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![Western Blot: RAB27B Antibody [H00005874-B01P] - Analysis of RAB27B expression in transfected 293T cell line by RAB27B polyclonal antibody. Lane 1: RAB27B transfected lysate(24.09 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RAB27B-Antibody-Western-Blot-H00005874-B01P-img0002.jpg "Western Blot: RAB27B Antibody [H00005874-B01P] - Analysis of RAB27B expression in transfected 293T cell line by RAB27B polyclonal antibody. Lane 1: RAB27B transfected lysate(24.09 KDa). Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: RAB27B Antibody [NBP1-89538] - Staining in human prostate and tonsil tissues . Corresponding RAB27B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/RAB27B-Antibody-Immunohistochemistry-Paraffin-NBP1-89538-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: RAB27B Antibody [NBP1-89538] - Staining in human prostate and tonsil tissues . Corresponding RAB27B RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: RAB27B Antibody [NBP1-89538] - Analysis in control (vector only transfected HEK293T lysate) and RAB27B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/RAB27B-Antibody-Western-Blot-NBP1-89538-img0013.jpg)
![Immunohistochemistry-Paraffin: RAB27B Antibody [NBP1-89538] - Staining of human prostate shows strong apical membrane and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/RAB27B-Antibody-Immunohistochemistry-Paraffin-NBP1-89538-img0014.jpg)
![Western Blot: RAB27B Antibody [NBP1-89538] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/fullsize/RAB27B-Antibody-Western-Blot-NBP1-89538-img0007.jpg)
![Immunohistochemistry-Paraffin: RAB27B Antibody [NBP1-89538] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/RAB27B-Antibody-Immunohistochemistry-Paraffin-NBP1-89538-img0008.jpg)
![Immunohistochemistry-Paraffin: RAB27B Antibody [NBP1-89538] - Staining of human small intestine shows strong positivity in apical membranes in glandular cells.](http://images.novusbio.com/fullsize/RAB27B-Antibody-Immunohistochemistry-Paraffin-NBP1-89538-img0010.jpg)
![Immunohistochemistry-Paraffin: RAB27B Antibody [NBP1-89538] - Staining of human tonsil shows mainly very weak positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/RAB27B-Antibody-Immunohistochemistry-Paraffin-NBP1-89538-img0011.jpg)
![Western Blot: RAB27B Antibody [NBP1-79631] - Tumor cell-released Hsp70/90-expressing EVs induce muscle catabolism in myotubes. Hsp70/90 release by cachexia-inducing tumor cells is dependent on EV release. Rab27a and Rab27b were knocked down by transfecting LLC and C26 cells with control or specific siRNAs. EVs and Hsp70/90 released into conditioned media were quantified after Brij98 treatment. EVs were quantified by AchE. Data (n?=?3) were analyzed by Student t-test. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-00726-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RAB27B-Antibody-Western-Blot-NBP1-79631-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: RAB27B Antibody [NBP1-79631] - Tumor cell-released Hsp70/90-expressing EVs induce muscle catabolism in myotubes. Hsp70/90 release by cachexia-inducing tumor cells is dependent on EV release. Rab27a and Rab27b were knocked down by transfecting LLC and C26 cells with control or specific siRNAs. EVs and Hsp70/90 released into conditioned media were quantified after Brij98 treatment. EVs were quantified by AchE. Data (n?=?3) were analyzed by Student t-test. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-00726-x) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Western Blot: RAB27B Antibody [NBP1-79631] - Rab27b Antibody Titration: 0.5 ug/ml Positive Control: human testis, mouse liver, brain and testis](http://images.novusbio.com/fullsize/RAB27B-Antibody-Western-Blot-NBP1-79631-img0001.jpg)
![Western Blot: RAB27B Antibody [NBP1-79631] - Mouse Heart Lysate 1ug/ml Gel Concentration 12%](http://images.novusbio.com/fullsize/RAB27B-Antibody-Western-Blot-NBP1-79631-img0002.jpg)
![Western Blot: RAB27B Antibody [NBP1-79631] - Tumor cell-released Hsp70/90-expressing EVs are critical to the development of muscle wasting in mice. a Elevation of serum Hsp70/90 in LLC tumor-bearing mice is dependent on EV release. LLC cells transduced with lentivirus encoding Rab27a- & Rab27b-specific shRNA or empty vector were analyzed for knockdown effect by western blotting (top). Mice were then implanted with Rab27-deficient or control LLC cells. In 21 days serum levels of EVs, Hsp70/90 were analyzed. b Rab27-deficient LLC tumor does not induce muscle catabolism in mice. Mice derived from a were analyzed for activity of the catabolic markers in TA. c Rab27-deficient LLC tumor does not induce muscle wasting in mice. Mice derived from a were analyzed for muscle wasting. Scale bar, 100 μm. Data (n = 6) were analyzed by analysis of variance or χ2 analysis (for CSA). * denotes a difference (P < 0.05) Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-017-00726-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-79631_rabbit-polyclonal-rab27b-antibody-310202416163793.jpg)
![Western Blot: RAB27B Antibody [NBP3-35136] - Western blot analysis of lysates from Mouse stomach, using RAB27B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35136_rabbit-rab27b-pab-91220241581249.jpg "Western Blot: RAB27B Antibody [NBP3-35136] - Western blot analysis of lysates from Mouse stomach, using RAB27B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Western Blot: RAB27B Antibody [NBP3-35136] - Western blot analysis of various lysates, using RAB27B Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 25s.](http://images.novusbio.com/fullsize/nbp3-35136_rabbit-rab27b-pab-912202414163091.jpg)
![Immunocytochemistry/Immunofluorescence: RAB27B Antibody [NBP2-97109] - Immunofluorescence staining of RAB27B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RAB27B polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/RAB27B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97109-img0001.jpg "Immunocytochemistry/Immunofluorescence: RAB27B Antibody [NBP2-97109] - Immunofluorescence staining of RAB27B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RAB27B polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cytoplasm.")
![Immunohistochemistry-Paraffin: RAB27B Antibody [NBP2-97109] - Immunochemical staining of human RAB27B in human prostate with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/RAB27B-Antibody-Immunohistochemistry-Paraffin-NBP2-97109-img0002.jpg)
![Western Blot: RAB27B Overexpression Lysate (Adult Normal) [NBL1-15055] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAB27B.](http://images.novusbio.com/fullsize/RAB27B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15055-img0002.jpg "Western Blot: RAB27B Overexpression Lysate (Adult Normal) [NBL1-15055] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAB27B.")
![SDS-Page: Recombinant Human RAB27B Protein [NBP1-50947] - 15 % SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-RAB27B-Protein-SDS-Page-NBP1-50947-img0001.jpg "SDS-Page: Recombinant Human RAB27B Protein [NBP1-50947] - 15 % SDS-PAGE (3ug)")