Hu,
Mu,
RtSpecies Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IP, WB, ChIP, KD
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IP, WB, ChIP, KD
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: DEC1 Antibody - BSA Free [NB100-1800] - Western Blot Image of anti-DEC1. Whole cell protein from A431 (lane 1) and PC3 (lane 2) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-DEC1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/DEC1-Antibody---BSA-Free-Western-Blot-NB100-1800-img0011.jpg "Western Blot: DEC1 Antibody - BSA Free [NB100-1800] - Western Blot Image of anti-DEC1. Whole cell protein from A431 (lane 1) and PC3 (lane 2) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-DEC1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: DEC1 Antibody - BSA Free [NB100-1800] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-DEC1 (NB100-1800) at a 1:200 dilution overnight at 4C and detected with and anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/DEC1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-1800-img0009.jpg)
![Immunohistochemistry: DEC1 Antibody - BSA Free [NB100-1800] - Immunohistochemical detection of DEC1 in myocardial and stromal cells. Representative images of one WT heart treated with TAC and sham at four weeks. The black square shows representative large images, magnification 400x. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/20/19/4967) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DEC1-Antibody---BSA-Free-Immunohistochemistry-NB100-1800-img0017.jpg)
![Flow Cytometry: DEC1 Antibody - BSA Free [NB100-1800] - An intracellular stain was performed on A431 cells with DEC1 Antibody NB100-1800AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/DEC1-Antibody---BSA-Free-Flow-Cytometry-NB100-1800-img0016.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: DEC1 Antibody - BSA Free [NB100-1800] - DEC1 expression decreases in HeLa cells under apoptosis. Western blotting images of cleaved-poly (ADP-ribose) polymerase (PARP), cleaved-caspase 3, Bcl-2, DEC1, DEC2, SOX2, c-MYC and actin treated with 10, 20, and 50 uM cisplatin or mock in HeLa and SiHa cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33089188/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DEC1 Antibody - BSA Free-Western Blot-NB100-1800-img0018.jpg)
![Western Blot: DEC1 Antibody - BSA Free [NB100-1800] - Western blot analysis of DEC1 in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/DEC1-Antibody---BSA-Free-Western-Blot-NB100-1800-img0001.jpg)
![Immunohistochemistry-Paraffin: DEC1 Antibody - BSA Free [NB100-1800] - P. gingivalis-challenged rat maxilla using DEC1 antibody at 1:100. Heat mediated antigen retrieval (sodium citrate buffer pH 6.0) was performed. Counter-stained with hematoxylin. Image from verified customer review.](http://images.novusbio.com/fullsize/DEC1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-1800-img0012.jpg)
![Immunoprecipitation: DEC1 Antibody - BSA Free [NB100-1800] - Samples: Whole cell lysate from HeLa (50 ug for WB) and 293T (1 mg/IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-DEC1 antibody BL2928 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and for IP at 3 ug/mg lysate. DEC1 was also immunoprecipitated using rabbit anti-DEC1 antibodies BL2926 and BL2927, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/DEC1-Antibody---BSA-Free-Immunoprecipitation-NB100-1800-img0007.jpg)
![Simple Western: DEC1 Antibody - BSA Free [NB100-1800] - Simple Western lane view shows a specific band for DEC1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/DEC1-Antibody---BSA-Free-Simple-Western-NB100-1800-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Immunoblotting: DEC1 Antibody - BSA Free [NB100-1800] - (B) HSC-2 cells were used to perform knockdown assays for three primary antibodies: HIF1A, DEC1, and DEC2 9. Real-time RT-PCR evaluated expression levels of MSH2, MBD4, MRE11A, BRCA1, and RAD51. Value displayed above the bar represents the ratio of expression under hypoxic conditions compared with that under normoxia. Citation: Nakamura H, Bono H, Hiyama K, Kawamoto T, Kato Y, Nakanishi T, et al. (2018) Differentiated embryo chondrocyte plays a crucial role in DNA damage response via transcriptional regulation under hypoxic conditions. PLoS ONE 13(2): e0192136. //doi.org/10.1371/journal.pone.0192136](http://images.novusbio.com/fullsize/DEC1-Antibody---BSA-Free-Immunoblotting-NB100-1800-img0013.jpg)
