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![Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Western-Blot-NBP2-12913-img0003.jpg "Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK2 (pan) Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Immunocytochemistry-Immunofluorescence-NBP2-12913-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK2 (pan) Antibody. (D) Composite.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Immunocytochemistry-Immunofluorescence-NBP2-12913-img0005.jpg)
![Western Blot: SHANK2 Antibody [NBP3-13215] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK2 antibody (NBP3-13215) diluted at 1:1000.](http://images.novusbio.com/fullsize/SHANK2-Antibody-Western-Blot-NBP3-13215-img0001.jpg "Western Blot: SHANK2 Antibody [NBP3-13215] - Various tissue extracts (10 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK2 antibody (NBP3-13215) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody [NBP3-13215] - SHANK2 antibody detects SHANK2 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK2 protein stained by SHANK2 antibody (NBP3-13215) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SHANK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13215-img0003.jpg)
![Immunohistochemistry-Paraffin: SHANK2 Antibody [NBP3-13215] - SHANK2 antibody detects SHANK2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. SHANK2 antibody (NBP3-13215) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/SHANK2-Antibody-Immunohistochemistry-Paraffin-NBP3-13215-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody [NBP3-17310] - Staining of human cell line RT4 shows localization to nuclear speckles & vesicles.](http://images.novusbio.com/fullsize/SHANK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17310-img0001.jpg "Immunocytochemistry/Immunofluorescence: SHANK2 Antibody [NBP3-17310] - Staining of human cell line RT4 shows localization to nuclear speckles & vesicles.")