| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-600SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78259SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3455-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3324-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52475-0.025mg | |||
![Knockdown Validated: GLI-1 Antibody - BSA Free [NB600-600] - GLI-1 Antibody [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo and in vitro. (E) IHC staining for Ki-67, -SMA, Gli-1 and LYVE1 in tumour sections from mice in the two groups. Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31012844/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunohistochemistry-Paraffin-NB600-600-img0005.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: GLI-1 Antibody - BSA Free [NB600-600] - GLI-1 Antibody [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo and in vitro. (E) IHC staining for Ki-67, -SMA, Gli-1 and LYVE1 in tumour sections from mice in the two groups. Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31012844/) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Analysis of a FFPE mouse brain section using GLI-1 antibody at 1:600 dilution.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunohistochemistry-Paraffin-NB600-600-img0003.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Staining in canine hair follicle. Image from a verified customer review.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunohistochemistry-Paraffin-NB600-600-img0004.jpg)
![Flow (Intracellular): GLI-1 Antibody [NB600-600] - Staining for GLI-1 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-1 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Flow-Intracellular-NB600-600-img0002.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600] - Paracrine signaling of tumour-derived SHH in CAFs promotes lymphangiogenesis in vivo(A) Growth properties of subcutaneous tumours in mice derived from Caov3 SHH-OE or Caov3 NC cells alone or co-injected with CAFs. Kaplan-Meier plot of time to tumour progression, as evidenced by survival rates over time (n=5). (B) Bright field images of subcutaneous tumours from each group (n=5) 20 days after tumour implantation. (C) Graphical representation of the weights of subcutaneous tumours from each group 20 days after tumour implantation. (D) Immunohistochemical staining for Ki-67, alpha -SMA, Gli-1 & LYVE1 in tumour sections from mice in each group. (E) Graphical representation of the number of LYVE1+ vessels counted from IHC images in panel D. (*P<0.05, **P<0.01, ***P<0.001). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.18621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-600_rabbit-polyclonal-gli-1-antibody-31020241618947.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody - BSA Free [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo & in vitro(A) Representative images & statistical analyses of migration of LECs in a co-culture invasion system with CAFs stably transfected with shNC or Gli-shRNA lentivirus in the presence of rSHH (1 μg/ml). (B) Representative images & statistical analyses of capillary tube formation of LECs treated with supernatants from cultures of CAFs stably transfected with shNC or Gli-shRNA lentivirus in the presence of rSHH (1 μg/ml). (C) Bright field images & graphical representation of the weights of tumours of subcutaneous tumours from mice (n=5) bearing Caov3 SHH-OE cells co-injected with CAFs stably transfected with shNC or Gli-shRNA lentivirus 20 days after tumour implantation. (D) Growth properties of subcutaneous tumours in mice derived from Caov3 SHH-OE cells co-injected with CAFs stably transfected with shNC or Gli-shRNA lentivirus. Kaplan-Meier plot of time to tumour progression, as evidenced by survival rates over time. (E) IHC staining for Ki-67, alpha -SMA, Gli-1 & LYVE1 in tumour sections from mice in the two groups. (F) Graphical representation of the number of LYVE1+ vessels counted from IHC images in panel E. (**P<0.01, ***P<0.001). Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.18621), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-600_rabbit-polyclonal-gli-1-antibody-310202416175276.jpg)
![Immunohistochemistry: GLI-1 Antibody [NBP1-78259] - In vivo GLI1 expression after intratumoral administration of NVP-LDE225. LOX OMVI human melanoma cells were injected s.c into both flanks. Tumors were treated intratumorally on daily basis with vehicle (A & B) or NVP-LDE225 (C & D). Immunofluorescent microscopy of GLI1 was performed on isolated tumor tissues. GLI1 staining was performed by overnight incubation of sections at 4C with rabbit anti-GLI-1 polyclonal Ab or isotype control (A & C) followed by an 1 hr-incubation with AF488 Donkey IgG, anti-rabbit at RT (green). Counterstaining of nuclei was performed with propidium iodide (red). Pictures were taken on a confocal laser-scanning microscope system (LSM 410; Zeiss). Yellow color corresponds to double positive (anti-GLI1 and propidium iodide) nuclear staining. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0069064) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunohistochemistry-NBP1-78259-img0009.