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![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining in human tonsil and cerebral cortex tissues using anti-IKZF1 antibody. Corresponding IKZF1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38242-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining in human tonsil and cerebral cortex tissues using anti-IKZF1 antibody. Corresponding IKZF1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining of human cerebral cortex, kidney, testis and tonsil using Anti-IKZF1 antibody NBP2-38242 (A) shows similar protein distribution across tissues to independent antibody NBP2-38241 (B).](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38242-img0011.jpg)
![Western Blot: Ikaros/IKZF1 Antibody [NBP2-38242] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Western-Blot-NBP2-38242-img0002.jpg)
![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38242-img0008.jpg)
![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38242-img0009.jpg)
![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining of human kidney using Anti-IKZF1 antibody.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38242-img0012.jpg)
![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38242] - Staining of human testis using Anti-IKZF1 antibody.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38242-img0013.jpg)
![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38241] - Staining in human tonsil and cerebral cortex tissues using anti-IKZF1 antibody. Corresponding IKZF1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38241-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38241] - Staining in human tonsil and cerebral cortex tissues using anti-IKZF1 antibody. Corresponding IKZF1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38241] - Staining of human cerebral cortex, kidney, testis and tonsil using Anti-IKZF1 antibody NBP2-38241 (A) shows similar protein distribution across tissues to independent antibody NBP2-38242 (B).](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38241-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Ikaros/IKZF1 Antibody [NBP2-38241] - Staining of human cell line REH shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38241-img0006.jpg)
![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38241] - Staining of human testis.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38241-img0008.jpg)
![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38241] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38241-img0002.jpg)
![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38241] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38241-img0003.jpg)
![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP2-38241] - Staining of human kidney.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38241-img0009.jpg)
![Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Analysis of extracts of various cell lines, using Ikaros/IKZF1 Rabbit mAb (NBP3-16223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-4S10I3-Western-Blot-NBP3-16223-img0002.jpg "Western Blot: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Analysis of extracts of various cell lines, using Ikaros/IKZF1 Rabbit mAb (NBP3-16223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Immunofluorescence analysis of Jurkat cells using Ikaros/IKZF1 Rabbit mAb (NBP3-16223) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-4S10I3-Immunocytochemistry-Immunofluorescence-NBP3-16223-img0001.jpg)
![Chromatin Immunoprecipitation: Ikaros/IKZF1 Antibody (4S10I3) [NBP3-16223] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Ikaros/IKZF1 antibody (NBP3-16223) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-4S10I3-Chromatin-Immunoprecipitation-NBP3-16223-img0003.jpg)
![Western Blot: Ikaros/IKZF1 Antibody [NBP1-98314] - Mouse Small Intestine Lysate 1.0ug/ml, Gel Concentration: 12%](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Western-Blot-NBP1-98314-img0002.jpg "Western Blot: Ikaros/IKZF1 Antibody [NBP1-98314] - Mouse Small Intestine Lysate 1.0ug/ml, Gel Concentration: 12%")
![Western Blot: Ikaros/IKZF1 Antibody (S08-5E4) [NBP3-19660] - Western blot detection of Ikaros/IKZF1 in CHO-K1, Jurkat cell lysates using NBP3-19660 (1:500 diluted). Predicted band size: 58kDa. Observed band size: 50-70kDa.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-S08-5E4-Western-Blot-NBP3-19660-img0002.jpg "Western Blot: Ikaros/IKZF1 Antibody (S08-5E4) [NBP3-19660] - Western blot detection of Ikaros/IKZF1 in CHO-K1, Jurkat cell lysates using NBP3-19660 (1:500 diluted). Predicted band size: 58kDa. Observed band size: 50-70kDa.")
![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody (S08-5E4) [NBP3-19660] - Immunohistochemistry of Ikaros/IKZF1 in paraffin-embedded Human tonsil using NBP3-19660 at dilution 1/50](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-S08-5E4-Immunohistochemistry-Paraffin-NBP3-19660-img0001.jpg)
![Flow Cytometry: Mouse Monoclonal Ikaros/IKZF1 Antibody (4E9) [NBP3-09111] - Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Ikaros (4E9) purified antibody (concentration in sample 8 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/antibody/nbp3-09111_mouse-monoclonal-ikaros-ikzf1-antibody-4e9-flow-cytometry-1212202415710..jpg "Flow Cytometry: Mouse Monoclonal Ikaros/IKZF1 Antibody (4E9) [NBP3-09111] - Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-Ikaros (4E9) purified antibody (concentration in sample 8 ug/ml, GAM APC).")
![Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP1-51990] - (3.8ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/nbp1-51990_goat-polyclonal-ikaros-ikzf1-antibody-imgenex-img-31241-187202311342923.jpg "Immunohistochemistry-Paraffin: Ikaros/IKZF1 Antibody [NBP1-51990] - (3.8ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Western Blot: Ikaros/IKZF1 Antibody [NBP1-00125] - (0.03ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Western-Blot-NBP1-00125-img0003.jpg "Western Blot: Ikaros/IKZF1 Antibody [NBP1-00125] - (0.03ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Ikaros/IKZF1 Antibody [NBP1-98565] - Mouse Heart lysate, concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Ikaros-IKZF1-Antibody-Western-Blot-NBP1-98565-img0002.jpg "Western Blot: Ikaros/IKZF1 Antibody [NBP1-98565] - Mouse Heart lysate, concentration 1 ug/ml.")
![Flow Cytometry: Ikaros/IKZF1 Antibody (21E9) [NBP3-26204] - Overlay Peak curve showing Jurkat cells stained with Ikaros/IKZF1 Antibody (21E9) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26204_rabbit-ikaros-ikzf1-mab-21e9-282202414312790.jpg "Flow Cytometry: Ikaros/IKZF1 Antibody (21E9) [NBP3-26204] - Overlay Peak curve showing Jurkat cells stained with Ikaros/IKZF1 Antibody (21E9) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.")