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![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase, muscle form (GPmm) Protein expression inicvLz-pretreated male and female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40)=35.87; p<0.0001), middle (b; F(7,40)=33.89; p<0.0001), and caudal (c; F(7,40)=9.43; p<0.0001) VMN of V/V, V/INS, Lz/V, and Lz/INS groups of male (M; left-hand side) and female (F; right-hand side) rats. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. For each VMN segment, data depict mean normalized GAD O.D. valuesS.E.M. Results are summarized in the table at bottom. VMN = Ventromedial hypothalamic nucleus; V = Vehicle; LZ = letrozole; INS = insulin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Glycogen phosphorylase- muscle form Antibody-Western Blot-NBP2-16689-img0008.jpg "Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase, muscle form (GPmm) Protein expression inicvLz-pretreated male and female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40)=35.87; p<0.0001), middle (b; F(7,40)=33.89; p<0.0001), and caudal (c; F(7,40)=9.43; p<0.0001) VMN of V/V, V/INS, Lz/V, and Lz/INS groups of male (M; left-hand side) and female (F; right-hand side) rats. *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001. For each VMN segment, data depict mean normalized GAD O.D. valuesS.E.M. Results are summarized in the table at bottom. VMN = Ventromedial hypothalamic nucleus; V = Vehicle; LZ = letrozole; INS = insulin. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883/) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Human breast cancer. PYGM antibody [C1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Glycogen-phosphorylase--muscle-form-Antibody-Immunohistochemistry-Paraffin-NBP2-16689-img0007.jpg)
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/Glycogen-phosphorylase--muscle-form-Antibody-Western-Blot-NBP2-16689-img0006.jpg)
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-western-blot-2762023105814..jpg)
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PYGM antibody [C1C3] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-western-blot-27620231106..jpg)
![Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Mouse muscle. PYGM stained by PYGM antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-immunohistochemistry-paraffin-276202311154..jpg)
![Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] -Analysis of paraffin-embedded zebrafish tissue, using PYGM antibody [C1C3] at 1:300 dilution.](http://images.novusbio.com/fullsize/antibody/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-immunohistochemistry-paraffin-276202311258..jpg)
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase-muscle type (GPmm) Protein expression in icv Lz-pretreated male & female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40) = 35.87; p < 0.0001), middle (b; F(7,40) = 33.89; p < 0.0001), & caudal (c; F(7,40) = 9.43; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-310202415175293.jpg)
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - Region-based patterns of glycogen phosphorylase-muscle type (GPmm) Protein expression in icv Lz-pretreated male & female rats. GPmm protein was measured by Western blot in the rostral (a; F(7,40) = 35.87; p < 0.0001), middle (b; F(7,40) = 33.89; p < 0.0001), & caudal (c; F(7,40) = 9.43; p < 0.0001) VMN of V/V, V/INS, Lz/V, & Lz/INS groups of male (M; left-hand side) & female (F; right-hand side) rats. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. For each VMN segment, data depict mean normalized GAD O.D. values ± S.E.M. Results are summarized in the table at bottom; ↑ & ↓ denote a relative increase or decrease, respectively, between treatment groups; no change (NC) between groups is also indicated Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33238883), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-31020241534310.jpg)
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP2-16689] - ER alpha & ER beta Involvement in Noradrenergic Regulation of VMN GS & GPbb/GPmm Protein Expression. Micropunch-dissected VMN tissue obtained from groups of female rats (n = 6/group) infused into the VMN with Veh or NE after Veh, MPP, or PHTPP pretreatment was analyzed by Western blot for GS (Panel 5A), F(5, 12) = 8.44, p = .0003; GPbb (Panel 5B), F(5, 12) = 12.90, p < .0001; or GPmm (Panel 5C), F(5, 12) = 16.49, p < .0001 protein content. Data show mean normalized protein optical density (O.D.) values ± SEM. *p < .05; **p < .01; ***p < .001. VMN = ventromedial hypothalamic nucleus; GS = glycogen synthase; GPmm = glycogen phosphorylase-muscle type; GPbb = glycogen phosphorylase-brain type; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-16689_rabbit-polyclonal-glycogen-phosphorylase-muscle-form-antibody-310202416121962.jpg)
![Western Blot: Glycogen phosphorylase, muscle form Antibody (2C4) [H00005837-M10] - Analysis of PYGM expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Glycogen-phosphorylase-muscle-form-Antibody-2C4-Western-Blot-H00005837-M10-img0009.jpg "Western Blot: Glycogen phosphorylase, muscle form Antibody (2C4) [H00005837-M10] - Analysis of PYGM expression in NIH/3T3 (Cat # L018V1).")
![Western Blot: Glycogen phosphorylase, muscle form Antibody (2C4) [H00005837-M10] - Analysis of PYGM expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/Glycogen-phosphorylase-muscle-form-Antibody-2C4-Western-Blot-H00005837-M10-img0006.jpg)
![Western Blot: Glycogen phosphorylase, muscle form Antibody (2C4) [H00005837-M10] - Analysis of PYGM expression in HepG2 (Cat # L019V1).](http://images.novusbio.com/fullsize/Glycogen-phosphorylase-muscle-form-Antibody-2C4-Western-Blot-H00005837-M10-img0007.jpg)
![Western Blot: Glycogen phosphorylase, muscle form Antibody (2C4) [H00005837-M10] - Analysis of PYGM expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Glycogen-phosphorylase-muscle-form-Antibody-2C4-Western-Blot-H00005837-M10-img0008.jpg)
![ELISA: Glycogen phosphorylase, muscle form Antibody (2C4) [H00005837-M10] - Detection limit for recombinant GST tagged PYGM is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glycogen-phosphorylase-muscle-form-Antibody-2C4-ELISA-H00005837-M10-img0005.jpg)
![Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-49209] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/Glycogen-phosphorylase--muscle-form-Antibody-Immunohistochemistry-Paraffin-NBP2-49209-img0003.jpg "Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-49209] - Staining of human skin shows low expression as expected.")
![Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-49209] - Staining of human skeletal muscle shows high expression.](http://images.novusbio.com/fullsize/Glycogen-phosphorylase--muscle-form-Antibody-Immunohistochemistry-Paraffin-NBP2-49209-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP2-49209] - Staining in human skeletal muscle and skin tissues using anti-PYGM antibody. Corresponding PYGM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glycogen-phosphorylase-muscle-form-Antibody-Immunohistochemistry-Paraffin-NBP2-49209-img0004.jpg)
![Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP3-14337] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skeletal muscle after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Glycogen phosphorylase- muscle form Antibody-Immunohistochemistry-Paraffin-NBP3-14337-img0001.jpg "Immunohistochemistry-Paraffin: Glycogen phosphorylase, muscle form Antibody [NBP3-14337] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skeletal muscle after heat-induced antigen retrieval.")
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP3-38607] - Western blot analysis of various lysates using Glycogen phosphorylase, muscle form Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38607_rabbit-glycogen-phosphorylase-muscle-form-pab-10122024124390.jpg "Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP3-38607] - Western blot analysis of various lysates using Glycogen phosphorylase, muscle form Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![SDS-Page: Recombinant Human Glycogen phosphorylase, muscle form Protein [H00005837-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005837-Q01-1.jpg "SDS-Page: Recombinant Human Glycogen phosphorylase, muscle form Protein [H00005837-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP1-74149] - Titration: 1.0 ug/ml Positive Control: Mouse Heart.](http://images.novusbio.com/fullsize/Glycogen-phosphorylase-muscle-form-Antibody-Western-Blot-NBP1-74149-img0002.jpg "Western Blot: Glycogen phosphorylase, muscle form Antibody [NBP1-74149] - Titration: 1.0 ug/ml Positive Control: Mouse Heart.")
![ELISA: Mouse Glycogen phosphorylase, muscle form ELISA Kit (Colorimetric) [NBP2-82416] - Samples were spiked with high concentrations of Mouse Glycogen phosphorylase, muscle form and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82416_mouse-glycogen-phosphorylase-muscle-form-elisa-kit-color-132202416221042.jpg "ELISA: Mouse Glycogen phosphorylase, muscle form ELISA Kit (Colorimetric) [NBP2-82416] - Samples were spiked with high concentrations of Mouse Glycogen phosphorylase, muscle form and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Glycogen phosphorylase, muscle form ELISA Kit (Colorimetric) [NBP2-82416] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82416_mouse-glycogen-phosphorylase-muscle-form-elisa-kit-color-43202497367.jpg)
![ELISA: Human Glycogen phosphorylase, muscle form ELISA Kit (Colorimetric) [NBP2-82521] - Samples were spiked with high concentrations of Human Glycogen phosphorylase, muscle form and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82521_human-glycogen-phosphorylase-muscle-form-elisa-kit-color-132202416232328.jpg "ELISA: Human Glycogen phosphorylase, muscle form ELISA Kit (Colorimetric) [NBP2-82521] - Samples were spiked with high concentrations of Human Glycogen phosphorylase, muscle form and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Glycogen phosphorylase, muscle form ELISA Kit (Colorimetric) [NBP2-82521] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82521_human-glycogen-phosphorylase-muscle-form-elisa-kit-color-432024955044.jpg)
![ELISA: Rat Glycogen phosphorylase, muscle form ELISA Kit (Colorimetric) [NBP2-82502] - Samples were spiked with high concentrations of Rat Glycogen phosphorylase, muscle form and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82502_rat-glycogen-phosphorylase-muscle-form-elisa-kit-color-132202416254086.jpg "ELISA: Rat Glycogen phosphorylase, muscle form ELISA Kit (Colorimetric) [NBP2-82502] - Samples were spiked with high concentrations of Rat Glycogen phosphorylase, muscle form and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Glycogen phosphorylase, muscle form ELISA Kit (Colorimetric) [NBP2-82502] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82502_rat-glycogen-phosphorylase-muscle-form-elisa-kit-color-4320249133273.jpg)
![Western Blot: Glycogen phosphorylase, muscle form Overexpression Lysate (Adult Normal) [NBL1-15022] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glycogen phosphorylase, muscle form.](http://images.novusbio.com/fullsize/Glycogen-phosphorylase-muscle-form-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15022-img0002.jpg "Western Blot: Glycogen phosphorylase, muscle form Overexpression Lysate (Adult Normal) [NBL1-15022] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Glycogen phosphorylase, muscle form.")
![Sandwich ELISA: Glycogen phosphorylase, muscle form Antibody (3D8) [H00005837-M02] - Detection limit for recombinant GST tagged PYGM is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Glycogen-phosphorylase-muscle-form-Antibody-3D8-Sandwich-ELISA-H00005837-M02-img0002.jpg "Sandwich ELISA: Glycogen phosphorylase, muscle form Antibody (3D8) [H00005837-M02] - Detection limit for recombinant GST tagged PYGM is approximately 1ng/ml as a capture antibody.")