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![Immunocytochemistry/Immunofluorescence: DCP2 Antibody [NBP2-16109] - Zebrafish blastula cells expressing GFP-Rbpms2 fusions. Wild-type GFP-Rbpms2b localizes near the nucleus (top row), is apparently associated with the centrosome/spindle in some cells. (Top row) A subset of GFP-Rbpms2b positive granules are positive for the p-body marker Dcp2. (Second row) GFP-Rbpms2bsa9329 localization to the centrosome/spindle but not granules. (Third rod) GFP-Rbpms2bae32 and (Final row) GFP-Rbpms2aae30 localization. Insets show magnified views of the highlighted cells. Images are representative slices from Z-stacks of sphere stage embryos viewed from the animal pole. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pgen.1007489) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16109-img0004.jpg "Immunocytochemistry/Immunofluorescence: DCP2 Antibody [NBP2-16109] - Zebrafish blastula cells expressing GFP-Rbpms2 fusions. Wild-type GFP-Rbpms2b localizes near the nucleus (top row), is apparently associated with the centrosome/spindle in some cells. (Top row) A subset of GFP-Rbpms2b positive granules are positive for the p-body marker Dcp2. (Second row) GFP-Rbpms2bsa9329 localization to the centrosome/spindle but not granules. (Third rod) GFP-Rbpms2bae32 and (Final row) GFP-Rbpms2aae30 localization. Insets show magnified views of the highlighted cells. Images are representative slices from Z-stacks of sphere stage embryos viewed from the animal pole. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pgen.1007489) licensed under a CC-BY license.")
![Western Blot: DCP2 Antibody [NBP2-16109] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/DCP2-Antibody-Western-Blot-NBP2-16109-img0001.jpg)
![Immunohistochemistry-Paraffin: DCP2 Antibody [NBP2-16109] - Mouse testis. DCP2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/DCP2-Antibody-Immunohistochemistry-Paraffin-NBP2-16109-img0003.jpg)
![Western Blot: DCP2 Antibody [NBP2-16109] -A. 30 μg 24hr whole zebrafish lysate/extract10 % SDS-PAGEDCP2 antibody dilution: 1:500](http://images.novusbio.com/fullsize/antibody/nbp2-16109_rabbit-polyclonal-dcp2-antibody-western-blot-151120238923..jpg)
![Western Blot: DCP2 Antibody [NBP2-16109] -Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DCP2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16109_rabbit-polyclonal-dcp2-antibody-western-blot-1511202381130..jpg)
![Immunocytochemistry/ Immunofluorescence: DCP2 Antibody [NBP2-16109] - rbpms2 mutant allele stability & localization activity in somatic cells.(A) RT-PCR to detect rbpms2a & rbpms2b maternal transcripts. Pink asterisks indicate likely nonsense mediated decay of mutant allele transcripts. (B-F) Wild-type GFP-Rbpms2a/b (B, D) & GFP-Rbpms2bsa9329 (F) localize to granules in HEK 293 cells, while GFP-Rbpms2aae30 & GFP-Rbpms2bae32 are not localized. (G-K) Zebrafish blastula cells expressing GFP-Rbpms2 fusions. (G,H) Wild-type GFP-Rbpms2b localizes near the nucleus (H), is apparently associated with the centrosome/spindle in some cells, & (G) is in granules that are not positive for the stress granule marker Tial-1. (H) A subset of GFP-Rbpms2b positive granules are positive for the p-body marker Dcp2 (open arrowheads). (I) GFP-Rbpms2bsa9329 localization to the centrosome/spindle but not granules. (J) GFP-Rbpms2bae32 & (K) GFP-Rbpms2aae30 localization. Insets show magnified views of the highlighted cells. Images are representative slices from Z-stacks of sphere stage embryos viewed from the animal pole. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29975683), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16109_rabbit-polyclonal-dcp2-antibody-31020241613632.jpg)
![Western Blot: DCP2 Antibody [NBP1-41070] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Affinity purified rabbit anti-Dcp2 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Dcp2 was also immunoprecipitated by another rabbit anti-Dcp2 antibody, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/DCP2-Antibody-Western-Blot-NBP1-41070-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DCP2 Antibody [NBP1-41070] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from 293T cells. Affinity purified rabbit anti-Dcp2 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Dcp2 was also immunoprecipitated by another rabbit anti-Dcp2 antibody, which recognizes an upstream epitope.")
