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![Western Blot: Rictor Antibody (7B3) [NBP1-51645] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Western-Blot-NBP1-51645-img0008.jpg "Western Blot: Rictor Antibody (7B3) [NBP1-51645] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates.")
![Immunocytochemistry/Immunofluorescence: Rictor Antibody (7B3) [NBP1-51645] - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Immunocytochemistry-Immunofluorescence-NBP1-51645-img0006.jpg)
![Immunohistochemistry-Paraffin: Rictor Antibody (7B3) [NBP1-51645] - Analysis of paraffin-embedded Human breast carcinoma using Rictor antibody. Antibody dilution 1:200. Image from verified customer review](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Immunohistochemistry-Paraffin-NBP1-51645-img0016.jpg)
![Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Flow-Cytometry-NBP1-51645-img0015.jpg)
![Immunohistochemistry-Paraffin: Rictor Antibody (7B3) [NBP1-51645] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Immunohistochemistry-Paraffin-NBP1-51645-img0007.jpg)
![Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Flow-Cytometry-NBP1-51645-img0005.jpg)
![Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Flow-Cytometry-NBP1-51645-img0012.jpg)
![Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on Jurkat cells with Rictor Antibody (7B3) NBP1-51645AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Flow-Cytometry-NBP1-51645-img0013.jpg)
![Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Flow-Cytometry-NBP1-51645-img0014.jpg)
![Western Blot: Rictor Antibody [NB100-612] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Rictor-Antibody-Western-Blot-NB100-612-img0020.jpg "Western Blot: Rictor Antibody [NB100-612] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Genetic Strategies Validation. </b>Knockdown Validated: Rictor Antibody [NB100-612] - SERCA2A expression is regulated by mTORC2. Rictor silencing was induced by cell transfection with rictor siRNA and then, cells were stimulated with IGF-1 for 24 hours in presence or absence of E2. mTORC2 downregulation was confirmed by abolished Akt-pS473 and resulted in decreased SERCA2A protein expression. Akt-pT308, mTOR and raptor were not negatively affected by rictor silencing. Pictured are representative western blots. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123385), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rictor-Antibody-Western-Blot-NB100-612-img0025.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Rictor Antibody [NB100-612] - Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rictor antibody NB100-612 (lot NB100-612-4) used for IP at 3 ug per reaction. Rictor was also immunoprecipitated by a previous lot of this antibody (lot NB100-612-3) and rabbit anti-Rictor antibody NB100-611. For blotting immunoprecipitated Rictor, NB100-612 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunoprecipitation-NB100-612-img0022.jpg)
![Western Blot: Rictor Antibody [NB100-612] - mTOR complex protein expressions & rictor phosphorylation at Ser1235. Cardiomyocytes were pretreated for 30 min with 20 nM rapamycin, then stimulated with 10 nM IGF-1 in presence/absence of 10 nM E2 for 24 h. Western blots for GSK3beta-pS9, GSK3beta, rictor pS1235 and rictor. At least 3 independent experiments indicate that rictor phosphorylation at S1235 by GSK-3beta downregulates mTORC2 activity. IGF-1 induced strong phosphorylation of GSK-3beta at Ser9 in the absence and presence of E2. Rapamycin pretreatment reduced phosphorylation in E2 co-treated cardiomyocytes, indicating increased activity of GSK-3beta, which was not associated with increased phosphorylation of rictor at S1235. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123385), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rictor-Antibody-Western-Blot-NB100-612-img0024.jpg)
