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![Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H2a-Antibody-Western-Blot-NB100-56346-img0004.jpg "Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346] - Human breast probed with Histone H2A antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Histone-H2a-Antibody-Immunohistochemistry-Paraffin-NB100-56346-img0006.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Analysis of Histone H2A in human PBMC lysate probed with Histone H2A antibody.](http://images.novusbio.com/fullsize/Histone-H2a-Antibody-Western-Blot-NB100-56346-img0002.jpg)
![Western Blot: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H2a-ac-Lys5-Antibody-GT1262-Western-Blot-NBP3-13534-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2a (acetyl Lys5) protein stained by Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (NBP2-20759) diluted at 1:300. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Histone-H2a-ac-Lys5-Antibody-GT1262-Immunocytochemistry-Immunofluorescence-NBP3-13534-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H2a-ac-Lys5-Antibody-GT1262-Immunohistochemistry-Paraffin-NBP3-13534-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H2a-ac-Lys5-Antibody-GT1262-Immunohistochemistry-Paraffin-NBP3-13534-img0003.jpg)
![Western Blot: Histone H2a [ac Lys5] Antibody [NBP2-59261] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2AK5ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left](http://images.novusbio.com/fullsize/Histone-H2a-[ac-Lys5]-Antibody-Western-Blot-NBP2-59261-img0001.jpg "Western Blot: Histone H2a [ac Lys5] Antibody [NBP2-59261] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2AK5ac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left")
![Immunocytochemistry/Immunofluorescence: Histone H2a [ac Lys5] Antibody [NBP2-59261] - HeLa cells were stained with the antibody against H2AK5ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2AK5ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H2a-[ac-Lys5]-Antibody-Immunofluorescence-NBP2-59261-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H2a [ac Lys5] Antibody [NBP2-59261] - ChIP assays were performed using human HeLa cells, the antibody against H2AK5ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H2a-[ac-Lys5]-Antibody-Chromatin-Immunoprecipitation-NBP2-59261-img0003.jpg)
![ELISA: Histone H2a [ac Lys5] Antibody [NBP2-59261] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2AK5ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,000.](http://images.novusbio.com/fullsize/Histone-H2a-[ac-Lys5]-Antibody-ELISA-NBP2-59261-img0002.jpg)
![Dot Blot: Histone H2a [ac Lys5] Antibody [NBP2-59261] - To test the cross reactivity of the antibody against H2AK5ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H2AK5. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H2a-[ac-Lys5]-Antibody-Dot-Blot-NBP2-59261-img0006.jpg)
![Western Blot: Histone H2a [ac Lys9] Antibody (PS01-45) [NBP3-32425] - Western blot analysis of Histone H2a on different lysates with Rabbit anti-Histone H2a antibody (NBP3-32425) at 1/1,000 dilution. Lane 1: Hela whole cell lysate Lane 2: Hela treated with 500 ng/ml TSA for 4 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32425) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32425_rabbit-histone-h2a-ac-lys9-mab-ps01-45-278202415242643.jpg "Western Blot: Histone H2a [ac Lys9] Antibody (PS01-45) [NBP3-32425] - Western blot analysis of Histone H2a on different lysates with Rabbit anti-Histone H2a antibody (NBP3-32425) at 1/1,000 dilution. Lane 1: Hela whole cell lysate Lane 2: Hela treated with 500 ng/ml TSA for 4 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time: 3 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32425) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Histone H2a [ac Lys9] Antibody (PS01-45) [NBP3-32425] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Histone H2a antibody (NBP3-32425) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32425) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32425_rabbit-histone-h2a-ac-lys9-mab-ps01-45-298202414333626.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H2a [ac Lys9] Antibody (PS01-45) [NBP3-32425] - Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H2a with Rabbit anti-Histone H2a antibody (NBP3-32425) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H2a antibody (NBP3-32425) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32425_rabbit-histone-h2a-ac-lys9-mab-ps01-45-2920241114814.jpg)
![Western Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - Analysis was performed on whole cell extracts from HeLa cells (25 ug, lane 1), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H2AK119ub.](http://images.novusbio.com/fullsize/Histone-H2a-[ubiquitylated-Lys119]-Antibody-Western-Blot-NBP2-62793-img0004.jpg "Western Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - Analysis was performed on whole cell extracts from HeLa cells (25 ug, lane 1), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H2AK119ub.")
![Chromatin Immunoprecipitation (ChIP): Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - Assays were performed using human HeLa cells, the antibody against H2AK119ub and optimized PCR primer pairs for qPCR. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H2a-ubiquitylated-Lys119-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-62793-img0001.jpg)
![ELISA: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - To determine the titer of the antibody, an analysis was performed using a serial dilution of the Diagenode antibody against H2AK119ub. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:23,000.](http://images.novusbio.com/fullsize/Histone-H2a-[ubiquitylated-Lys119]-Antibody-ELISA-NBP2-62793-img0002.jpg)
![Dot Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - To test the cross reactivity of the antibody against H2AK119ub, a Dot Blot analysis was performed with peptides containing other histone ubiquitinylations and unmodified sequences. This Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H2a-[ubiquitylated-Lys119]-Antibody-Dot-Blot-NBP2-62793-img0003.jpg)
![Western Blot: Histone H2a [p Ser 129] Antibody (SR1183) [NBP3-21526] - Western blot analysis using NBP3-21526 in Saccharomyces cerevisiae treated with Methyl methanesulfonate.](http://images.novusbio.com/fullsize/nbp3-21526_rabbit-histone-h2a-p-ser-129-mab-sr1183-57202316112156.jpg "Western Blot: Histone H2a [p Ser 129] Antibody (SR1183) [NBP3-21526] - Western blot analysis using NBP3-21526 in Saccharomyces cerevisiae treated with Methyl methanesulfonate.")
![Western Blot: Histone H2a Antibody (S08-1A5) [NBP3-15040] - Western blot detection of Histone H2a in K562, C6 cell lysates using Histone H2a Rabbit mAb (1:1000 diluted). Predicted band size: 14kDa. Observed band size: 14kDa.](http://images.novusbio.com/fullsize/Histone-H2a-Antibody-S08-1A5-Western-Blot-NBP3-15040-img0001.jpg "Western Blot: Histone H2a Antibody (S08-1A5) [NBP3-15040] - Western blot detection of Histone H2a in K562, C6 cell lysates using Histone H2a Rabbit mAb (1:1000 diluted). Predicted band size: 14kDa. Observed band size: 14kDa.")