![Immunocytochemistry/Immunofluorescence: DCP2 Antibody (PCRP-DCP2-1D6) [NBP3-13864] - Immunofluorescent staining of PFA-fixed human cell line MCF7. DCP2 Antibody (PCRP-DCP2-1D6) followed by goat anti-mouse IgG-CF488 (green); phalloidin (red).](http://images.novusbio.com/fullsize/DCP2-Antibody-PCRP-DCP2-1D6-Immunocytochemistry-Immunofluorescence-NBP3-13864-img0002.jpg "Immunocytochemistry/Immunofluorescence: DCP2 Antibody (PCRP-DCP2-1D6) [NBP3-13864] - Immunofluorescent staining of PFA-fixed human cell line MCF7. DCP2 Antibody (PCRP-DCP2-1D6) followed by goat anti-mouse IgG-CF488 (green); phalloidin (red).")
![Flow Cytometry: DCP2 Antibody (PCRP-DCP2-1D6) [NBP3-13864] - Flow cytometric analysis of PFA-fixed HeLa cells. DCP2 antibody (PCRP-DCP2-1D6) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).](http://images.novusbio.com/fullsize/DCP2-Antibody-PCRP-DCP2-1D6-Flow-Cytometry-NBP3-13864-img0001.jpg)
![Protein Array: DCP2 Antibody (PCRP-DCP2-1D6) [NBP3-13864] - Analysis of Protein Array containing more than 19,000 full-length human proteins using DCP2 Antibody (PCRP-DCP2-1D6).](http://images.novusbio.com/fullsize/DCP2-Antibody-PCRP-DCP2-1D6-Protein-Array-NBP3-13864-img0004.jpg)
![Western Blot: DCP2 Antibody [NBP2-81810] - Western blot analysis of DCP2 in 293 cell lysate with DCP2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/DCP2-Antibody-Western-Blot-NBP2-81810-img0001.jpg "Western Blot: DCP2 Antibody [NBP2-81810] - Western blot analysis of DCP2 in 293 cell lysate with DCP2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: DCP2 Antibody - BSA Free [NBP2-81810] - Immunofluorescence of DCP2 in human testis tissue with DCP2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81810_rabbit-polyclonal-dcp2-antibody-2711202419475878.jpg)
![Western Blot: DCP2 Antibody [NBP3-38521] - Western blot analysis of various lysates, using DCP2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38521_rabbit-dcp2-pab-912202415111811.jpg "Western Blot: DCP2 Antibody [NBP3-38521] - Western blot analysis of various lysates, using DCP2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: DCP2 Antibody [NBP2-56996] - Staining of human cell line MCF7 shows localization to nucleoplasm, cell junctions & cytoplasmic bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56996-img0001.jpg "Immunocytochemistry/Immunofluorescence: DCP2 Antibody [NBP2-56996] - Staining of human cell line MCF7 shows localization to nucleoplasm, cell junctions & cytoplasmic bodies. Antibody staining is shown in green.")
![Western Blot: DCP2 Antibody [NBP1-57441] - Human Placenta lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/DCP2-Antibody-Western-Blot-NBP1-57441-img0003.jpg "Western Blot: DCP2 Antibody [NBP1-57441] - Human Placenta lysate, concentration 0.2-1 ug/ml.")
![Western Blot: DCP2 Antibody [NBP1-57442] - Titration: 0.2-1 ug/ml Positive Control: Jurkat cell lysate.](http://images.novusbio.com/fullsize/DCP2-Antibody-Western-Blot-NBP1-57442-img0004.jpg "Western Blot: DCP2 Antibody [NBP1-57442] - Titration: 0.2-1 ug/ml Positive Control: Jurkat cell lysate.")