![Immunohistochemistry-Paraffin: Rictor Antibody [NB100-612] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-Rictor used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunohistochemistry-Paraffin-NB100-612-img0019.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Rictor Antibody [NB100-612] - Detection of RICTOR on SKOV3 whole cell lysate, treated with PEITC. Image courtesy of product review by Parul Gupta (Texas Tech University Health Sciences Center).](http://images.novusbio.com/fullsize/Rictor-Antibody-Western-Blot-NB100-612-img0008.jpg)
![Western Blot: Rictor Antibody [NB100-612] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Rictor-Antibody-Western-Blot-NB100-612-img0021.jpg)
![Western Blot: Rictor Antibody [NB100-612] - mTOR complex protein expressions and rictor phosphorylation at Ser1235.Cardiomyocytes were pretreated for 30 min with 20 nM rapamycin and then stimulated with 10 nM IGF-1 in presence or absence of 10 nM E2 for 24 h. A, shown are representative western blots for mTOR, rictor and raptor; quantitative analysis was performed with mean +/- SEM of fold stimulation by IGF-1 of at least 3 independently performed experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123385), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rictor-Antibody-Western-Blot-NB100-612-img0023.jpg)
![Western Blot: Rictor Antibody [NB100-612] - Truncated Sin1 displaces endogenous Sin1 from mTORC2 in DLD1 colon cancer cellsA. Schematic indicating the domain structure of Sin1 & the constructs used to displace endogenous Sin1 from mTORC2. B. Expression of myc tagged Sin1 constructs can be detected only after induction with Doxycycline (Dox). Cells were treated with 100nM of doxycycline (+) for 72 hours & expressed proteins were detected by immunoblot of whole cell lysates with anti-myc (9E10) antibodies. C. & D. Sin1 constructs incorporate into mTORC2 & displace endogenous Sin1. Constructs were induced for 72 hours prior to immune precipitation. (C) mTORC2 subunits, mTOR & Rictor, only appear in myc immunoprecipitates after induction with doxycycline (Left panels); myc-∆Sin1 cannot be directly detected in precipitates due to secondary antibody cross reaction with precipitating IgG. Right panels indicate unchanging expression levels of Rictor & mTOR in immune precipitation input lysates, which is further quantified from 3 independent experiments E. Endogenous Sin1 & Rictor immunoprecipitates demonstrate displacement of endogenous Sin1 from mTORC2. Following induction, band shifted myc-tagged FL Sin1 can be detected in Sin1 & Rictor precipitates (Left panels). Truncated ∆Sin1 can be detected in Rictor, but not Sin1, immunoprecipitates as the Sin1 antibody epitope is deleted from ∆Sin1. F. Quantification of Sin1 levels detected in Rictor immunoprecipitates indicates the level of endogenous mTORC2 disruption following Sin1 construct induction (data are mean +/- S.D; n = 3). Myc-∆Sin1 displaces >80% of endogenous Sin1 while levels of myc-FL Sin1 associated with Rictor are comparable with endogenous Sin1 levels. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.20086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-612_rabbit-polyclonal-rictor-antibody-31020241554195.jpg)
