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![Western Blot: EIF3S3 Antibody [NBP1-84870] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/EIF3S3-Antibody-Western-Blot-NBP1-84870-img0007.jpg "Western Blot: EIF3S3 Antibody [NBP1-84870] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunocytochemistry/Immunofluorescence: EIF3S3 Antibody [NBP1-84870] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84870-img0008.jpg)
![Immunohistochemistry-Paraffin: EIF3S3 Antibody [NBP1-84870] - Staining of human duodenum shows strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-Immunohistochemistry-Paraffin-NBP1-84870-img0011.jpg)
![Western Blot: EIF3S3 Antibody [NBP1-84870] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/EIF3S3-Antibody-Western-Blot-NBP1-84870-img0006.jpg)
![Immunohistochemistry-Paraffin: EIF3S3 Antibody [NBP1-84870] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells and cells in molecular layer.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-Immunohistochemistry-Paraffin-NBP1-84870-img0009.jpg)
![Immunohistochemistry-Paraffin: EIF3S3 Antibody [NBP1-84870] - Staining of human cervix shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-Immunohistochemistry-Paraffin-NBP1-84870-img0010.jpg)
![Immunohistochemistry-Paraffin: EIF3S3 Antibody [NBP1-84870] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells and cells in seminiferous ducts.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-Immunohistochemistry-Paraffin-NBP1-84870-img0012.jpg)
![Western Blot: EIF3S3 Antibody (GT35512) [NBP3-13550] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with EIF3S3 antibody [GT35512] (NBP3-13550) diluted at 1:1000.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-GT35512-Western-Blot-NBP3-13550-img0001.jpg "Western Blot: EIF3S3 Antibody (GT35512) [NBP3-13550] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with EIF3S3 antibody [GT35512] (NBP3-13550) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: EIF3S3 Antibody (GT35512) [NBP3-13550] - EIF3S3 antibody [GT35512] detects EIF3S3 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF3S3 protein stained by EIF3S3 antibody [GT35512] (NBP3-13550) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-GT35512-Immunocytochemistry-Immunofluorescence-NBP3-13550-img0002.jpg)
![Immunohistochemistry-Paraffin: EIF3S3 Antibody (GT35512) [NBP3-13550] - Mouse testis. EIF3S3 antibody [35512] (NBP3-13550) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-GT35512-Immunohistochemistry-Paraffin-NBP3-13550-img0003.jpg)
![Knockout Validated: EIF3S3 Antibody [NBP3-03810] - Analysis of extracts from normal (control) and EIF3H knockout (KO) HeLa cells, using EIF3S3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-Knockout-Validated-NBP3-03810-img0001.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: EIF3S3 Antibody [NBP3-03810] - Analysis of extracts from normal (control) and EIF3H knockout (KO) HeLa cells, using EIF3S3 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: EIF3S3 Antibody (PSH04-49) [NBP3-32298] - Western blot analysis of EIF3S3 on different lysates with Mouse anti-EIF3S3 antibody (NBP3-32298) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: MDA-MB-231 cell lysate Lane 9: MDA-MB-468 cell lysate Lane 10: MCF7 cell lysate Lane 11: RAW264.7 cell lysate Lane 12: NIH/3T3 cell lysate Lane 13: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32298_mouse-eif3s3-mab-psh04-49-27820241631672.jpg "Western Blot: EIF3S3 Antibody (PSH04-49) [NBP3-32298] - Western blot analysis of EIF3S3 on different lysates with Mouse anti-EIF3S3 antibody (NBP3-32298) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: MDA-MB-231 cell lysate Lane 9: MDA-MB-468 cell lysate Lane 10: MCF7 cell lysate Lane 11: RAW264.7 cell lysate Lane 12: NIH/3T3 cell lysate Lane 13: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: EIF3S3 Antibody (PSH04-49) [NBP3-32298] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EIF3S3 antibody (NBP3-32298) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32298) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32298_mouse-eif3s3-mab-psh04-49-298202414161894.jpg)
![Western Blot: EIF3S3 Antibody [NBP3-03812] - Analysis of extracts of various cell lines, using EIF3S3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-Western-Blot-NBP3-03812-img0003.jpg "Western Blot: EIF3S3 Antibody [NBP3-03812] - Analysis of extracts of various cell lines, using EIF3S3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Western Blot: EIF3S3 Antibody [NBP3-03812] - Analysis of 200ug extracts of Jurkat cells using EIF3S3 antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-Western-Blot-NBP3-03812-img0002.jpg)
![Western Blot: EIF3S3 Antibody [NBP1-31572] - Sample(30 ug whole cell lysate)A:A431 B:H1299 C:Hep G2 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-Western-Blot-NBP1-31572-img0002.jpg "Western Blot: EIF3S3 Antibody [NBP1-31572] - Sample(30 ug whole cell lysate)A:A431 B:H1299 C:Hep G2 10% SDS PAGE, antibody diluted at 1:1000.")
![Western Blot: EIF3S3 Antibody (3B12) [H00008667-M01] - EIF3S3 monoclonal antibody (M01), clone 3B12 Analysis of EIF3S3 expression in Jurkat.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-3B12-Western-Blot-H00008667-M01-img0004.jpg "Western Blot: EIF3S3 Antibody (3B12) [H00008667-M01] - EIF3S3 monoclonal antibody (M01), clone 3B12 Analysis of EIF3S3 expression in Jurkat.")
![Sandwich ELISA: EIF3S3 Antibody (3B12) [H00008667-M01] - Detection limit for recombinant GST tagged EIF3S3 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-3B12-Sandwich-ELISA-H00008667-M01-img0003.jpg)
![Western Blot: EIF3S3 Antibody (GT2712) [NBP3-13549] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with EIF3S3 antibody [GT2712] (NBP3-13549) diluted at 1:1000.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-GT2712-Western-Blot-NBP3-13549-img0001.jpg "Western Blot: EIF3S3 Antibody (GT2712) [NBP3-13549] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with EIF3S3 antibody [GT2712] (NBP3-13549) diluted at 1:1000.")
![Western Blot: EIF3S3 Overexpression Lysate (Adult Normal) [NBL1-10196] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EIF3S3.](http://images.novusbio.com/fullsize/EIF3S3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10196-img0002.jpg "Western Blot: EIF3S3 Overexpression Lysate (Adult Normal) [NBL1-10196] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EIF3S3.")
![Sandwich ELISA: EIF3S3 Antibody (3C6) [H00008667-M16] - Detection limit for recombinant GST tagged EIF3H is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EIF3S3-Antibody-3C6-Sandwich-ELISA-H00008667-M16-img0001.jpg "Sandwich ELISA: EIF3S3 Antibody (3C6) [H00008667-M16] - Detection limit for recombinant GST tagged EIF3H is 0.3 ng/ml as a capture antibody.")