| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79930 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79931 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14283 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14284 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03442-20ul | |||
![Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody [NBP1-89146] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89146-img0006.jpg "Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody [NBP1-89146] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0016.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining in human adrenal gland and cerebral cortex tissues using anti-AKR1B1 antibody. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Analysis in human adrenal gland and liver tissues. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0012.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human adrenal gland shows moderate to strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0013.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP1-89146-img0014.jpg)
![Immunohistochemistry: AKR1B1 Antibody [NBP1-89146] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-NBP1-89146-img0015.jpg)
![Western Blot: AKR1B1 Antibody (CPTC-AKR1B1-3) [NBP2-79796] - Western Blot Analysis of Human Kidney tissue, Human HepG2, HEK293, HeLa and Jurkat cell lysate using AKR1B1 Antibody (CPTC-AKR1B1-3).](http://images.novusbio.com/fullsize/AKR1B1-Antibody-CPTC-AKR1B1-3-Western-Blot-NBP2-79796-img0004.jpg "Western Blot: AKR1B1 Antibody (CPTC-AKR1B1-3) [NBP2-79796] - Western Blot Analysis of Human Kidney tissue, Human HepG2, HEK293, HeLa and Jurkat cell lysate using AKR1B1 Antibody (CPTC-AKR1B1-3).")
![Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody (CPTC-AKR1B1-3) [NBP2-79796] - Immunofluorescence Analysis of A549 cells labeling AKR1B1 with AKR1B1 Antibody (CPTC-AKR1B1-3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red)](http://images.novusbio.com/fullsize/AKR1B1-Antibody-CPTC-AKR1B1-3-Immunocytochemistry-Immunofluorescence-NBP2-79796-img0001.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody (CPTC-AKR1B1-3) [NBP2-79796] - Formalin-fixed, paraffin-embedded human Prostate Tumor stained with AKR1B1 Antibody (CPTC-AKR1B1-3).](http://images.novusbio.com/fullsize/AKR1B1-Antibody-CPTC-AKR1B1-3-Immunohistochemistry-Paraffin-NBP2-79796-img0002.jpg)
![Protein Array: AKR1B1 Antibody (CPTC-AKR1B1-3) [NBP2-79796] - Analysis of Protein Array containing more than 19,000 full-length human proteins using AKR1B1 Antibody (CPTC-AKR1B1-3). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-CPTC-AKR1B1-3-Protein-Array-NBP2-79796-img0003.jpg)
![Western Blot: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797] - Western Blot Analysis of HeLa cell lysate usingAKR1B1 Antibody (CPTC-AKR1B1-2).](http://images.novusbio.com/fullsize/AKR1B1-Antibody-CPTC-AKR1B1-2-Western-Blot-NBP2-79797-img0004.jpg "Western Blot: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797] - Western Blot Analysis of HeLa cell lysate usingAKR1B1 Antibody (CPTC-AKR1B1-2).")
![Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797] - Immunofluorescence Analysis of A549 cells labeling AKR1B1 with AKR1B1 Antibody (CPTC-AKR1B1-2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Blue)](http://images.novusbio.com/fullsize/AKR1B1-Antibody-CPTC-AKR1B1-2-Immunocytochemistry-Immunofluorescence-NBP2-79797-img0001.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797] - Formalin-fixed, paraffin-embedded human prostate stained with AKR1B1 Antibody (CPTC-AKR1B1-2).](http://images.novusbio.com/fullsize/AKR1B1-Antibody-CPTC-AKR1B1-2-Immunohistochemistry-Paraffin-NBP2-79797-img0002.jpg)
![Protein Array: AKR1B1 Antibody (CPTC-AKR1B1-2) [NBP2-79797] - Analysis of Protein Array containing more than 19,000 full-length human proteins using AKR1B1 Antibody (CPTC-AKR1B1-2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5](http://images.novusbio.com/fullsize/AKR1B1-Antibody-CPTC-AKR1B1-2-Protein-Array-NBP2-79797-img0003.jpg)
![Western Blot: AKR1B1 Antibody [NBP2-56910] - Western blot analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Western-Blot-NBP2-56910-img0002.jpg "Western Blot: AKR1B1 Antibody [NBP2-56910] - Western blot analysis in human cell line RT-4.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP2-56910] - Staining in human adrenal gland and cerebral cortex tissues using anti-AKR1B1 antibody. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56910-img0006.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP2-56910] - Staining of human adrenal gland shows high expression.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56910-img0003.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP2-56910] - Staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56910-img0004.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP2-56910] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-Paraffin-NBP2-56910-img0005.jpg)
![Western Blot: AKR1B1 Antibody [NBP1-53144] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: MCF7 cell lysate](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Western-Blot-NBP1-53144-img0004.jpg "Western Blot: AKR1B1 Antibody [NBP1-53144] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:312500 Positive Control: MCF7 cell lysate")
