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![Western Blot: RING1 Antibody [NBP1-80830] - Analysis in control (vector only transfected HEK293T lysate) and RING1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/RING1-Antibody-Western-Blot-NBP1-80830-img0007.jpg "Western Blot: RING1 Antibody [NBP1-80830] - Analysis in control (vector only transfected HEK293T lysate) and RING1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: RING1 Antibody [NBP1-80830] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/RING1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80830-img0006.jpg)
![Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RING1-Antibody-Immunohistochemistry-Paraffin-NBP1-80830-img0011.jpg)
![Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830] - Staining of human nasopharynx shows strong nuclear and moderate cytoplasmic positivity in respiratory epithelial cells.](http://images.novusbio.com/fullsize/RING1-Antibody-Immunohistochemistry-Paraffin-NBP1-80830-img0002.jpg)
![Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830] - Staining of human Cerebral cortex shows strong nuclear positivity in neuronal and glial cells.](http://images.novusbio.com/fullsize/RING1-Antibody-Immunohistochemistry-Paraffin-NBP1-80830-img0008.jpg)
![Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830] - Staining of human Colon shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/RING1-Antibody-Immunohistochemistry-Paraffin-NBP1-80830-img0009.jpg)
![Immunohistochemistry-Paraffin: RING1 Antibody [NBP1-80830] - Staining of human Endometrium shows strong nuclear positivity in glandular cells and cells in endometrial stroma.](http://images.novusbio.com/fullsize/RING1-Antibody-Immunohistochemistry-Paraffin-NBP1-80830-img0010.jpg)
![Western Blot: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis using Ring1 mouse mAb against MOLT-4 (1), LNCaP (2), Hela (3), HEK-293 (4) and Jurkat (5) cell lysate.](http://images.novusbio.com/fullsize/RING1-Antibody-8C12F4-Western-Blot-NBP2-37370-img0004.jpg "Western Blot: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis using Ring1 mouse mAb against MOLT-4 (1), LNCaP (2), Hela (3), HEK-293 (4) and Jurkat (5) cell lysate.")
![Immunohistochemistry-Paraffin: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis of rectum cancer tissues using Ring1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/RING1-Antibody-8C12F4-Immunohistochemistry-NBP2-37370-img0007.jpg)
![Flow Cytometry: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis of Hela cells using Ring1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/RING1-Antibody-8C12F4-Flow-Cytometry-NBP2-37370-img0005.jpg)
![Western Blot: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis using Ring1 mAb against human Ring1 recombinant protein. (Expected MW is 44.6 kDa)](http://images.novusbio.com/fullsize/RING1-Antibody-8C12F4-Western-Blot-NBP2-37370-img0002.jpg)
![Western Blot: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis using Ring1 mAb against HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RING1-Antibody-8C12F4-Western-Blot-NBP2-37370-img0003.jpg)
![Immunohistochemistry-Paraffin: RING1 Antibody (8C12F4) [NBP2-37370] - Analysis of cervical cancer tissues using Ring1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/RING1-Antibody-8C12F4-Immunohistochemistry-NBP2-37370-img0006.jpg)
![ELISA: RING1 Antibody (8C12F4) [NBP2-37370] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/RING1-Antibody-8C12F4-ELISA-NBP2-37370-img0001.jpg)
![Western Blot: RING1 Antibody (8E8A2) [NBP2-37319] - Western blot analysis using Ring1 mAb against HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RING1-Antibody-8E8A2-Western-Blot-NBP2-37319-img0003.jpg "Western Blot: RING1 Antibody (8E8A2) [NBP2-37319] - Western blot analysis using Ring1 mAb against HEK293 (1) and Ring1 (AA: 79-263)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: RING1 Antibody (8E8A2) [NBP2-37319] - Immunofluorescence analysis of Hela cells using Ring1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/RING1-Antibody-8E8A2-Immunocytochemistry-Immunofluorescence-NBP2-37319-img0005.jpg)
![Western Blot: RING1 Antibody (8E8A2) [NBP2-37319] - Western blot analysis using Ring1 mAb against human Ring1 recombinant protein. (Expected MW is 44.6 kDa)](http://images.novusbio.com/fullsize/RING1-Antibody-8E8A2-Western-Blot-NBP2-37319-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RING1 Antibody (8E8A2) [NBP2-37319] - Immunofluorescence analysis of A431 cells using Ring1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/RING1-Antibody-8E8A2-Immunocytochemistry-Immunofluorescence-NBP2-37319-img0004.jpg)
![ELISA: RING1 Antibody (8E8A2) [NBP2-37319] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/RING1-Antibody-8E8A2-ELISA-NBP2-37319-img0001.jpg)
![Western Blot: RING1 Antibody (2Q8D5) [NBP3-15893] - Western blot analysis of extracts of various cell lines, using RING1 Rabbit mAb (NBP3-15893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/RING1-Antibody-2Q8D5-Western-Blot-NBP3-15893-img0002.jpg "Western Blot: RING1 Antibody (2Q8D5) [NBP3-15893] - Western blot analysis of extracts of various cell lines, using RING1 Rabbit mAb (NBP3-15893) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: RING1 Antibody (2Q8D5) [NBP3-15893] - Immunohistochemistry of paraffin-embedded human thyroid cancer using RING1 Rabbit mAb (NBP3-15893) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RING1-Antibody-2Q8D5-Immunohistochemistry-Paraffin-NBP3-15893-img0001.jpg)
![Immunoprecipitation: RING1 Antibody (2Q8D5) [NBP3-15893] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug RING1 antibody (NBP3-15893). Western blot was performed from the immunoprecipitate using RING1 antibody (NBP3-15893) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/RING1-Antibody-2Q8D5-Immunoprecipitation-NBP3-15893-img0003.jpg)
