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![Western Blot: CEBP Beta Antibody (47A1) [NBP1-04266] - NIH 3T3 cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CEBP Beta; antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-47A1-Western-Blot-NBP1-04266-img0002.jpg "Western Blot: CEBP Beta Antibody (47A1) [NBP1-04266] - NIH 3T3 cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CEBP Beta; antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Western Blot: CEBP Beta Antibody (47A1) [NBP1-04266] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1 hour at room temperature. The membrane was probed with anti-CEBP beta 1:1000 in non-fat milk. This image was submitted via customer review.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-47A1-Western-Blot-NBP1-04266-img0003.jpg)
![Western Blot: CEBP Beta Antibody [NBP1-46179] - Detection of Human C/EBP beta by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug), 293T (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody NBP1-46179 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-Western-Blot-NBP1-46179-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: CEBP Beta Antibody [NBP1-46179] - Detection of Human C/EBP beta by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug), 293T (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody NBP1-46179 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CEBP Beta Antibody [NBP1-46179] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-C/EBP beta used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-Immunohistochemistry-Paraffin-NBP1-46179-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: CEBP Beta Antibody [NBP1-46179] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. C/EBP beta was also immunoprecipitated by rabbit anti-C/EBP beta antibody NBP1-46180, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-Western-Blot-NBP1-46179-img0001.jpg)
![Western Blot: CEBP Beta Antibody [NBP3-35083] - Western blot analysis of various lysates, using CEBP Beta Rabbit pAb at 1:700 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35083_rabbit-cebp-beta-pab-912202415133422.jpg "Western Blot: CEBP Beta Antibody [NBP3-35083] - Western blot analysis of various lysates, using CEBP Beta Rabbit pAb at 1:700 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunohistochemistry: CEBP Beta Antibody [NBP3-35083] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using CEBP Beta Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35083_rabbit-cebp-beta-pab-91220241519589.jpg)
![Immunohistochemistry: CEBP Beta Antibody [NBP3-35083] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using CEBP Beta Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35083_rabbit-cebp-beta-pab-912202415171192.jpg)
![Western Blot: CEBP Beta Antibody [NBP2-57075] - Analysis in human cell line RT-4, human cell line U-251 MG and human plasma.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-Western-Blot-NBP2-57075-img0002.jpg "Western Blot: CEBP Beta Antibody [NBP2-57075] - Analysis in human cell line RT-4, human cell line U-251 MG and human plasma.")
![Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody [NBP2-57075] - Staining of human cell line PC-3 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57075-img0001.jpg)
![Western Blot: CEBP Beta Antibody [NBP2-26206] - Staining of Human Skin (A) and Pig Lung (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-Western-Blot-NBP2-26206-img0002.jpg "Western Blot: CEBP Beta Antibody [NBP2-26206] - Staining of Human Skin (A) and Pig Lung (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: CEBP Beta Antibody [NBP2-26206] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-Western-Blot-NBP2-26206-img0001.jpg)
![SDS-Page: Recombinant Human CEBP Beta Protein [H00001051-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-CEBP-Beta-Protein-SDS-Page-H00001051-P01-img0002.jpg "SDS-Page: Recombinant Human CEBP Beta Protein [H00001051-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry: CEBP Beta Antibody (21A1) [NBP1-41216] - IHC of CEBP](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-21A1-Immunohistochemistry-NBP1-41216-img0001.jpg "Immunohistochemistry: CEBP Beta Antibody (21A1) [NBP1-41216] - IHC of CEBP")
![Western Blot: CEBP Beta Antibody (3H9) [NBP2-37567] - Western blot analysis using CEBPB mAb against human CEBPB (AA: 161-338) recombinant protein. (Expected MW is 44.5 kDa)](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-3H9-Western-Blot-NBP2-37567-img0002.jpg "Western Blot: CEBP Beta Antibody (3H9) [NBP2-37567] - Western blot analysis using CEBPB mAb against human CEBPB (AA: 161-338) recombinant protein. (Expected MW is 44.5 kDa)")
![Flow Cytometry: CEBP Beta Antibody (3H9) [NBP2-37567] - Flow cytometric analysis of MCF-7 cells using CEBPB mouse mAb (blue) and negative control (red).](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-3H9-Flow-Cytometry-NBP2-37567-img0003.jpg)
![ELISA: CEBP Beta Antibody (3H9) [NBP2-37567] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-3H9-ELISA-NBP2-37567-img0001.jpg)
![Western Blot: CEBP Beta Antibody (9W4A10) [NBP3-15761] - Western blot analysis of extracts of various cell lines, using CEBP Beta antibody (NBP3-15761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-9W4A10-Western-Blot-NBP3-15761-img0001.jpg "Western Blot: CEBP Beta Antibody (9W4A10) [NBP3-15761] - Western blot analysis of extracts of various cell lines, using CEBP Beta antibody (NBP3-15761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: CEBP Beta [p Thr235] Antibody [NBP3-16923] - Western blot analysis of extracts of NIH/3T3, using CEBP Beta antibody (NBP3-16923) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.NIH/3T3 cells were treated by Serum-starvation overnight at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/CEBP-Beta-[p-Thr235]-Antibody-Western-Blot-NBP3-16923-img0001.jpg "Western Blot: CEBP Beta [p Thr235] Antibody [NBP3-16923] - Western blot analysis of extracts of NIH/3T3, using CEBP Beta antibody (NBP3-16923) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour.NIH/3T3 cells were treated by Serum-starvation overnight at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody [NBP2-56476] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56476-img0001.jpg "Immunocytochemistry/Immunofluorescence: CEBP Beta Antibody [NBP2-56476] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![ELISA: Human CEBP Beta ELISA Kit (Colorimetric) [NBP2-70025] - Samples were spiked with high concentrations of Human CEBP Beta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-70025_human-cebp-beta-elisa-kit-colorimetric-132202416175688.jpg "ELISA: Human CEBP Beta ELISA Kit (Colorimetric) [NBP2-70025] - Samples were spiked with high concentrations of Human CEBP Beta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human CEBP Beta ELISA Kit (Colorimetric) [NBP2-70025] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-70025_human-cebp-beta-elisa-kit-colorimetric-43202485678.jpg)