![Western Blot: CEBP Beta Antibody [NBP1-46179] - Detection of Human C/EBP beta by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug), 293T (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody NBP1-46179 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-Western-Blot-NBP1-46179-img0006.jpg "Western Blot: CEBP Beta Antibody [NBP1-46179] - Detection of Human C/EBP beta by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug), 293T (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody NBP1-46179 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen for this product maps to a region between residue 50 and 100 of human CCAAT/Enhancer Binding Protein (C/EBP), beta using the numbering given in entry NP_005185.2 (GeneID 1051). |
| Predicted Species | Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CEBPB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Immunocytochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunocytochemistry__Immunofluorescence_21634.jpg)
![Immunohistochemistry FosB/G0S3 Antibody [Unconjugated]](https://images.novusbio.com/images2/FosB_AF2214_Immunohistochemistry_9938.jpg)
| Publications using NBP1-46179 | Applications | Species |
|---|---|---|
| Dickson MJ, Sheldon IM, Bromfield JJ. Lipopolysaccharide alters CEBP? signaling and reduces estradiol production in bovine granulosa cells CABI Agriculture and Bioscience 2022-10-22 [PMID: 37576606] | ||
| Ke Y, Li BZ, Nguyen K et al. IL22RA2 is a Smad7 target mediating alleviation of dermatitis and psoriatic phenotypes in mice The Journal of investigative dermatology 2023-05-19 [PMID: 37211203] (Western Blot, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Mouse) | Western Blot, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for CEBP Beta Antibody (NBP1-46179)Find related products by research area.
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Transcription Factor Antibodies Used In Landmark Evolutionary Study We at Novus Biologicals offer a full antibody database targeted to transcription factor research. Recently, CEBP antibodieswere used in a research study exploring the evolution of gene regulation in various vertebrates. The results revealed surprising... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CEBPB |
![Immunohistochemistry-Paraffin: CEBP Beta Antibody [NBP1-46179] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-C/EBP beta used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-Immunohistochemistry-Paraffin-NBP1-46179-img0004.jpg "Immunohistochemistry-Paraffin: CEBP Beta Antibody [NBP1-46179] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-C/EBP beta used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .")
![Western Blot: CEBP Beta Antibody [NBP1-46179] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. C/EBP beta was also immunoprecipitated by rabbit anti-C/EBP beta antibody NBP1-46180, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/CEBP-Beta-Antibody-Western-Blot-NBP1-46179-img0001.jpg "Western Blot: CEBP Beta Antibody [NBP1-46179] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. C/EBP beta was also immunoprecipitated by rabbit anti-C/EBP beta antibody NBP1-46180, which recognizes a downstream epitope.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")