![Western Blot: RING1 Antibody (4E8) [H00006015-M07] - Analysis of RING1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/RING1-Antibody-4E8-Western-Blot-H00006015-M07-img0004.jpg "Western Blot: RING1 Antibody (4E8) [H00006015-M07] - Analysis of RING1 expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: RING1 Antibody (4E8) [H00006015-M07] - Analysis of monoclonal antibody to RING1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/RING1-Antibody-4E8-Immunocytochemistry-Immunofluorescence-H00006015-M07-img0002.jpg)
![Sandwich ELISA: RING1 Antibody (4E8) [H00006015-M07] - Detection limit for recombinant GST tagged RING1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RING1-Antibody-4E8-Sandwich-ELISA-H00006015-M07-img0003.jpg)
![Western Blot: RING1 Antibody (1D5) [H00006015-M09] - Analysis of RING1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/RING1-Antibody-1D5-Western-Blot-H00006015-M09-img0004.jpg "Western Blot: RING1 Antibody (1D5) [H00006015-M09] - Analysis of RING1 expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: RING1 Antibody (1D5) [H00006015-M09] - Analysis of monoclonal antibody to RING1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/RING1-Antibody-1D5-Immunocytochemistry-Immunofluorescence-H00006015-M09-img0002.jpg)
![Sandwich ELISA: RING1 Antibody (1D5) [H00006015-M09] - Detection limit for recombinant GST tagged RING1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RING1-Antibody-1D5-Sandwich-ELISA-H00006015-M09-img0003.jpg)
![Western Blot: RING1 Antibody (1F4) [H00006015-M10] - Analysis of RING1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/RING1-Antibody-1F4-Western-Blot-H00006015-M10-img0005.jpg "Western Blot: RING1 Antibody (1F4) [H00006015-M10] - Analysis of RING1 expression in Hela S3 NE (Cat # L013V3).")
![Immunocytochemistry/Immunofluorescence: RING1 Antibody (1F4) [H00006015-M10] - Analysis of monoclonal antibody to RING1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/RING1-Antibody-1F4-Immunocytochemistry-Immunofluorescence-H00006015-M10-img0003.jpg)
![Sandwich ELISA: RING1 Antibody (1F4) [H00006015-M10] - Detection limit for recombinant GST tagged RING1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RING1-Antibody-1F4-Sandwich-ELISA-H00006015-M10-img0004.jpg)
![Immunohistochemistry: RING1 Antibody [NBP2-04111] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- RING1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/RING1-Antibody-Immunohistochemistry-NBP2-04111-img0002.jpg "Immunohistochemistry: RING1 Antibody [NBP2-04111] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- RING1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).")
![Immunoprecipitation: RING1 Antibody [NBP2-04111] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RING1 antibody NBP2-04111used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RING1 was also immunoprecipitated by rabbit anti-RING1 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/RING1-Antibody-Immunoprecipitation-NBP2-04111-img0001.jpg)
![Western Blot: RING1 Antibody (2B3) [H00006015-M03] - Analysis of RING1 expression in RIN-m5F.](http://images.novusbio.com/fullsize/RING1-Antibody-2B3-Western-Blot-H00006015-M03-img0005.jpg "Western Blot: RING1 Antibody (2B3) [H00006015-M03] - Analysis of RING1 expression in RIN-m5F.")
![Western Blot: RING1 Antibody (2B3) [H00006015-M03] - Analysis of RING1 expression in H9c2 (2-1).](http://images.novusbio.com/fullsize/RING1-Antibody-2B3-Western-Blot-H00006015-M03-img0004.jpg)
![Sandwich ELISA: RING1 Antibody (2B3) [H00006015-M03] - Detection limit for recombinant GST tagged RING1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RING1-Antibody-2B3-Sandwich-ELISA-H00006015-M03-img0003.jpg)
![Western Blot: RING1 Antibody [NBP2-20164] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/RING1-Antibody-Western-Blot-NBP2-20164-img0001.jpg "Western Blot: RING1 Antibody [NBP2-20164] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:3000.")
![Immunohistochemistry-Paraffin: RING1 Antibody [NBP2-20164] - Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/RING1-Antibody-Immunohistochemistry-Paraffin-NBP2-20164-img0002.jpg)
![Western Blot: RING1 Antibody (SR1892) [NBP3-22110] - Western blot analysis using NBP3-22110 in Ramos cell lysate.](http://images.novusbio.com/fullsize/nbp3-22110_rabbit-ring1-mab-sr1892-572023152464.jpg "Western Blot: RING1 Antibody (SR1892) [NBP3-22110] - Western blot analysis using NBP3-22110 in Ramos cell lysate.")
![Western Blot: RING1 Antibody (JE34-66) [NBP3-32904] - Western blot analysis of RING1 on different lysates with Rabbit anti-RING1 antibody (NBP3-32904) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: 22RV1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 42 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32904) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32904_rabbit-ring1-mab-je34-66-288202412461653.jpg "Western Blot: RING1 Antibody (JE34-66) [NBP3-32904] - Western blot analysis of RING1 on different lysates with Rabbit anti-RING1 antibody (NBP3-32904) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: 22RV1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 42 kDa Observed band size: 50 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32904) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: RING1 Antibody (JE34-66) [NBP3-32904] - Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-RING1 antibody (NBP3-32904) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32904) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32904_rabbit-ring1-mab-je34-66-298202414333647.jpg)