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![Western Blot: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear extract (50 and 15 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Western-Blot-NB600-248-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear extract (50 and 15 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: KMT2A/MLL Antibody [NB600-248] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-248 used for IP at 6 ug per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 antibody NB600-249. For blotting immunoprecipitated MLL1, NB600-248 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Immunoprecipitation-NB600-248-img0009.jpg)
![Western Blot: KMT2A/MLL Antibody [NB600-256] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Western-Blot-NB600-256-img0012.jpg "Western Blot: KMT2A/MLL Antibody [NB600-256] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Flow Cytometry: KMT2A/MLL Antibody [NB600-256] - Flow cytometry testing of KMT2A/MLL antibody on murine WT and Mll1-/- CD4 T cells that were stimulated in-vitro with anti-CD3/ -CD28 antibodies. Image submitted by our verified customer (see Review section for additional details).](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Flow-Cytometry-NB600-256-img0010.jpg)
![Chromatin Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - A. 10 ug of was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256).](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Chromatin-Immunoprecipitation-NB600-256-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: KMT2A/MLL Antibody [NB600-256] - Detection of human MLL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-256 used for IP at 3 ug per reaction. MLL1 was also immunoprecipitated by another rabbit anti-MLL1 antibody. For blotting immunoprecipitated MLL1, NB600-256 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Immunoprecipitation-NB600-256-img0013.jpg)
![Western Blot: KMT2A/MLL Antibody [NB600-249] - Samples: Nuclear Extract (15 and 50 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Western-Blot-NB600-249-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: KMT2A/MLL Antibody [NB600-249] - Samples: Nuclear Extract (15 and 50 ug) from HeLa, 293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-MLL1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: KMT2A/MLL Antibody [NB600-249] - Samples: Nuclear Extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody NB600-249 used for IP at 6 ug per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 antibody NB600-248. For blotting immunoprecipitated MLL1, NB600-249 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Immunoprecipitation-NB600-249-img0003.jpg)
![Western Blot: KMT2A/MLL Antibody (BL-175-7E8) [NBP2-76397] -Whole cell lysate (50 ug) from HEK293T, MCF-7, A-549, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MLL1 recombinant monoclonal antibody [BL-175-7E8] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/nbp2-76397_rabbit-monoclonal-kmt2a-mll-antibody-bl-175-7e8-western-blot-1642024111131..jpg "Western Blot: KMT2A/MLL Antibody (BL-175-7E8) [NBP2-76397] -Whole cell lysate (50 ug) from HEK293T, MCF-7, A-549, and SW620 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MLL1 recombinant monoclonal antibody [BL-175-7E8] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunoprecipitation: KMT2A/MLL Antibody (BL-175-7E8) [NBP2-76397] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit antiMLL1 recombinant monoclonal antibody [BL-175-7E8used for IP at 6 ul/mg lysate. MLL1 was also immunoprecipitated by a previous lot of this antibody and another antibody against a different epitope of MLL1. For blotting immunoprecipitated MLL1, was used at 1:1000. Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/nbp2-76397_rabbit-monoclonal-kmt2a-mll-antibody-bl-175-7e8-immunoprecipitation-1642024111353..jpg)
![Immunocytochemistry/Immunofluorescence: KMT2A/MLL Antibody [NBP2-55237] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/KMT2A-MLL-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55237-img0001.jpg "Immunocytochemistry/Immunofluorescence: KMT2A/MLL Antibody [NBP2-55237] - Staining of human cell line A-431 shows localization to nucleoplasm.")