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![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining in human testis and liver tissues . Corresponding CIC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining in human testis and liver tissues . Corresponding CIC RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Capicua Antibody [NBP2-33420] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-CIC antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Capicua-Antibody-Western-Blot-NBP2-33420-img0013.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Capicua Antibody [NBP2-33420] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented](http://images.novusbio.com/fullsize/Capicua-Antibody-Western-Blot-NBP2-33420-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Capicua Antibody [NBP2-33420] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Staining is shown in green.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33420-img0012.jpg)
![Western Blot: Capicua Antibody [NBP2-33420] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line A-549](http://images.novusbio.com/fullsize/Capicua-Antibody-Western-Blot-NBP2-33420-img0005.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human cerebellum shows strong nuclear immunoreactivity in Purkinje cells, as well as positivity in neurons in granular and molecular layers.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0007.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular stromal cells.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0008.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human liver shows very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0009.jpg)
![Immunohistochemistry-Paraffin: Capicua Antibody [NBP2-33420] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunohistochemistry-Paraffin-NBP2-33420-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Capicua Antibody [NB110-59905] - Capicua antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunocytochemistry-Immunofluorescence-NB110-59905-img0002.jpg "Immunocytochemistry/Immunofluorescence: Capicua Antibody [NB110-59905] - Capicua antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Immunoprecipitation: Capicua Antibody [NB110-59905] - Detection of Human CIC/Capicua by WB of IPs. Whole cell lysate from HeLa cells. Affinity purified rabbit NB110-59905 used for IP at 3 mcg/mg lysate. CIC was also IPed by rabbit anti-CICa antibody.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunoprecipitation-NB110-59905-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Capicua Antibody [NBP2-56342] - Staining of human cell line A549 shows localization to nucleoplasm & vesicles.](http://images.novusbio.com/fullsize/Capicua-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56342-img0001.jpg "Immunocytochemistry/Immunofluorescence: Capicua Antibody [NBP2-56342] - Staining of human cell line A549 shows localization to nucleoplasm & vesicles.")
![Western Blot: Capicua Antibody [NBP2-92230] - Analysis of extracts of NIH/3T3, using Capicua at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/Capicua-Antibody-Western-Blot-NBP2-92230-img0001.jpg "Western Blot: Capicua Antibody [NBP2-92230] - Analysis of extracts of NIH/3T3, using Capicua at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")