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Histone H2a [p Ser129] Antibody (SR1183)

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Western Blot: Histone H2a [p Ser 129] Antibody (SR1183) [NBP3-21526] - Western blot analysis using NBP3-21526 in Saccharomyces cerevisiae treated with Methyl methanesulfonate.

Product Details

Summary
Reactivity YeSpecies Glossary
Applications WB
Clone
SR1183
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Histone H2a [p Ser129] Antibody (SR1183) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from yeast phospho-Histone H2a [Ser129] (Uniprot #: P04912)
Modification
p Ser129
Specificity
Detects endogenous levels of total phospho-Histone H2a [Ser129]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
H2AC20
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Histone H2a [p Ser129] Antibody (SR1183)

  • H2A histone family, member Q
  • H2A/q
  • H2A1B
  • H2AFQH2A
  • H2A-GL101
  • HIST1H2AB
  • histone 2, H2ac
  • histone cluster 2, H2ac
  • histone H2A type 2-C
  • Histone H2A
  • Histone H2A/q
  • Histone H2A-GL101
  • histone IIa
  • MGC74460

Background

Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol H2AC20