Histone H2a [p Ser129] Antibody (SR1183) Summary
Additional Information |
Recombinant Monoclonal Antibody |
Immunogen |
A synthesized peptide derived from yeast phospho-Histone H2a [Ser129] (Uniprot #: P04912) |
Modification |
p Ser129 |
Specificity |
Detects endogenous levels of total phospho-Histone H2a [Ser129] |
Isotype |
IgG |
Clonality |
Monoclonal |
Host |
Rabbit |
Gene |
H2AC20 |
Purity |
Affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Western Blot 1:1000-1:2000
|
Theoretical MW |
14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS, pH 7.4, 150mM NaCl, 50% glycerol. |
Preservative |
0.02% Sodium Azide |
Purity |
Affinity purified |
Alternate Names for Histone H2a [p Ser129] Antibody (SR1183)
Background
Changes in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). It has been shown that DNA damage induces monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet -induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair. It occurs after incision of the damaged strand and forms part of the cellular response to UV damage and suggests a role of this modification in DNA repair-induced chromatin remodeling (3).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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