![Western Blot: Rictor Antibody [NB100-612] - Truncated Sin1 displaces endogenous Sin1 from mTORC2 in DLD1 colon cancer cellsA. Schematic indicating the domain structure of Sin1 & the constructs used to displace endogenous Sin1 from mTORC2. B. Expression of myc tagged Sin1 constructs can be detected only after induction with Doxycycline (Dox). Cells were treated with 100nM of doxycycline (+) for 72 hours & expressed proteins were detected by immunoblot of whole cell lysates with anti-myc (9E10) antibodies. C. & D. Sin1 constructs incorporate into mTORC2 & displace endogenous Sin1. Constructs were induced for 72 hours prior to immune precipitation. (C) mTORC2 subunits, mTOR & Rictor, only appear in myc immunoprecipitates after induction with doxycycline (Left panels); myc-∆Sin1 cannot be directly detected in precipitates due to secondary antibody cross reaction with precipitating IgG. Right panels indicate unchanging expression levels of Rictor & mTOR in immune precipitation input lysates, which is further quantified from 3 independent experiments E. Endogenous Sin1 & Rictor immunoprecipitates demonstrate displacement of endogenous Sin1 from mTORC2. Following induction, band shifted myc-tagged FL Sin1 can be detected in Sin1 & Rictor precipitates (Left panels). Truncated ∆Sin1 can be detected in Rictor, but not Sin1, immunoprecipitates as the Sin1 antibody epitope is deleted from ∆Sin1. F. Quantification of Sin1 levels detected in Rictor immunoprecipitates indicates the level of endogenous mTORC2 disruption following Sin1 construct induction (data are mean +/- S.D; n = 3). Myc-∆Sin1 displaces >80% of endogenous Sin1 while levels of myc-FL Sin1 associated with Rictor are comparable with endogenous Sin1 levels. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.20086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-612_rabbit-polyclonal-rictor-antibody-31020241553710.jpg)
![Western Blot: Rictor Antibody [NB100-611] - Detection of Human Rictor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody NB100-611 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Rictor-Antibody-Western-Blot-NB100-611-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Rictor Antibody [NB100-611] - Detection of Human Rictor by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody NB100-611 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: Rictor Antibody [NB100-611] - Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rictor antibody NB100-611 (lot 5) used for IP at 6 ug per reaction. Rictor was also immunoprecipitated by a previous lot of this antibody (lot 4) and rabbit anti-Rictor antibody NB100-612. For blotting immunoprecipitated Rictor, NB100-611 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunoprecipitation-NB100-611-img0004.jpg)
![Western Blot: Rictor Antibody (1F3) [H00253260-M01] - RICTOR monoclonal antibody (M01), clone 1F3 Analysis of RICTOR expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Western-Blot-H00253260-M01-img0011.jpg "Western Blot: Rictor Antibody (1F3) [H00253260-M01] - RICTOR monoclonal antibody (M01), clone 1F3 Analysis of RICTOR expression in Hela S3 NE.")
![Immunohistochemistry-Paraffin: Rictor Antibody (1F3) [H00253260-M01] - Analysis of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Immunohistochemistry-Paraffin-H00253260-M01-img0008.jpg)
![Western Blot: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.Lane 1: RICTOR transfected lysate(29.8 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Western-Blot-H00253260-M01-img0012.jpg)
![Immunoprecipitation: Rictor Antibody (1F3) [H00253260-M01] - Analysis of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Immunoprecipitation-H00253260-M01-img0009.jpg)
![Sandwich ELISA: Rictor Antibody (1F3) [H00253260-M01] - Detection limit for recombinant GST tagged RICTOR is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Rictor-Antibody-1F3-Sandwich-ELISA-H00253260-M01-img0010.jpg)
![Western Blot: Rictor Antibody [NB100-56427] - Analysis of Rictor in Jurkat cell lysate using this antibody.](http://images.novusbio.com/fullsize/RICTOR-Antibody-Western-Blot-NB100-56427-img0002.jpg "Western Blot: Rictor Antibody [NB100-56427] - Analysis of Rictor in Jurkat cell lysate using this antibody.")
