Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Western Blot: SMAC/Diablo Antibody [NB100-56311] - Analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NB100-56311-img0006.jpg "Western Blot: SMAC/Diablo Antibody [NB100-56311] - Analysis of Smac (DIABLO) in HeLa lysate using Smac antibody at 2 ug/ml.")
![Immunohistochemistry: SMAC/Diablo Antibody [NB100-56311] - Analysis of human prostate using Smac antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunohistochemistry-NB100-56311-img0007.jpg)
![Western Blot: Smac Antibody (78-1-118) [NB500-213] - HeLa whole cell extract lysates.](http://images.novusbio.com/fullsize/Smac-Antibody-78-1-118-Western-Blot-NB500-213-img0002.jpg "Western Blot: Smac Antibody (78-1-118) [NB500-213] - HeLa whole cell extract lysates.")
![Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of extracts from 293 and HeLa cell lines using this antibody at dilution of 1:1000. Image from verified customer review,](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NB100-56165-img0003.jpg "Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of extracts from 293 and HeLa cell lines using this antibody at dilution of 1:1000. Image from verified customer review,")
![Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of paraffin-embedded human lung carcinoma using this antibody at dilution of 1:25. Image from verified customer review.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunohistochemistry-Paraffin-NB100-56165-img0004.jpg)
![Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Analysis of MCF7 (A) and K562 (B) cell lysate using this antibody at 1:500.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NB100-56165-img0002.jpg)
![Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Formalin-fixed, paraffin-embedded tissue cores from a human breast cancer microarray stained for Smac/Diablo using this antibody at 1:2000. Hematoxylin-eosin counterstain. Differential Smac/DIABLO staining is seen between patient samples.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunohistochemistry-Paraffin-NB100-56165-img0001.jpg)
![Simple Western: SMAC/Diablo Antibody - Unpurified [NB100-56165] - Shows a specific band for Smac in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Simple-Western-NB100-56165-img0005.jpg)
![Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: SMAC/Diablo NBP1-76772 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NBP1-76772-img0006.jpg "Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: SMAC/Diablo NBP1-76772 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-76772] - Immunohistochemical staining of mouse spleen tissue using Smac antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunohistochemistry-Paraffin-NBP1-76772-img0002.jpg)
![Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 ug /ml.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NBP1-76772-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: SMAC/Diablo NB100-55900 (1 ug/mL), SMAC/Diablo NBP1-76772 (1 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Western-Blot-NBP1-76772-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 4 Immunofluorescence Validation of SMAC/Diablo in Mouse Spleen CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Spleen Cells labeling SMAC/Diablo with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp1-76772_rabbit-polyclonal-smac-diablo-antibody-271120242022953.jpg)
![Immunohistochemistry: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 5 Immunohistochemistry Validation of SMAC/Diablo in Mouse Spleen Tissue Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using anti-SMAC/Diablo antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76772_rabbit-polyclonal-smac-diablo-antibody-271120242004138.jpg)
![Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-84262] - Staining in human testis and skeletal muscle tissues using anti-DIABLO antibody. Corresponding DIABLO RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunohistochemistry-Paraffin-NBP1-84262-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-84262] - Staining in human testis and skeletal muscle tissues using anti-DIABLO antibody. Corresponding DIABLO RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody [NBP1-84262] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84262-img0006.jpg)
![Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-84262] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunohistochemistry-Paraffin-NBP1-84262-img0007.jpg)
![Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-84262] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-Immunohistochemistry-Paraffin-NBP1-84262-img0008.jpg)
![Western Blot: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775] - Western blot detection of SMAC/Diablo in K562, Hela cell lysates using NBP3-19775 (1:1000 diluted). Predicted band size: 27kDa. Observed band size: 21kDa.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-S08-6A3-Western-Blot-NBP3-19775-img0003.jpg "Western Blot: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775] - Western blot detection of SMAC/Diablo in K562, Hela cell lysates using NBP3-19775 (1:1000 diluted). Predicted band size: 27kDa. Observed band size: 21kDa.")
![Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775] - Immunocytochemistry of SMAC/Diablo (green) in Hela cells using NBP3-19775 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-S08-6A3-Immunocytochemistry-Immunofluorescence-NBP3-19775-img0001.jpg)
![Immunohistochemistry-Paraffin: SMAC/Diablo Antibody (S08-6A3) [NBP3-19775] - Immunohistochemistry of SMAC/Diablo in paraffin-embedded Human tonsil using NBP3-19775 at dilution 1/5](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-S08-6A3-Immunohistochemistry-Paraffin-NBP3-19775-img0002.jpg)
![Western Blot: SMAC/Diablo Antibody (006) [NBP2-89251] - Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: A431 Whole Cell Lysate Lane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:20 kDa](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-006-Western-Blot-NBP2-89251-img0001.jpg "Western Blot: SMAC/Diablo Antibody (006) [NBP2-89251] - Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: A431 Whole Cell Lysate Lane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:20 kDa")
![Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody (006) [NBP2-89251] - Staining of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Diablo monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-006-Immunocytochemistry-Immunofluorescence-NBP2-89251-img0002.jpg)
![Flow Cytometry: SMAC/Diablo Antibody (006) [NBP2-89251] - Analysis of Human Diablo expression on HeLa cells. Stained with purified anti-Human Diablo (Filled hisgram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by was blocked by preincubation of the purified antibody with 20ug recombinant human Diablo for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-006-Flow-Cytometry-NBP2-89251-img0003.jpg)
![Western Blot: SMAC/Diablo Antibody (RM271) [NBP2-61586] - HeLa and MCF-7 cells lysates using Anti-Smac/Diablo Rabbit Monoclonal Antibody at a 1:1000 dilution.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-RM271-Western-Blot-NBP2-61586-img0003.jpg "Western Blot: SMAC/Diablo Antibody (RM271) [NBP2-61586] - HeLa and MCF-7 cells lysates using Anti-Smac/Diablo Rabbit Monoclonal Antibody at a 1:1000 dilution.")
![Immunohistochemistry-Paraffin: SMAC/Diablo Antibody (RM271) [NBP2-61586] - Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using NBP2-61586.](http://images.novusbio.com/fullsize/SMAC-Diablo-Antibody-RM271-Immunohistochemistry-Paraffin-NBP2-61586-img0002.jpg)
![Western Blot: Smac Overexpression Lysate (Adult Normal) [NBP2-11272] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Smac.](http://images.novusbio.com/fullsize/Smac-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11272-img0001.jpg "Western Blot: Smac Overexpression Lysate (Adult Normal) [NBP2-11272] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Smac.")