![Immunoblotting: DEC1 Antibody - BSA Free [NB100-1800] - DNA-DRR genes promoter activities decreased with DEC. (B) The DEC1 or DEC2 expression plasmids (B), each reporter, and DEC1 or delta basic DEC1 expression plasmids (C) were co-transfected into HepG2. Under normoxic conditions they were then incubated for 36 hours. A dual-luciferase assay system determined luciferase activities. Citation: Nakamura H, Bono H, Hiyama K, Kawamoto T, Kato Y, Nakanishi T, et al. (2018) Differentiated embryo chondrocyte plays a crucial role in DNA damage response via transcriptional regulation under hypoxic conditions. PLoS ONE 13(2): e0192136. //doi.org/10.1371/journal.pone.0192136](http://images.novusbio.com/fullsize/DEC1-Antibody---BSA-Free-Immunoblotting-NB100-1800-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Immunoblotting: DEC1 Antibody - BSA Free [NB100-1800] - Knockdown assays in HSC-2 cells for HIF1A, DEC1, and DEC2. After transfecting and culturing non-specific siRNA or targeted siRNA into HSC-2, culturing them under normoxic and hypoxic conditions (24 hours), RT-PCR evaluated expression levels of the three primary antibodies. Citation: Nakamura H, Bono H, Hiyama K, Kawamoto T, Kato Y, Nakanishi T, et al. (2018) Differentiated embryo chondrocyte plays a crucial role in DNA damage response via transcriptional regulation under hypoxic conditions. PLoS ONE 13(2): e0192136. //doi.org/10.1371/journal.pone.0192136](http://images.novusbio.com/fullsize/DEC1-Antibody---BSA-Free-Immunoblotting-NB100-1800-img0015.jpg)
![Immunohistochemistry: DEC1 Antibody - BSA Free [NB100-1800] - Dec1 expression is increased in the myocardial cells of cardiac hypertrophy & myocardial infarction. Immunohistochemical detection of Dec1 in human cardiac diseases. Representative image of Dec1 immunoreactivities from case 1 to case 8. Case 1 (control) had no significant findings (NS). Cases 2 to 6 are cardiac hypertrophy (CH). Case 7 is acute myocardial infarction (AMI), & case 8 is an old myocardial infarction (OMI). Magnification 400×. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31597354), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1800_rabbit-polyclonal-dec1-antibody-31020241539591.jpg)
![Western Blot: DEC1 Antibody - BSA Free [NB100-1800] - DEC1 has anti-apoptotic effects in HeLa cells under apoptosis. (A) Cell viability of HeLa, SiHa & Caski cells with (+) or without (−) cisplatin & empty vector, DEC1 or DEC2 plasmid treatment was determined. The absorbance (OD480/OD650) at 0 & 24 h is shown. The value of cell viability of control 0 h regarded as 10. Data are expressed as mean values ± SE (bars) of three independent samples. * p < 0.05, as determined using t-test. (B) Western blotting images of DEC1, DEC2, cleaved-PARP, cleaved-caspase 3, Bcl-2, beta -catenin, SOX2, c-MYC & actin treated with 50 μM cisplatin & empty vector, DEC1 or DEC2 plasmid in HeLa cells. Western blot analysis was repeated three times & similar results were obtained. (C) SOX2 & c-MYC mRNA expressions in HeLa & SiHa cells with cisplatin & empty vector, DEC1 or DEC2 plasmid treatment. Data are expressed as mean values ± SE (bars) of three independent samples. * p < 0.01, as determined using t-test. qPCR was repeated three times & similar results were obtained. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33089188), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1800_rabbit-polyclonal-dec1-antibody-310202415535182.jpg)
![Immunohistochemistry: DEC1 Antibody - BSA Free [NB100-1800] - Immunoreactivities of DEC1, DEC2, SOX2, c-MYC & vimentin in cervical cancer tissues. Representative images for the immunoreactivities of DEC1, DEC2, SOX2, c-MYC & vimentin in cervical cancer tissues. Each panel shows ×200 magnification. DEC1 immunoreactivities in (A) non-cancerous cells, (B) shallow cancer cells, & (C) deep cancer cells of case 14. DEC1 immunoreactivity in (D) SCC of case 1, & in (E) AC of case 5, respectively. DEC2 immunoreactivities in (F) non-cancerous cells, (G) & cancer cells of case 15. SOX2 immunoreactivities in (H) non-cancerous cells, (I) shallow cancer cells, & (J) deep cancer cells of case 14. c-MYC immunoreactivities in (K) non-cancerous cells, (L) & cancer cells of case 15. Vimentin immunoreactivities in (M) non-cancerous vascular cells, (N) & cancerous vascular cells of case 14. Black arrows in E-G show nuclear staining. The white arrow in E indicates non-cancerous cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33089188), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1800_rabbit-polyclonal-dec1-antibody-31020241555240.jpg)
