Western Blot: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Western blot analysis of extracts of Mouse brain, using IP3R1 Rabbit mAb (NBP3-16414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunofluorescence analysis of mouse brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-NBP3-16414-img0004.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunohistochemistry of paraffin-embedded rat brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-Paraffin-NBP3-16414-img0001.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunohistochemistry of paraffin-embedded mouse brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-Paraffin-NBP3-16414-img0002.jpg)
![Immunohistochemistry: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunofluorescence analysis of rat brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-NBP3-16414-img0003.jpg)
![Western Blot: IP3R1 Antibody [NBP1-83105] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-87 MG](http://images.novusbio.com/fullsize/IP3R1-Antibody-Western-Blot-NBP1-83105-img0004.jpg "Western Blot: IP3R1 Antibody [NBP1-83105] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-87 MG")
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83105] - Staining of human skin shows no positivity in epidermal cells as expected.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83105-img0008.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83105] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83105-img0005.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83105] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83105-img0006.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83105] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83105-img0007.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83105] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83105-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: IP3R1 Antibody [NBP2-22458] - Immunolocalization of IP3 receptor type I in rat cerebellum.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22458-img0001.jpg "Immunocytochemistry/Immunofluorescence: IP3R1 Antibody [NBP2-22458] - Immunolocalization of IP3 receptor type I in rat cerebellum.")
![Western Blot: IP3R1 Antibody [NBP3-09066] - Western blot analysis of IP3 Receptor on Hela cell lysate using anti-IP3 Receptor antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Western-Blot-NBP3-09066-img0001.jpg "Western Blot: IP3R1 Antibody [NBP3-09066] - Western blot analysis of IP3 Receptor on Hela cell lysate using anti-IP3 Receptor antibody at 1/500 dilution.")
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP3-09066] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IP3 Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP3-09066-img0003.jpg)
![Flow Cytometry: IP3R1 Antibody [NBP3-09066] - Flow cytometric analysis of SH-SY-5Y cells with IP3 Receptor antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Flow-Cytometry-NBP3-09066-img0004.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP3-09066] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IP3 Receptor antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP3-09066-img0002.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83104] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83104-img0006.jpg "Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83104] - Staining of human liver shows no positivity in hepatocytes as expected.")
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83104] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83104-img0004.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83104] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83104-img0005.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody [NBP1-83104] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/IP3R1-Antibody-Immunohistochemistry-Paraffin-NBP1-83104-img0007.jpg)
![Western Blot: IP3R1 Antibody [NBP3-37937] - Western blot analysis of various lysates using IP3R1 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): A-431.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-37937_rabbit-ip3r1-pab-912202415133447.jpg "Western Blot: IP3R1 Antibody [NBP3-37937] - Western blot analysis of various lysates using IP3R1 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): A-431.Exposure time: 10s.")
![Western Blot: IP3R1 Antibody [NBP3-37937] - Immunoprecipitation of IP3R from 500 ug extracts of SH-SY5Y cells was performed using IP3R1 Rabbit pAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using IP3R Rabbit pAb at a dilution of 1:2500.](http://images.novusbio.com/fullsize/nbp3-37937_rabbit-ip3r1-pab-9122024159564.jpg)
![Immunohistochemistry: IP3R1 Antibody [NBP3-37937] - Perform microwave antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded Rat Brain using IP3R Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37937_rabbit-ip3r1-pab-10122024140431.jpg)
![SDS-Page: Recombinant Human IP3R1 Protein [H00003708-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00003708-Q01-1.jpg "SDS-Page: Recombinant Human IP3R1 Protein [H00003708-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")