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![Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Western-Blot-H00001072-M04-img0013.jpg "Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of monoclonal antibody to CFL1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Immunocytochemistry-Immunofluorescence-H00001072-M04-img0009.jpg)
![Immunohistochemistry-Paraffin: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of monoclonal antibody to CFL1 on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 1.5 ug/ml](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Immunohistochemistry-Paraffin-H00001072-M04-img0010.jpg)
![Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Western Blot analysis of CFL1 expression in PC-12(Cat # L012V1 ).](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Western-Blot-H00001072-M04-img0002.jpg)
![Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Western-Blot-H00001072-M04-img0011.jpg)
![Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-Western-Blot-H00001072-M04-img0012.jpg)
![ELISA: Cofilin Antibody (1A1) [H00001072-M04] - Detection limit for recombinant GST tagged CFL1 is approximately 10ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-1A1-ELISA-H00001072-M04-img0008.jpg)
![Western Blot: Cofilin Antibody (567) [NBP2-42828] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [567] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Western-Blot-NBP2-42828-img0011.jpg "Western Blot: Cofilin Antibody (567) [NBP2-42828] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [567] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody (567) [NBP2-42828] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: Cofilin 1 protein stained by Cofilin 1 antibody diluted at 1:300. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Immunocytochemistry-Immunofluorescence-NBP2-42828-img0005.jpg)
![Immunohistochemistry-Paraffin: Cofilin Antibody (567) [NBP2-42828] - HBL435 xenograft. Cofilin 1 antibody [567] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Immunohistochemistry-Paraffin-NBP2-42828-img0002.jpg)
![Western Blot: Cofilin Antibody (567) [NBP2-42828] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 12 % SDS-PAGE Cofilin 1 antibody [567] dilution: 1:1000](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Western-Blot-NBP2-42828-img0003.jpg)
![Western Blot: Cofilin Antibody (567) [NBP2-42828] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [567] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Western-Blot-NBP2-42828-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Cofilin Antibody (567) [NBP2-42828] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-567-Western-Blot-NBP2-42828-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Cofilin Antibody (567) [NBP2-42828] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-42828_mouse-monoclonal-cofilin-antibody-567-1610202419405739.jpg)
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Cofilin Antibody (GT217) [NBP3-13520] - Cofilin antibody [GT217] detects Cofilin protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin antibody [GT217] (NBP3-13520) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Immunohistochemistry-Paraffin-NBP3-13520-img0003.jpg)
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0004.jpg)
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Western-Blot-NBP3-13520-img0005.jpg)
![Immunoprecipitation: Cofilin Antibody (GT217) [NBP3-13520] - Immunoprecipitation of Cofilin protein from 293T whole cell extracts using 5 ug of Cofilin antibody [GT217] (NBP3-13520). Western blot analysis was performed using Cofilin antibody [GT217] (NBP3-13520) diluted at 1:500. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Cofilin-Antibody-GT217-Immunoprecipitation-NBP3-13520-img0002.jpg)
![Western Blot: Cofilin Antibody (GT217) [NBP3-13520] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin antibody [GT217] (NBP3-13520) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13520_mouse-cofilin-mab-gt217-910202420134075.jpg)
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (GT217) [NBP3-13520] - Cofilin antibody [GT217] detects Cofilin protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cofilin stained by Cofilin antibody [GT217] (NBP3-13520) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13520_mouse-cofilin-mab-gt217-91020242075996.jpg)
![Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-278202416285276.jpg "Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32207) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2982024142099.jpg)
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunocytochemistry analysis of HeLa cells labeling Cofilin with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2920241048483.jpg)
![Flow Cytometry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Flow cytometric analysis of HeLa cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32207, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-29202416372168.jpg)
![Western Blot: Cofilin Antibody [NB100-1900] - Whole extract of A-431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Anti-Cofilin antibody produced in Rabbit. The antibody was developed using 1:6000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat.Lanes:1. 1:10,000 antibody2. 1:20,000 antibody3. Negative Control](http://images.novusbio.com/fullsize/Cofilin-Antibody-Western-Blot-NB100-1900-img0002.jpg "Western Blot: Cofilin Antibody [NB100-1900] - Whole extract of A-431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Anti-Cofilin antibody produced in Rabbit. The antibody was developed using 1:6000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat.Lanes:1. 1:10,000 antibody2. 1:20,000 antibody3. Negative Control")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody [NB100-1900] - NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton X-100. Fixed cells were stained with 1:1,000 Anti-Cofilin antibody produced in Rabbit . The antibody was developed using 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat. Picture B: cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/Cofilin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1900-img0001.jpg)
![Western Blot: Cofilin [p Ser3] Antibody [NBP3-05394] - Western blot analysis of extracts of NIH/3T3 cells, using Cofilin pAb (NBP3-05394) at 1:1000 dilution or CFL1 antibody (A1704).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Western-Blot-NBP3-05394-img0003.jpg "Western Blot: Cofilin [p Ser3] Antibody [NBP3-05394] - Western blot analysis of extracts of NIH/3T3 cells, using Cofilin pAb (NBP3-05394) at 1:1000 dilution or CFL1 antibody (A1704).NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/Immunofluorescence: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunofluorescence analysis of methanol-fixed HeLa cells using Cofilin antibody (NBP3-05394).](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05394-img0002.jpg)
![Immunohistochemistry-Paraffin: Cofilin [p Ser3] Antibody [NBP3-05394] - Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Cofilin antibody (NBP3-05394).](http://images.novusbio.com/fullsize/Cofilin-[p-Ser3]-Antibody-Immunohistochemistry-Paraffin-NBP3-05394-img0001.jpg)
![Western Blot: Cofilin Antibody (S06-2C0) [NBP3-15015] - Western blot detection of Cofilin in A549, MCF-7, 293T cell lysates using Cofilin Rabbit mAb (1:1000 diluted). Predicted band size: 19kDa. Observed band size: 19kDa.](http://images.novusbio.com/fullsize/Cofilin-Antibody-S06-2C0-Western-Blot-NBP3-15015-img0001.jpg "Western Blot: Cofilin Antibody (S06-2C0) [NBP3-15015] - Western blot detection of Cofilin in A549, MCF-7, 293T cell lysates using Cofilin Rabbit mAb (1:1000 diluted). Predicted band size: 19kDa. Observed band size: 19kDa.")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody (S06-2C0) [NBP3-15015] - Immunocytochemistry of Cofilin (green) in hela using Cofilin Rabbit mAb at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/Cofilin-Antibody-S06-2C0-Immunocytochemistry-Immunofluorescence-NBP3-15015-img0002.jpg)
![Immunohistochemistry: Cofilin Antibody (S06-2C0) [NBP3-15015] - Immunohistochemical of Cofilin in Human lung cancer tissue using Cofilin antibody at dilution 1/2](http://images.novusbio.com/fullsize/Cofilin-Antibody-S06-2C0-Immunohistochemistry-NBP3-15015-img0003.jpg)