![Western Blot: DEC1 Antibody - BSA Free [NB100-1800] - DEC1 expression decreases in HeLa cells under apoptosis. (A) Endogenous DEC1 protein expressions in HeLa & SiHa cells. Western blotting images of beta -catenin, DEC1, DEC2, SOX2, c-MYC & actin in HeLa & SiHa cells. The western blot analysis was repeated three times & similar results were obtained. (B) Cell viability of HeLa & SiHa cells with (+) or without (−) cisplatin treatment was determined. The absorbance (OD480/OD650) at 0 & 24 h is shown. The value of cell viability of control 0 h regarded as 10, which means each basal value without treatment. Data are expressed as mean values ± SE (bars) of three independent samples. * p <0.01, as determined using Dunnett’s test. Cis: Cisplatin treatment. (C) Western blotting images of cleaved-poly (ADP-ribose) polymerase (PARP), cleaved-caspase 3, Bcl-2, DEC1, DEC2, SOX2, c-MYC & actin treated with 10, 20, & 50 μM cisplatin or mock in HeLa & SiHa cells. The western blot analysis was repeated three times & similar results were obtained (D) DEC1, DEC2 & SOX2 mRNA expressions in HeLa & SiHa cells with (+) or without (−) cisplatin treatment. Data are expressed as mean values ± SE (bars) of three independent samples. * p < 0.01, as determined using t-test. qPCR was repeated three times & similar results were obtained. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33089188), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1800_rabbit-polyclonal-dec1-antibody-310202415541956.jpg)
![Immunohistochemistry: DEC1 Antibody - BSA Free [NB100-1800] - Immunoreactivities of DEC1, DEC2, SOX2, c-MYC & vimentin in cervical cancer tissues. Representative images for the immunoreactivities of DEC1, DEC2, SOX2, c-MYC & vimentin in cervical cancer tissues. Each panel shows ×200 magnification. DEC1 immunoreactivities in (A) non-cancerous cells, (B) shallow cancer cells, & (C) deep cancer cells of case 14. DEC1 immunoreactivity in (D) SCC of case 1, & in (E) AC of case 5, respectively. DEC2 immunoreactivities in (F) non-cancerous cells, (G) & cancer cells of case 15. SOX2 immunoreactivities in (H) non-cancerous cells, (I) shallow cancer cells, & (J) deep cancer cells of case 14. c-MYC immunoreactivities in (K) non-cancerous cells, (L) & cancer cells of case 15. Vimentin immunoreactivities in (M) non-cancerous vascular cells, (N) & cancerous vascular cells of case 14. Black arrows in E-G show nuclear staining. The white arrow in E indicates non-cancerous cells. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33089188), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1800_rabbit-polyclonal-dec1-antibody-31020241612633.jpg)
![Western Blot: DEC1 Antibody [NBP2-47488] - Analysis in control (vector only transfected HEK293T lysate) and BHLHE40 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/DEC1-Antibody-Western-Blot-NBP2-47488-img0006.jpg "Western Blot: DEC1 Antibody [NBP2-47488] - Analysis in control (vector only transfected HEK293T lysate) and BHLHE40 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: DEC1 Antibody [NBP2-47488] - Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies.](http://images.novusbio.com/fullsize/DEC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47488-img0004.jpg)
![Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488] - Staining of human urinary bladder shows moderate to strong nuclear positivity in urothelial cells.](http://images.novusbio.com/fullsize/DEC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47488-img0011.jpg)
![Western Blot: DEC1 Antibody [NBP2-47488] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/DEC1-Antibody-Western-Blot-NBP2-47488-img0005.jpg)
![Immunohistochemistry: DEC1 Antibody [NBP2-47488] - Staining of nasopharynx tissue. Shows strong nuclear positivity in respiratory epithelial cells.](http://images.novusbio.com/fullsize/DEC1-Antibody-Immunohistochemistry-NBP2-47488-img0001.jpg)
![Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488] - Staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/DEC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47488-img0007.jpg)
![Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488] - Staining of human lymph node shows moderate nuclear positivity in a small subset of cells in non-germinal center.](http://images.novusbio.com/fullsize/DEC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47488-img0008.jpg)
![Immunohistochemistry: DEC1 Antibody [NBP2-47488] - Staining of human pancreas shows low positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/DEC1-Antibody-Immunohistochemistry-NBP2-47488-img0009.jpg)
![Immunohistochemistry-Paraffin: DEC1 Antibody [NBP2-47488] - Staining of human prostate shows moderate to strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/DEC1-Antibody-Immunohistochemistry-Paraffin-NBP2-47488-img0010.jpg)
![Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1. Analysis of BHLHB2 expression in NIH/3T3.](http://images.novusbio.com/fullsize/DEC1-Antibody-5B1-Western-Blot-H00008553-M01-img0009.jpg "Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1. Analysis of BHLHB2 expression in NIH/3T3.")
![Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - Western Blot analysis of BHLHB2 expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/DEC1-Antibody-5B1-Western-Blot-H00008553-M01-img0002.jpg)
![Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1 Analysis of BHLHB2 expression in HepG2.](http://images.novusbio.com/fullsize/DEC1-Antibody-5B1-Western-Blot-H00008553-M01-img0007.jpg)
![Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1. Analysis of BHLHB2 expression in Raw 264.7.](http://images.novusbio.com/fullsize/DEC1-Antibody-5B1-Western-Blot-H00008553-M01-img0008.jpg)
![Western Blot: DEC1 Antibody (5B1) [H00008553-M01] - BHLHB2 monoclonal antibody (M01), clone 5B1. Analysis of BHLHE40 expression in K-562.](http://images.novusbio.com/fullsize/DEC1-Antibody-5B1-Western-Blot-H00008553-M01-img0010.jpg)
![ELISA: DEC1 Antibody (5B1) [H00008553-M01] - Detection limit for recombinant GST tagged BHLHB2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DEC1-Antibody-5B1-ELISA-H00008553-M01-img0011.jpg)
![Western Blot: DEC1 Antibody (PSH01-90) [NBP3-32946] - Western blot analysis of 45627 on different lysates with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 46/50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32946) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32946_rabbit-dec1-mab-psh01-90-288202412442966.jpg "Western Blot: DEC1 Antibody (PSH01-90) [NBP3-32946] - Western blot analysis of 45627 on different lysates with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 46/50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32946) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DEC1 Antibody (PSH01-90) [NBP3-32946] - Immunohistochemical analysis of paraffin-embedded human bladder tissue with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32946) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32946_rabbit-dec1-mab-psh01-90-298202413585575.jpg)
![Immunocytochemistry/ Immunofluorescence: DEC1 Antibody (PSH01-90) [NBP3-32946] - Immunocytochemistry analysis of MCF7 cells labeling DEC1 with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DEC1 antibody (NBP3-32946) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32946_rabbit-dec1-mab-psh01-90-29202410234280.jpg)
![Western Blot: DEC1 Antibody [NBP2-92900] - Analysis of extracts of various cell lines, using 44166 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/DEC1-Antibody-Western-Blot-NBP2-92900-img0004.jpg "Western Blot: DEC1 Antibody [NBP2-92900] - Analysis of extracts of various cell lines, using 44166 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunohistochemistry;DEC1 Antibody[NBP3-28997]Detection of human DEC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DEC1 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-28998_rabbit-dec1-pab-782024936131.jpg "Immunohistochemistry;DEC1 Antibody[NBP3-28997]Detection of human DEC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DEC1 used at a dilution of 1:1,000 . Detection: DAB")
![Western Blot: DEC1 Overexpression Lysate (Adult Normal) [NBL1-07974] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DEC1.](http://images.novusbio.com/fullsize/DEC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07974-img0002.jpg "Western Blot: DEC1 Overexpression Lysate (Adult Normal) [NBL1-07974] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DEC1.")
![Western Blot: DEC1 Overexpression Lysate (Adult Normal) [NBL1-07974] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for BHLHE40](http://images.novusbio.com/fullsize/DEC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07974-img0001.jpg)
![Western Blot: DEC1 Antibody (PSH01-91) [NBP3-32947] - Western blot analysis of 45627 on different lysates with Rabbit anti-45627 antibody (NBP3-32947) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32947) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32947_rabbit-dec1-mab-psh01-91-288202412442916.jpg "Western Blot: DEC1 Antibody (PSH01-91) [NBP3-32947] - Western blot analysis of 45627 on different lysates with Rabbit anti-45627 antibody (NBP3-32947) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: 293T cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32947) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")