![Immunohistochemistry-Paraffin: Rictor Antibody [NB100-1534] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunohistochemistry-Paraffin-NB100-1534-img0002.jpg "Immunohistochemistry-Paraffin: Rictor Antibody [NB100-1534] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry: Rictor Antibody [NB100-1534] - The combined silencing of Rictor and EGFR in vivo results in a complete inhibition of tumor growth. U251Ng2x, U251Rictor, U251EGFR and U251EGFR/Rictor cells were implanted into the right caudate nucleus-putamen of Rag2M mice. Induction of shRNA expression in mice was initiated on day 21 by dissolving 2 mg/mL doxycyline and 5% sucrose in drinking water. On day 49, animals were imaged by Maestro (TM) fluorescence imaging unit expression of tRFP co-expressed with the shRNA sequences. Mice brains were harvested, sectioned and stained for nuclei, Rictor, EGFR and p(473)-AKT and imaged for all markers in addition to tRFP by robotic fluorescence microscopy. No tumor was detected in the U251EGFR/Rictor group. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0059597), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunohistochemistry-NB100-1534-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Rictor Antibody [NB100-1534] - Fluorescence micrographs showing EGFR (Alexa 488; green), Rictor (Alexa 488; yellow) & cell nuclei (Hoechst 33342; blue) in GBM4 GBM-derived cancer stem-like cell line, & Gli36, U251MG, U118MG & LN229 GBM cell lines. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23555046), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1534_goat-polyclonal-rictor-antibody-310202415392527.jpg)
![Immunocytochemistry/ Immunofluorescence: Rictor Antibody [NB100-1534] - The combined silencing of Rictor & EGFR in vivo results in a complete inhibition of tumor growth.U251Ng2x, U251Rictor, U251EGFR & U251EGFR/Rictor cells were implanted into the right caudate nucleus-putamen of Rag2M mice (n = 6−8). Induction of shRNA expression in mice was initiated on day 21 by dissolving 2 mg/mL doxycyline & 5% sucrose in drinking water. a) On day 49, animals were imaged by Maestro™ fluorescence imaging unit for the expression of tRFP co-expressed with the shRNA sequences upon doxycycline-induced expression. Mice were then terminated & brains were harvested, sectioned & stained for nuclei, Rictor, EGFR & p(473)-AKT & imaged for all markers in addition to tRFP by robotic fluorescence microscopy. No tumor was detected in the U251EGFR/Rictor group. b) A representative brain section from U251Ng2x, U251Rictor, U251EGFR & U251EGFR/Rictor tumor groups is shown: tRFP (red) & Hoechst (blue). c) A representative tumor section from U251Ng2x, U251Rictor & U251EGFR tumor groups is shown: nuclei (blue), rRFP (red), Rictor (yellow), EGFR (green) & p(473)-AKT (orange). d) The expression of EGFR (left axis), Rictor (right axis) & p(473)-AKT (right axis) in U251Ng2x, U251Rictor, U251EGFR tumor sections were quantified (positive staining normalized to Hoechst nuclei staining). e) Tumor sizes were estimated by quantification of tumor areas in brain sections from all groups (left axis). The expression of the proliferation marker Ki67 in the tumor (proliferating fraction) was also quantified (right axis). *p-value ≤0.05; **p-value ≤0.01; ***p-value ≤0.001 compared to control untreated cells. ‡: No tumor was detected in the U251EGFR/Rictor group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23555046), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1534_goat-polyclonal-rictor-antibody-310202415525248.jpg)
![Western Blot: Rictor Antibody (1A5) [NBP3-32903] - Western blot analysis of Rictor on HepG2 cell lysates with Rabbit anti-Rictor antibody (NBP3-32903) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 192 kDa Observed band size: 192 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32903) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32903_rabbit-rictor-mab-1a5-288202412442976.jpg "Western Blot: Rictor Antibody (1A5) [NBP3-32903] - Western blot analysis of Rictor on HepG2 cell lysates with Rabbit anti-Rictor antibody (NBP3-32903) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 192 kDa Observed band size: 192 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32903) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Rictor Antibody (1A5) [NBP3-32903] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Rictor antibody (NBP3-32903) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32903) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32903_rabbit-rictor-mab-1a5-298202414304175.jpg)
![Immunocytochemistry/ Immunofluorescence: Rictor Antibody (1A5) [NBP3-32903] - Immunocytochemistry analysis of A431 cells labeling Rictor with Rabbit anti-Rictor antibody (NBP3-32903) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Rictor antibody (NBP3-32903) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32903_rabbit-rictor-mab-1a5-29202410234282.jpg)
![Flow Cytometry: Rictor Antibody (1A5) [NBP3-32903] - Flow cytometric analysis of Hela cells labeling Rictor. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32903, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32903_rabbit-rictor-mab-1a5-29202416372198.jpg)
![Immunohistochemistry-Paraffin: Rictor Antibody [NBP2-38365] - Staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Rictor-Antibody-Immunohistochemistry-NBP2-38365-img0001.jpg "Immunohistochemistry-Paraffin: Rictor Antibody [NBP2-38365] - Staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.")