![Western Blot: Cofilin Antibody (PSH0-33) [NBP3-32204] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF-7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (24 ug/Lane) Lane 5: K-562 cell lysate (16 ug/Lane) Lane 6: SH-SY5Y cell lysate (22 ug/Lane) Lane 7: HUVEC cell lysate (30 ug/Lane) Lane 8: Jurkat cell lysate (30 ug/Lane) Lane 9: A431 cell lysate (20 ug/Lane) Lane 10: COS-1 cell lysate (30 ug/Lane) Lane 11: VERO cell lysate (30 ug/Lane) Lane 12: NIH/3T3 cell lysate (22 ug/Lane) Lane 13: PC-12 cell lysate (30 ug/Lane) Lane 14: Neuro-2a cell lysate (19 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 20 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32204) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32204_rabbit-cofilin-mab-psh0-33-27820241631651.jpg "Western Blot: Cofilin Antibody (PSH0-33) [NBP3-32204] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF-7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (24 ug/Lane) Lane 5: K-562 cell lysate (16 ug/Lane) Lane 6: SH-SY5Y cell lysate (22 ug/Lane) Lane 7: HUVEC cell lysate (30 ug/Lane) Lane 8: Jurkat cell lysate (30 ug/Lane) Lane 9: A431 cell lysate (20 ug/Lane) Lane 10: COS-1 cell lysate (30 ug/Lane) Lane 11: VERO cell lysate (30 ug/Lane) Lane 12: NIH/3T3 cell lysate (22 ug/Lane) Lane 13: PC-12 cell lysate (30 ug/Lane) Lane 14: Neuro-2a cell lysate (19 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 20 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32204) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH0-33) [NBP3-32204] - Immunocytochemistry analysis of NIH/3T3 cells labeling Cofilin with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (NBP3-32204) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32204_rabbit-cofilin-mab-psh0-33-29202410334062.jpg)
![Flow Cytometry: Cofilin Antibody (PSH0-33) [NBP3-32204] - Flow cytometric analysis of Hela cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32204, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32204_rabbit-cofilin-mab-psh0-33-29202416372129.jpg)
![Western Blot: Cofilin Antibody [NB100-81866] - Analysis of extracts from MDA cells using cofilin (Ab-3)](http://images.novusbio.com/fullsize/Cofilin-Antibody-Western-Blot-NB100-81866-img0001.jpg "Western Blot: Cofilin Antibody [NB100-81866] - Analysis of extracts from MDA cells using cofilin (Ab-3)")
![Immunohistochemistry-Paraffin: Cofilin Antibody [NB100-81866] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81866 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/Cofilin-Antibody-Immunohistochemistry-NB100-81866-img0002.jpg)
![Western Blot: Cofilin Antibody (1C1) [NBP1-21044] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: K562 cell lysateLane 3.: MCF7 cell lysateLane 4.: LnCap cell lysateLane 5.: PC3 cell lysate](http://images.novusbio.com/fullsize/Cofilin-Antibody-1C1-Western-Blot-NBP1-21044-img0007.jpg "Western Blot: Cofilin Antibody (1C1) [NBP1-21044] - The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cofilin 1(1:1000) antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: K562 cell lysateLane 3.: MCF7 cell lysateLane 4.: LnCap cell lysateLane 5.: PC3 cell lysate")
![Immunocytochemistry/Immunofluorescence: Cofilin Antibody (1C1) [NBP1-21044] - Analysis of Cofilin1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Cofilin1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Cofilin-Antibody-1C1-Immunocytochemistry-Immunofluorescence-NBP1-21044-img0006.jpg)
![Western Blot: Cofilin [p Ser3] Antibody [NBP3-23231] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).](http://images.novusbio.com/fullsize/nbp3-23231_rabbit-cofilin-p-ser3-pab-199202312403712.jpg "Western Blot: Cofilin [p Ser3] Antibody [NBP3-23231] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).")
![Western Blot: Cofilin Antibody [NBP3-23230] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).](http://images.novusbio.com/fullsize/nbp3-23230_rabbit-cofilin-pab-19920231759441.jpg "Western Blot: Cofilin Antibody [NBP3-23230] - Western blot of Cofilin in jurkat cells. The blots were untreated (lanes 1 & 4) or treated (lanes 2, 3, 5 & 6) with lambda phosphatase. In lanes 3 & 6, the phosphatase was inhibited with phospho-Cofilin (Ser-3) peptide. The blots were probed with anti-Cofilin (Ser-3) phospho-specific (lanes 1-3) or anti-Cofilin (N-terminus) (lanes 4-6).")