![Immunohistochemistry: AKR1B1 Antibody [NBP1-53144] - Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: N/A Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunohistochemistry-NBP1-53144-img0003.jpg)
![Western Blot: AKR1B1 Antibody [NBP1-53144] - MCF-7 whole cell lysates, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Western-Blot-NBP1-53144-img0002.jpg)
![Western Blot: AKR1B1 Antibody (109) [NBP2-89882] - Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:37 kDa](http://images.novusbio.com/fullsize/AKR1B1-Antibody-109-Western-Blot-NBP2-89882-img0001.jpg "Western Blot: AKR1B1 Antibody (109) [NBP2-89882] - Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell LysateLane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:35 kDa Observed band size:37 kDa")
![Immunocytochemistry/Immunofluorescence: AKR1B1 Antibody (109) [NBP2-89882] - Staining of Human AKR1B1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human AKR1B1 monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-109-Immunocytochemistry-Immunofluorescence-NBP2-89882-img0003.jpg)
![Immunoprecipitation: AKR1B1 Antibody (109) [NBP2-89882] - Lane A:0.5 mg Jurkat Whole Cell Lysate 0.5 uL anti-AKR1B1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-AKR1B1 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size: 35 kDa](http://images.novusbio.com/fullsize/AKR1B1-Antibody-109-Immunoprecipitation-NBP2-89882-img0002.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody (rAKR1B1/7295) [NBP3-13895] - Formalin-fixed, paraffin-embedded human adrenal gland stained with AKR1B1 antibody (rAKR1B1/7295). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-rAKR1B1-7295-Immunohistochemistry-Paraffin-NBP3-13895-img0001.jpg "Immunohistochemistry-Paraffin: AKR1B1 Antibody (rAKR1B1/7295) [NBP3-13895] - Formalin-fixed, paraffin-embedded human adrenal gland stained with AKR1B1 antibody (rAKR1B1/7295). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: AKR1B1 Antibody (AKR1B1/7009R) [NBP3-13896] - Formalin-fixed, paraffin-embedded human kidney stained with AKR1B1 antibody (AKR1B1/7009R).](http://images.novusbio.com/fullsize/AKR1B1-Antibody-AKR1B1-7009R-Immunohistochemistry-Paraffin-NBP3-13896-img0001.jpg "Immunohistochemistry-Paraffin: AKR1B1 Antibody (AKR1B1/7009R) [NBP3-13896] - Formalin-fixed, paraffin-embedded human kidney stained with AKR1B1 antibody (AKR1B1/7009R).")
![Immunohistochemistry-Paraffin: AKR1B1 Antibody (AKR1B1/7009R) [NBP3-13896] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Staining using AKR1B1 antibody (AKR1B1/7009R) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-AKR1B1-7009R-Immunohistochemistry-Paraffin-NBP3-13896-img0002.jpg)
![Immunohistochemistry-Paraffin: AKR1B1 Antibody (AKR1B1/7010R) [NBP3-13897] - Formalin-fixed, paraffin-embedded human kidney stained with AKR1B1 antibody (AKR1B1/7010R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-AKR1B1-7010R-Immunohistochemistry-Paraffin-NBP3-13897-img0001.jpg "Immunohistochemistry-Paraffin: AKR1B1 Antibody (AKR1B1/7010R) [NBP3-13897] - Formalin-fixed, paraffin-embedded human kidney stained with AKR1B1 antibody (AKR1B1/7010R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Immunohistochemistry-Paraffin: AKR1B1 Antibody (AKR1B1/7010R) [NBP3-13897] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Staining using AKR1B1 antibody (AKR1B1/7010R) at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-AKR1B1-7010R-Immunohistochemistry-Paraffin-NBP3-13897-img0002.jpg)
![Western Blot: AKR1B1 Antibody [NBP2-97585] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: 293T Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Western-Blot-NBP2-97585-img0001.jpg "Western Blot: AKR1B1 Antibody [NBP2-97585] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: 293T Whole Cell LysateLane D: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa")
![Immunoprecipitation: AKR1B1 Antibody [NBP2-97585] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-AKR1B1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AKR1B1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size :34 kDa](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunoprecipitation-NBP2-97585-img0002.jpg)
![Western Blot: AKR1B1 Antibody [NBP2-99771] - Lane A: Hela Whole Cell LysateLane B: MCF-7 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Western-Blot-NBP2-99771-img0002.jpg "Western Blot: AKR1B1 Antibody [NBP2-99771] - Lane A: Hela Whole Cell LysateLane B: MCF-7 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: AKR1B1 Antibody [NBP2-99771] - Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg A431 Whole Cell Lysate2 uL anti-AKR1B1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AKR1B1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Immunoprecipitation-NBP2-99771-img0001.jpg)
![Knockout Validated: AKR1B1 Antibody [NBP3-03442] - Analysis of extracts from normal (control) and AKR1B1 knockout (KO) HeLa cells, using AKR1B1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in tbst.](http://images.novusbio.com/fullsize/AKR1B1-Antibody-Knockout-Validated-NBP3-03442-img0001.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: AKR1B1 Antibody [NBP3-03442] - Analysis of extracts from normal (control) and AKR1B1 knockout (KO) HeLa cells, using AKR1B1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in tbst.")