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![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87156] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-UIMC1 antibody NBP1-87156 (A) shows similar protein distribution across tissues to independent antibody NBP1-87157 (B).](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-87156-img0016.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87156] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-UIMC1 antibody NBP1-87156 (A) shows similar protein distribution across tissues to independent antibody NBP1-87157 (B).")
![<b>Independent Antibodies Validation. </b>Western Blot: RAP80 Antibody [NBP1-87156] - Analysis in human cell line HDLM-2.](http://images.novusbio.com/fullsize/RAP80-Antibody-Western-Blot-NBP1-87156-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: RAP80 Antibody [NBP1-87156] - EHMT1 and EHMT2 are required for accumulation of ubiquitin conjugates and repair factors at DNA damage sites. ICC/IF analysis of U2OS cells transfected with indicated siRNA, and co-immunostained with indicated antibodies at 2 h after exposure to neocarzinostatin (NCS, 50 ng/ml for 15 min). Representative images of each treated or control cells shown. DNA damage induced foci are quantified as the % of cells with more than 5 large foci in nuclei after background subtraction, each based on at least 150 cells, 3 independent experiments (right). Error bars represent standard deviation (SD). Statistical significance was calculated using two-tailed, unpaired t-test compared with control cells; *P < 0.0001. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-29239-3), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87156-img0018.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87156] - Staining of human testis.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-87156-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: RAP80 Antibody [NBP1-87156] - Staining of human cell line U-2 OS shows localization to nucleus & nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87156-img0007.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87156] - Staining of human cerebral cortex using Anti-UIMC1 antibody.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-87156-img0009.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87156] - Staining of human colon using Anti-UIMC1 antibody.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-87156-img0011.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87156] - Staining of human lymph node using Anti-UIMC1 antibody.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-87156-img0012.jpg)
![Immunocytochemistry/ Immunofluorescence: RAP80 Antibody [NBP1-87156] - EHMT1 & EHMT2 are required for accumulation of ubiquitin conjugates & repair factors at DNA damage sites. (a,b) Immunofluorescence analysis of U2OS cells (a) & MCF7 (b) cells transfected with indicated siRNA, & co-immunostained with indicated antibodies at 2 h after exposure to neocarzinostatin (NCS, 50 ng/ml for 15 min). A representative image of each treated or control cells is shown, as indicated. DNA damage induced foci are quantified as the percentage of cells with more than 5 large foci in nuclei after background subtraction, each based on at least 150 cells from three independent experiments (right). Error bars represent standard deviation (SD). Statistical significance was calculated using two-tailed, unpaired t-test compared with control cells; *P < 0.0001. The knockdown efficiencies with individual siRNAs against EHMT1 & EHMT2 are shown in Fig. S3a,b. Scale bar, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30022091), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87156_rabbit-polyclonal-rap80-antibody-310202416121918.jpg)
![Immunocytochemistry/ Immunofluorescence: RAP80 Antibody [NBP1-87156] - NHEJ pathway inhibition rescue HR & cell survival in FA cells. (A) Representative images of pDNA-PKcs (red) & MRE11 (green) foci in nuclei (DAPI stained, blue) of FANCC-corrected (PD331 corr) or FANCC-mutated (PD331 FANCC−/−) cells in which 53BP1 was depleted by siRNA White line: 2 μm. (B) Histogram presents the frequency of MRE11-positive FANCC−/− cells 48 h after 53BP1 downregulation by siRNA transfection. The presented data are the mean of three independent experiments; error bars indicate S.D. *** indicates P < 0.001 using a Student's t-test. (C) Representative images of pDNA-PKcs (red), 53BP1 (green), RAP80 (red) or MRE11 (green) foci in nuclei (DAPI stained, blue) of FANCC-deficient cells (PD331 FANC−/−) treated with DMSO or with a DNA-PK specific inhibitor. The cells were treated with DNA-PK inhibitor (DNA-PKi 10 μm) for 2 h before MMC exposure (200 ng/ml). White line: 2 μm. (D & E) Clonogenic survival of FANCC-proficient (D, PD331 corr) or -mutated (E, PD331 FANCC−/−) cells after 53BP1 depletion by siRNA and/or DNA-PK inhibition. The cells were treated with MMC at the indicated doses. The presented data are the mean of three independent experiments; error bars indicate S.D. * indicates P < 0.05 using a Student's t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26446986), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-87156_rabbit-polyclonal-rap80-antibody-31020241611352.jpg)
![Western Blot: RAP80 Antibody [NBP1-87157] - Analysis in control (vector only transfected HEK293T lysate) and UIMC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/RAP80-Antibody-Western-Blot-NBP1-87157-img0008.jpg "Western Blot: RAP80 Antibody [NBP1-87157] - Analysis in control (vector only transfected HEK293T lysate) and UIMC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: RAP80 Antibody [NBP1-87157] - Staining of human cell line U-2 OS shows localization to nuclear bodies.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87157-img0007.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87157] - Staining of human colon.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-87157-img0012.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87157] - Staining of human testis shows strong nuclear positivity in seminiferus tubules.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-87157-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87157] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-UIMC1 antibody NBP1-87157 (A) shows similar protein distribution across tissues to independent antibody NBP1-87156 (B).](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-87157-img0009.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87157] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-87157-img0010.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87157] - Staining of human lymph node.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-87157-img0011.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-87157] - Staining of human testis.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-87157-img0013.jpg)
![Western Blot: RAP80 Antibody [NB100-2346] - Detection of Human RAP80 on HeLa whole cell lysate using NB100-2346. RAP80 was also immunoprecipitated using rabbit anti-RAP80 antibody NB100-2347.](http://images.novusbio.com/fullsize/RAP80-Antibody-Western-Blot-NB100-2346-img0001.jpg "Western Blot: RAP80 Antibody [NB100-2346] - Detection of Human RAP80 on HeLa whole cell lysate using NB100-2346. RAP80 was also immunoprecipitated using rabbit anti-RAP80 antibody NB100-2347.")
