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![Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Western blot analysis of NALP12 in human brain tissue lysate with NALP12 antibody at 1 u/mL.](http://images.novusbio.com/fullsize/nbp1-76293_rabbit-polyclonal-nalp12-antibody-2711202420414566.gif "Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Western blot analysis of NALP12 in human brain tissue lysate with NALP12 antibody at 1 u/mL.")
![Immunohistochemistry-Paraffin: NALP12 Antibody [NBP1-76293] - Human brain tissue. Dilution: 5 ug/mL](http://images.novusbio.com/fullsize/NALP12-Antibody-Immunohistochemistry-Paraffin-NBP1-76293-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: NALP12 Antibody - BSA Free [NBP1-76293] - Immunofluorescence of NALP12 in human brain tissue with NALP12 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76293_rabbit-polyclonal-nalp12-antibody-2711202420115531.jpg)
![Western Blot: NALP12 Antibody [NBP1-76293] - B6 mice (n = 6) were infected ocularly with 2.5 x 105 pfu/eye of HSV-1 laboratory strains McKrae, 17, F, RE or KOS or with clinical isolates KOS79 and KOS63 (mock-infected mice (n = 6) used as controls). 48 hrs later, animals were euthanized & corneas were excised & homogenized in lysis buffer, and WB was performed for NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 (beta-actin used as a control). Three WB performed using different corneal lysates harvested from three sets of corneas from 2 mice (4 corneas/lysate). Graphs (mean plus SD) show fold change in expression of NLRP3, NLRP6, NLRP12, IFI16/p204, and AIM2 at 48 hrs post-infection compared to controls, and normalized to beta-actin. Representative densitometric analysis is shown compared to control. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/articles/10.3389/fimmu.2019.01631/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NALP12-Antibody-Western-Blot-NBP1-76293-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NALP12 Antibody [NBP1-76293] - Expression level of NLRP3, NLRP6, NLRP12, IFI16 and AIM2 inflammasomes in human primary corneal epithelial cells infected with virulent and less-virulent strains of HSV-1. Human primary corneal epithelial cells (hTCEpi cell line) were infected in vitro with clinical isolates KOS79 and KOS63 at MOI of 1. Two, 8 and 24 h post-infection, hTCEpi cells were harvested and immunoblots of whole cell lysates were performed for expression of NLRP3, NLRP6, NLRP12, IFI16, and AIM2 inflammasomes. Corresponding b-actin was used as a control. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2019.01631/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NALP12-Antibody-Western-Blot-NBP1-76293-img0005.jpg)
![Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Expression level of NLRP3, NLRP6, NLRP12, IFI16 & AIM2 inflammasomes in human primary corneal epithelial cells infected with virulent & less-virulent strains of HSV-1. Human primary corneal epithelial cells (hTCEpi cell line) were infected in vitro with HSV-1 laboratory strains McKrae, 17, F, RE or KOS (A,B) or with clinical isolates KOS79 & KOS63 (C,D) at MOI of 1. Two, 8 & 24 h post-infection, hTCEpi cells were harvested & immunoblots of whole cell lysates were performed for expression of NLRP3, NLRP6, NLRP12, IFI16, & AIM2 inflammasomes. Corresponding beta -actin was used as a control. (B,D) Graphs show the kinetics of fold changes in NLRP3, NLRP6, NLRP12, IFI16, & AIM2 inflammasomes expression at 2, 8, & 24 h post-infection with virulent/less-virulent strains of HSV-1 compared to the control & normalized to beta -actin. Results are representative of three experiments. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31367214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76293_rabbit-polyclonal-nalp12-antibody-310202416173536.jpg)
![Western Blot: NALP12 Antibody - BSA Free [NBP1-76293] - Expression levels of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 inflammasomes in mouse corneas following infection with virulent & less-virulent strains of HSV-1. B6 mice (n = 6) were infected ocularly with 2.5 × 105 pfu/eye of HSV-1 laboratory strains McKrae, 17, F, RE or KOS or with clinical isolates KOS79 & KOS63. Mock-infected mice (n = 6) were used as controls. Forty-eight hours post-infection animals were euthanized the corneas were excised & homogenized in lysis buffer, & western blot was performed for expression of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2. Corresponding beta -actin was used as a control. Three western blot were performed using three different corneal lysates harvested from three sets of corneas pooled from 2 infected mice (i.e., 4 corneas per lysate). Results are representative of those three independent experiments. Graphs (mean plus SD) show fold change in expression of NLRP3, NLRP6, NLRP12, IFI16/p204, & AIM2 at 48 h post-infection with virulent/less-virulent strains of HSV-1 compared to the control & normalized to beta -actin. Representative densitometric analysis is shown compared to control. *P < 0.05; **P < 0.01; ***P < 0.001 using Student's t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31367214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76293_rabbit-polyclonal-nalp12-antibody-310202416154635.jpg)
![Immunohistochemistry-Paraffin: NALP12 Antibody [NBP1-85555] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NALP12-Antibody-Immunohistochemistry-Paraffin-NBP1-85555-img0006.jpg "Immunohistochemistry-Paraffin: NALP12 Antibody [NBP1-85555] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: NALP12 Antibody [NBP1-85555] - Staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/NALP12-Antibody-Immunohistochemistry-Paraffin-NBP1-85555-img0004.jpg)
![Immunohistochemistry-Paraffin: NALP12 Antibody [NBP1-85555] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/NALP12-Antibody-Immunohistochemistry-Paraffin-NBP1-85555-img0005.jpg)
![Immunohistochemistry-Paraffin: NALP12 Antibody [NBP1-85555] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/NALP12-Antibody-Immunohistochemistry-Paraffin-NBP1-85555-img0007.jpg)
![Western Blot: NALP12 Antibody [NBP2-87879] - Host: Rabbit. Target Name: NLRP12. Sample Type: Hela Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/NALP12-Antibody-Western-Blot-NBP2-87879-img0001.jpg "Western Blot: NALP12 Antibody [NBP2-87879] - Host: Rabbit. Target Name: NLRP12. Sample Type: Hela Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: NALP12 Overexpression Lysate (Adult Normal) [NBL1-13673] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NALP12.](http://images.novusbio.com/fullsize/NALP12-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13673-img0002.jpg "Western Blot: NALP12 Overexpression Lysate (Adult Normal) [NBL1-13673] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NALP12.")
![Western Blot: NALP12 Antibody [NBP2-93584] - Analysis of extracts of rat spleen, using NALP12 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/NALP12-Antibody-Western-Blot-NBP2-93584-img0001.jpg "Western Blot: NALP12 Antibody [NBP2-93584] - Analysis of extracts of rat spleen, using NALP12 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: NALP12 Antibody [NBP2-94058] - Analysis of extracts of various cell lines, using NALP12 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/NALP12-Antibody-Western-Blot-NBP2-94058-img0001.jpg "Western Blot: NALP12 Antibody [NBP2-94058] - Analysis of extracts of various cell lines, using NALP12 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")