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry: GLI-1 Antibody [NBP1-78259] - In vivo GLI1 expression after intratumoral administration of NVP-LDE225. LOX OMVI human melanoma cells were injected s.c into both flanks. Tumors were treated intratumorally on daily basis with vehicle (A & B) or NVP-LDE225 (C & D). Immunofluorescent microscopy of GLI1 was performed on isolated tumor tissues. GLI1 staining was performed by overnight incubation of sections at 4C with rabbit anti-GLI-1 polyclonal Ab or isotype control (A & C) followed by an 1 hr-incubation with AF488 Donkey IgG, anti-rabbit at RT (green). Counterstaining of nuclei was performed with propidium iodide (red). Pictures were taken on a confocal laser-scanning microscope system (LSM 410; Zeiss). Yellow color corresponds to double positive (anti-GLI1 and propidium iodide) nuclear staining. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pone.0069064) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: GLI-1 Antibody [NBP1-78259] - Analysis of GLI-1 on partial recombinant GLI-1 protein (molecular weight of partial recombinant protein is 37 kDa).](http://images.novusbio.com/fullsize/GLI-1-Antibody-Western-Blot-NBP1-78259-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody [NBP1-78259] - GLI1 antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78259-img0006.jpg)
![Immunohistochemistry: GLI-1 Antibody [NBP1-78259] - Immunostaining of GLI-1 (green, indicated by arrows) in the suture mesenchyme of Bmpr1-alpha-fl/fl (control) and GLI1-CreERT2; Bmpr1-alpha-fl/fl (mutant) mice 2 weeks post induction (2 wpt). The inset shows the boxed region magnified. T tests were performed. *P < 0.05; **P < 0.01. Broken lines indicate the outline of the suture. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41413-018-0031-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunohistochemistry-NBP1-78259-img0010.jpg)
![Immunohistochemistry: GLI-1 Antibody [NBP1-78259] - Analysis of GLI-1 in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunohistochemistry-NBP1-78259-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: GLI-1 Antibody - BSA Free [NBP1-78259] - In vivo GLI1 expression after intratumoral administration of NVP-LDE225.LOX OMVI human melanoma cells were injected s.c into both flanks as mentioned above. Tumors were treated intratumorally on daily basis with vehicle (A & B) or NVP-LDE225 (C & D). Immunofluorescent microscopy of GLI1was performed on isolated tumor tissues. GLI1 staining was performed by overnight incubation of sections at 4°C with rabbit anti-human polyclonal Ab (B & D, NBP1-78259, Novus Biologicals, Littleton, CO) or isotype control (A & C) followed by an 1 hr-incubation with Alexa Fluor® 488 Donkey IgG, anti-rabbit (A21206, Invitrogen, Carlsbad, CA) at RT (green). Counterstaining of nuclei was performed with propidium iodide (red). Pictures were taken on a confocal laser-scanning microscope system (LSM 410; Zeiss). Yellow color corresponds to double positive (anti-GLI1 & propidium iodide) nuclear staining. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23935925), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78259_rabbit-polyclonal-gli-1-antibody-3102024161607.jpg)
![Western Blot: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1.](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Western-Blot-NBP2-45872-img0011.jpg "Western Blot: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI1.")
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human placenta tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0010.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0001.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0002.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0003.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0004.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0005.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0006.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0007.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0008.jpg)
![Immunohistochemistry: GLI-1 Antibody (4E2) [NBP2-45872] - Analysis of Human testicular cancer tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/GLI-1-Antibody-4E2-Immunohistochemistry-NBP2-45872-img0009.jpg)
![Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against human GLI1 (AA: 284-449) recombinant protein. (Expected MW is 43.1 kDa)](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Western-Blot-NBP2-52475-img0005.jpg "Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against human GLI1 (AA: 284-449) recombinant protein. (Expected MW is 43.1 kDa)")
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of Hela cells using GLI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Immunofluorescence-NBP2-52475-img0002.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of esophageal cancer tissues using GLI1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Immunohistochemistry-NBP2-52475-img0004.jpg)
![Flow Cytometry: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of HepG2 cells using GLI1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Flow-Cytometry-NBP2-52475-img0001.jpg)
![Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against HEK293 (1) and GLI1 (AA: 284-449)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Western-Blot-NBP2-52475-img0006.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of ovarian cancer tissues using GLI1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Immunohistochemistry-NBP2-52475-img0003.jpg)
![ELISA: GLI-1 Antibody (1B9F8) [NBP2-52475] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-ELISA-NBP2-52475-img0008.jpg)