![Western Blot: RAP80 Antibody [NBP1-76828] - 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/RAP80-Antibody-Western-Blot-NBP1-76828-img0002.jpg "Western Blot: RAP80 Antibody [NBP1-76828] - 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: RAP80 Antibody [NBP1-76828] - Immunofluorescence of RAP80 in Human Spleen cells with RAP80 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76828-img0005.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-76828] - Human spleen tissue with RAP80 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-76828-img0001.jpg)
![Immunohistochemistry: RAP80 Antibody - BSA Free [NBP1-76828] - Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76828_rabbit-polyclonal-rap80-antibody-271120241958415.jpg)
![Immunohistochemistry: RAP80 Antibody - BSA Free [NBP1-76828] - Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76828_rabbit-polyclonal-rap80-antibody-2711202420105816.jpg)
![Immunocytochemistry/ Immunofluorescence: RAP80 Antibody - BSA Free [NBP1-76828] - Immunofluorescence of RAP80 in Human Spleen cells with RAP80 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76828_rabbit-polyclonal-rap80-antibody-2711202419201538.jpg)
![Immunocytochemistry/ Immunofluorescence: RAP80 Antibody - BSA Free [NBP1-76828] - Immunofluorescence of RAP80 in Human Spleen cells with RAP80 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76828_rabbit-polyclonal-rap80-antibody-2711202419553042.jpg)
![Western Blot: RAP80 Antibody [NBP1-76829] - 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/RAP80-Antibody-Western-Blot-NBP1-76829-img0002.jpg "Western Blot: RAP80 Antibody [NBP1-76829] - 293 cell lysate in (A) the absence and (B) presence of blocking peptide with RAP80 antibody at 1 ug/ml.")
![Immunocytochemistry/Immunofluorescence: RAP80 Antibody [NBP1-76829] - Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76829-img0003.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-76829] - Human spleen tissue with RAP80 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-76829-img0001.jpg)
![Immunohistochemistry: RAP80 Antibody - BSA Free [NBP1-76829] - Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76829_rabbit-polyclonal-rap80-antibody-2711202419562176.jpg)
![Immunocytochemistry/ Immunofluorescence: RAP80 Antibody - BSA Free [NBP1-76829] - Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76829_rabbit-polyclonal-rap80-antibody-2711202420333845.jpg)
![Immunocytochemistry/ Immunofluorescence: RAP80 Antibody - BSA Free [NBP1-76829] - Immunofluorescence of RAP80 in human spleen tissue with RAP80 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76829_rabbit-polyclonal-rap80-antibody-2711202419432260.jpg)
![Immunohistochemistry: RAP80 Antibody - BSA Free [NBP1-76829] - Immunohistochemistry of RAP80 in human spleen tissue with RAP80 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76829_rabbit-polyclonal-rap80-antibody-2711202419261884.jpg)
![Western Blot: RAP80 Antibody [NB100-2347] - Detection of Human RAP80 on HeLa whole cell lysate. RAP80 was also immunoprecipitated using rabbit anti-RAP80 antibody NB100-2346.](http://images.novusbio.com/fullsize/RAP80-Antibody-Western-Blot-NB100-2347-img0003.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: RAP80 Antibody [NB100-2347] - Detection of Human RAP80 on HeLa whole cell lysate. RAP80 was also immunoprecipitated using rabbit anti-RAP80 antibody NB100-2346.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: RAP80 Antibody [NB100-2347] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RAP80 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-NB100-2347-img0006.jpg)
![Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-45228] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/RAP80-Antibody-Immunohistochemistry-Paraffin-NBP1-45228-img0001.jpg "Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-45228] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: RAP80 Antibody (SR2242) [NBP3-21834] - Western blot analysis using NBP3-21834 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21834_rabbit-rap80-mab-sr2242-57202317161851.jpg "Western Blot: RAP80 Antibody (SR2242) [NBP3-21834] - Western blot analysis using NBP3-21834 in 293T cell lysate.")
![Western Blot: RAP80 Overexpression Lysate (Adult Normal) [NBP2-07057] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAP80.](http://images.novusbio.com/fullsize/RAP80-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07057-img0001.jpg "Western Blot: RAP80 Overexpression Lysate (Adult Normal) [NBP2-07057] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAP80.")