![Western Blot: GLI-1 Antibody [NBP2-24662] - Analysis of human kidney lysate using 1) pre-immune sera at 1:5000 dilution and 2) GLI-1 antibody at 1 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Western-Blot-NBP2-24662-img0006.jpg "Western Blot: GLI-1 Antibody [NBP2-24662] - Analysis of human kidney lysate using 1) pre-immune sera at 1:5000 dilution and 2) GLI-1 antibody at 1 ug/ml. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody [NBP2-24662] - staining of GLI-1 at 10 ug/ml. Top panel: C666-1 malignant human nasopharyngel carcinoma cell line. Bottom panel: OKF6 tert-1 human oral epithelial cell line. GLI-1 was more highly expressed in the C666-1 than in the OKF6 tert-1 cells. See application notes for protocol details.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24662-img0004.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody [NBP2-24662] - Formalin-fixed, paraffin-embedded human brain tissue stained with an isotype control antibody (top) and GLI-1 antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/GLI-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24662-img0005.jpg)
![Western Blot: GLI-1 Antibody (JF09-08) [NBP2-66867] - Western blot analysis of GLI-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: SH-SY5Y cell lysate](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Western-Blot-NBP2-66867-img0007.jpg "Western Blot: GLI-1 Antibody (JF09-08) [NBP2-66867] - Western blot analysis of GLI-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: SH-SY5Y cell lysate")
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867] - Staining Gli1 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Immunocytochemistry-Immunofluorescence-NBP2-66867-img0003.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody (JF09-08) [NBP2-66867] - Analysis of paraffin-embedded rat testis tissue using anti-Gli1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Immunohistochemistry-Paraffin-NBP2-66867-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867] - Staining Gli1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Immunocytochemistry-Immunofluorescence-NBP2-66867-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (JF09-08) [NBP2-66867] - Staining Gli1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Immunocytochemistry-Immunofluorescence-NBP2-66867-img0002.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody (JF09-08) [NBP2-66867] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-Gli1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GLI-1-Antibody-JF09-08-Immunohistochemistry-Paraffin-NBP2-66867-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (CL12191) [NBP3-15131] -Staining of RH-30 cells using the Anti-GLI1 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/antibody/nbp3-15131_mouse-gli-1-mab-cl12191-immunocytochemistry-immunofluorescence-41020238465..jpg "Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (CL12191) [NBP3-15131] -Staining of RH-30 cells using the Anti-GLI1 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).")
![Western Blot: GLI-1 Antibody (HL247) [NBP3-13667] - Non-transfected (-) and transfected (+) RMS13 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GLI-1 antibody [HL247] (NBP3-13667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GLI-1-Antibody-HL247-Western-Blot-NBP3-13667-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GLI-1 Antibody (HL247) [NBP3-13667] - Non-transfected (-) and transfected (+) RMS13 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GLI-1 antibody [HL247] (NBP3-13667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GLI-1 Antibody (HL247) [NBP3-13667] - GLI-1 antibody [HL247] detects GLI-1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. GLI-1 stained by GLI-1 antibody [HL247] (NBP3-13667) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GLI-1-Antibody-HL247-Immunohistochemistry-Paraffin-NBP3-13667-img0002.jpg)
![Western Blot: GLI-1 Antibody (HL247) [NBP3-13667] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with GLI-1 antibody [HL27] (NBP3-13667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GLI-1-Antibody-HL247-Western-Blot-NBP3-13667-img0001.jpg)
![Western Blot: GLI-1 Antibody (HL247) [NBP3-13667] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with GLI-1 antibody [HL247] (NBP3-13667) diluted at 1:1000000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GLI-1-Antibody-HL247-Western-Blot-NBP3-13667-img0003.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody (HL247) [NBP3-13667] - GLI-1 antibody [HL27] detects GLI-1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.GLI-1 stained by GLI-1 antibody [HL27] (NBP3-13667) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13667_rabbit-gli-1-mab-hl247-9102024205649.jpg)
![Western Blot: GLI-1 Antibody (GT249) [NBP3-13674] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with GLI-1 antibody [GT249] (NBP3-13674) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/GLI-1-Antibody-GT249-Western-Blot-NBP3-13674-img0001.jpg "Western Blot: GLI-1 Antibody (GT249) [NBP3-13674] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with GLI-1 antibody [GT249] (NBP3-13674) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: GLI-1 Antibody (GT249) [NBP3-13674] - GLI-1 antibody [GT249] detects GLI-1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. GLI-1 stained by GLI-1 antibody [GT249] (NBP3-13674) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GLI-1-Antibody-GT249-Immunohistochemistry-Paraffin-NBP3-13674-img0002.jpg)